Retroactivity, modularity, and insulation in synthetic biology circuits
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2011.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2013
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| Online Access: | http://hdl.handle.net/1721.1/76989 |
| _version_ | 1811085446058344448 |
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| author | Lin, Allen |
| author2 | Ron Weiss and Domitilla Del Vecchio. |
| author_facet | Ron Weiss and Domitilla Del Vecchio. Lin, Allen |
| author_sort | Lin, Allen |
| collection | MIT |
| description | Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2011. |
| first_indexed | 2024-09-23T13:09:43Z |
| format | Thesis |
| id | mit-1721.1/76989 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T13:09:43Z |
| publishDate | 2013 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/769892019-04-10T09:10:32Z Retroactivity, modularity, and insulation in synthetic biology circuits Modularity, Retroactivity, and insulation in synthetic biology circuits Lin, Allen Ron Weiss and Domitilla Del Vecchio. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2011. Cataloged from PDF version of thesis. Includes bibliographical references (p. 141-151). A central concept in synthetic biology is the reuse of well-characterized modules. Modularity simplifies circuit design by allowing for the decomposition of systems into separate modules for individual construction. Complex regulatory networks can be assembled from a library of devices. However, current devices in synthetic biology may not actually be modular and may instead change behavior upon interconnections, a phenomenon called retroactivity. Addition of a new component to a system can change individual device dynamics within the system, potentially making timeconsuming iterative redesign necessary. Another need for systems construction is the ability to rapidly assemble constructs from part libraries in a combinatorial, highthroughput fashion. In this thesis, a multi-site assembly method that permits the rapid reshuffling of promoters and genes for yeast expression is established. Synthetic circuits in yeast to measure retroactivity and to act as an insulator that attenuates such effect are designed and modeled. by Allen Lin. M.Eng. 2013-02-14T15:35:43Z 2013-02-14T15:35:43Z 2011 2011 Thesis http://hdl.handle.net/1721.1/76989 825553696 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 151 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science. Lin, Allen Retroactivity, modularity, and insulation in synthetic biology circuits |
| title | Retroactivity, modularity, and insulation in synthetic biology circuits |
| title_full | Retroactivity, modularity, and insulation in synthetic biology circuits |
| title_fullStr | Retroactivity, modularity, and insulation in synthetic biology circuits |
| title_full_unstemmed | Retroactivity, modularity, and insulation in synthetic biology circuits |
| title_short | Retroactivity, modularity, and insulation in synthetic biology circuits |
| title_sort | retroactivity modularity and insulation in synthetic biology circuits |
| topic | Electrical Engineering and Computer Science. |
| url | http://hdl.handle.net/1721.1/76989 |
| work_keys_str_mv | AT linallen retroactivitymodularityandinsulationinsyntheticbiologycircuits AT linallen modularityretroactivityandinsulationinsyntheticbiologycircuits |