H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions
Background: The histone variant H2A.Z has been implicated in nucleosome exchange, transcriptional activation and Polycomb repression. However, the relationships among these seemingly disparate functions remain obscure. Results: We mapped H2A.Z genome-wide in mammalian ES cells and neural progenitor...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Biomed Central Ltd.
2013
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| Online Access: | http://hdl.handle.net/1721.1/76688 |
| _version_ | 1826195051933335552 |
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| author | Ku, Manching Jaffe, Jacob D. Koche, Richard Patrick Rheinbay, Esther Endoh, Mitsuhiro Koseki, Haruhiko Carr, Steven A. Bernstein, Bradley E. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Ku, Manching Jaffe, Jacob D. Koche, Richard Patrick Rheinbay, Esther Endoh, Mitsuhiro Koseki, Haruhiko Carr, Steven A. Bernstein, Bradley E. |
| author_sort | Ku, Manching |
| collection | MIT |
| description | Background: The histone variant H2A.Z has been implicated in nucleosome exchange, transcriptional activation and Polycomb repression. However, the relationships among these seemingly disparate functions remain obscure.
Results: We mapped H2A.Z genome-wide in mammalian ES cells and neural progenitors. H2A.Z is deposited promiscuously at promoters and enhancers, and correlates strongly with H3K4 methylation. Accordingly, H2A.Z is present at poised promoters with bivalent chromatin and at active promoters with H3K4 methylation, but is absent from stably repressed promoters that are specifically enriched for H3K27 trimethylation. We also characterized post-translational modification states of H2A.Z, including a novel species dually-modified by ubiquitination and acetylation that is enriched at bivalent chromatin.
Conclusions: Our findings associate H2A.Z with functionally distinct genomic elements, and suggest that post-translational modifications may reconcile its contrasting locations and roles. |
| first_indexed | 2024-09-23T10:06:07Z |
| format | Article |
| id | mit-1721.1/76688 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:06:07Z |
| publishDate | 2013 |
| publisher | Biomed Central Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/766882022-09-26T15:41:58Z H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions Ku, Manching Jaffe, Jacob D. Koche, Richard Patrick Rheinbay, Esther Endoh, Mitsuhiro Koseki, Haruhiko Carr, Steven A. Bernstein, Bradley E. Harvard University--MIT Division of Health Sciences and Technology Koche, Richard Patrick Background: The histone variant H2A.Z has been implicated in nucleosome exchange, transcriptional activation and Polycomb repression. However, the relationships among these seemingly disparate functions remain obscure. Results: We mapped H2A.Z genome-wide in mammalian ES cells and neural progenitors. H2A.Z is deposited promiscuously at promoters and enhancers, and correlates strongly with H3K4 methylation. Accordingly, H2A.Z is present at poised promoters with bivalent chromatin and at active promoters with H3K4 methylation, but is absent from stably repressed promoters that are specifically enriched for H3K27 trimethylation. We also characterized post-translational modification states of H2A.Z, including a novel species dually-modified by ubiquitination and acetylation that is enriched at bivalent chromatin. Conclusions: Our findings associate H2A.Z with functionally distinct genomic elements, and suggest that post-translational modifications may reconcile its contrasting locations and roles. 2013-01-30T21:06:20Z 2013-01-30T21:06:20Z 2012-10 2012-07 Article http://purl.org/eprint/type/JournalArticle 1465-6906 1474-7596 http://hdl.handle.net/1721.1/76688 Ku, Manching et al. “H2A.Z Landscapes and Dual Modifications in Pluripotent and Multipotent Stem Cells Underlie Complex Genome Regulatory Functions.” Genome Biology 13.10 (2012): R85. en_US http://dx.doi.org/10.1186/gb-2012-13-10-r85 Genome Biology Creative Commons Attribution http://creativecommons.org/licenses/by/2.0 application/pdf Biomed Central Ltd. BioMed Central |
| spellingShingle | Ku, Manching Jaffe, Jacob D. Koche, Richard Patrick Rheinbay, Esther Endoh, Mitsuhiro Koseki, Haruhiko Carr, Steven A. Bernstein, Bradley E. H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions |
| title | H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions |
| title_full | H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions |
| title_fullStr | H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions |
| title_full_unstemmed | H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions |
| title_short | H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions |
| title_sort | h2a z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions |
| url | http://hdl.handle.net/1721.1/76688 |
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