Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems
The physiology of the Drosophila melanogaster cardiovascular system remains poorly characterized compared with its vertebrate counterparts. Basic measures of physiological performance remain unknown. It also is unclear whether subtle physiological defects observed in the human cardiovascular system...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en_US |
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Company of Biologists Ltd.
2013
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| Online Access: | http://hdl.handle.net/1721.1/76783 |
| _version_ | 1811075889144791040 |
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| author | Choma, Michael A. Suter, Melissa J. Vakoc, Benjamin Bouma, Brett E. Tearney, Guillermo J. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Choma, Michael A. Suter, Melissa J. Vakoc, Benjamin Bouma, Brett E. Tearney, Guillermo J. |
| author_sort | Choma, Michael A. |
| collection | MIT |
| description | The physiology of the Drosophila melanogaster cardiovascular system remains poorly characterized compared with its vertebrate counterparts. Basic measures of physiological performance remain unknown. It also is unclear whether subtle physiological defects observed in the human cardiovascular system can be reproduced in D. melanogaster. Here we characterize the cardiovascular physiology of D. melanogaster in its pre-pupal stage by using high-speed dye angiography and optical coherence tomography. The heart has vigorous pulsatile contractions that drive intracardiac, aortic and extracellular-extravascular hemolymph flow. Several physiological measures, including weight-adjusted cardiac output, body-length-adjusted aortic velocities and intracardiac shear forces, are similar to those in the closed vertebrate cardiovascular systems, including that of humans. Extracellular-extravascular flow in the pre-pupal D. melanogaster circulation drives convection-limited fluid transport. To demonstrate homology in heart dysfunction, we showed that, at the pre-pupal stage, a troponin I mutant, held-up2 (hdp2), has impaired systolic and diastolic heart wall velocities. Impaired heart wall velocities occur in the context of a non-dilated phenotype with a mildly depressed fractional shortening. We additionally derive receiver operating characteristic curves showing that heart wall velocity is a potentially powerful discriminator of systolic heart dysfunction. Our results demonstrate physiological homology and support the use of D. melanogaster as an animal model of complex cardiovascular disease. |
| first_indexed | 2024-09-23T10:13:25Z |
| format | Article |
| id | mit-1721.1/76783 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:13:25Z |
| publishDate | 2013 |
| publisher | Company of Biologists Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/767832022-09-30T19:42:31Z Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems Choma, Michael A. Suter, Melissa J. Vakoc, Benjamin Bouma, Brett E. Tearney, Guillermo J. Harvard University--MIT Division of Health Sciences and Technology Vakoc, Benjamin Bouma, Brett E. Tearney, Guillermo J. The physiology of the Drosophila melanogaster cardiovascular system remains poorly characterized compared with its vertebrate counterparts. Basic measures of physiological performance remain unknown. It also is unclear whether subtle physiological defects observed in the human cardiovascular system can be reproduced in D. melanogaster. Here we characterize the cardiovascular physiology of D. melanogaster in its pre-pupal stage by using high-speed dye angiography and optical coherence tomography. The heart has vigorous pulsatile contractions that drive intracardiac, aortic and extracellular-extravascular hemolymph flow. Several physiological measures, including weight-adjusted cardiac output, body-length-adjusted aortic velocities and intracardiac shear forces, are similar to those in the closed vertebrate cardiovascular systems, including that of humans. Extracellular-extravascular flow in the pre-pupal D. melanogaster circulation drives convection-limited fluid transport. To demonstrate homology in heart dysfunction, we showed that, at the pre-pupal stage, a troponin I mutant, held-up2 (hdp2), has impaired systolic and diastolic heart wall velocities. Impaired heart wall velocities occur in the context of a non-dilated phenotype with a mildly depressed fractional shortening. We additionally derive receiver operating characteristic curves showing that heart wall velocity is a potentially powerful discriminator of systolic heart dysfunction. Our results demonstrate physiological homology and support the use of D. melanogaster as an animal model of complex cardiovascular disease. Boston Children's Hospital (Frederick H. Lovejoy Fund) American Medical Association (Seed Grant) National Institutes of Health (U.S.) (NIH R01HL076398) National Institutes of Health (U.S.) (NIH R01CA103769) United States. Air Force Office of Scientific Research (Medical Free Electron Laser Program FA9550-04-1-0079) 2013-02-12T20:24:22Z 2013-02-12T20:24:22Z 2010-12 2010-01 Article http://purl.org/eprint/type/JournalArticle 1754-8403 1754-8411 http://hdl.handle.net/1721.1/76783 Choma, M. A. et al. “Physiological Homology Between Drosophila Melanogaster and Vertebrate Cardiovascular Systems.” Disease Models & Mechanisms 4.3 (2010): 411–420. Web. en_US http://dx.doi.org/10.1242/dmm.005231 Disease Models and Mechanisms Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Company of Biologists Ltd. Company of Biologists |
| spellingShingle | Choma, Michael A. Suter, Melissa J. Vakoc, Benjamin Bouma, Brett E. Tearney, Guillermo J. Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
| title | Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
| title_full | Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
| title_fullStr | Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
| title_full_unstemmed | Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
| title_short | Physiological homology between Drosophila melanogaster and vertebrate cardiovascular systems |
| title_sort | physiological homology between drosophila melanogaster and vertebrate cardiovascular systems |
| url | http://hdl.handle.net/1721.1/76783 |
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