Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo
Background: A major hurdle in the use of exogenous stems cells for therapeutic regeneration of injured myocardium remains the poor survival of implanted cells. To date, the delivery of stem cells into myocardium has largely focused on implantation of cell suspensions. Methodology and Principal Findi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2013
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| Online Access: | http://hdl.handle.net/1721.1/77204 |
| _version_ | 1811075992864686080 |
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| author | Bauer, Michael Kang, Lifeng Qiu, Yiling Peng, Michelle Chen, Howard H. Camci-Unal, Gulden Bayomy, Ahmad F. Sosnovik, David E. Khademhosseini, Ali Liao, Ronglih |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Bauer, Michael Kang, Lifeng Qiu, Yiling Peng, Michelle Chen, Howard H. Camci-Unal, Gulden Bayomy, Ahmad F. Sosnovik, David E. Khademhosseini, Ali Liao, Ronglih |
| author_sort | Bauer, Michael |
| collection | MIT |
| description | Background: A major hurdle in the use of exogenous stems cells for therapeutic regeneration of injured myocardium remains the poor survival of implanted cells. To date, the delivery of stem cells into myocardium has largely focused on implantation of cell suspensions. Methodology and Principal Findings: We hypothesize that delivering progenitor cells in an aggregate form would serve to mimic the endogenous state with proper cell-cell contact, and may aid the survival of implanted cells. Microwell methodologies allow for the culture of homogenous 3D cell aggregates, thereby allowing cell-cell contact. In this study, we find that the culture of cardiac progenitor cells in a 3D cell aggregate augments cell survival and protects against cellular toxins and stressors, including hydrogen peroxide and anoxia/reoxygenation induced cell death. Moreover, using a murine model of cardiac ischemia-reperfusion injury, we find that delivery of cardiac progenitor cells in the form of 3D aggregates improved in vivo survival of implanted cells. Conclusion: Collectively, our data support the notion that growth in 3D cellular systems and maintenance of cell-cell contact improves exogenous cell survival following delivery into myocardium. These approaches may serve as a strategy to improve cardiovascular cell-based therapies. |
| first_indexed | 2024-09-23T10:14:28Z |
| format | Article |
| id | mit-1721.1/77204 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:14:28Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/772042022-09-26T16:39:57Z Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo Bauer, Michael Kang, Lifeng Qiu, Yiling Peng, Michelle Chen, Howard H. Camci-Unal, Gulden Bayomy, Ahmad F. Sosnovik, David E. Khademhosseini, Ali Liao, Ronglih Harvard University--MIT Division of Health Sciences and Technology Wu, Jinhui Kang, Lifeng Wu, Jinhui Peng, Michelle Camci-Unal, Gulden Sosnovik, David E. Khademhosseini, Ali Background: A major hurdle in the use of exogenous stems cells for therapeutic regeneration of injured myocardium remains the poor survival of implanted cells. To date, the delivery of stem cells into myocardium has largely focused on implantation of cell suspensions. Methodology and Principal Findings: We hypothesize that delivering progenitor cells in an aggregate form would serve to mimic the endogenous state with proper cell-cell contact, and may aid the survival of implanted cells. Microwell methodologies allow for the culture of homogenous 3D cell aggregates, thereby allowing cell-cell contact. In this study, we find that the culture of cardiac progenitor cells in a 3D cell aggregate augments cell survival and protects against cellular toxins and stressors, including hydrogen peroxide and anoxia/reoxygenation induced cell death. Moreover, using a murine model of cardiac ischemia-reperfusion injury, we find that delivery of cardiac progenitor cells in the form of 3D aggregates improved in vivo survival of implanted cells. Conclusion: Collectively, our data support the notion that growth in 3D cellular systems and maintenance of cell-cell contact improves exogenous cell survival following delivery into myocardium. These approaches may serve as a strategy to improve cardiovascular cell-based therapies. 2013-02-27T15:13:23Z 2013-02-27T15:13:23Z 2012-11 2012-09 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/77204 Bauer, Michael et al. “Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo.” Ed. Loren E. Wold. PLoS ONE 7.11 (2012). en_US http://dx.doi.org/10.1371/journal.pone.0050491 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Bauer, Michael Kang, Lifeng Qiu, Yiling Peng, Michelle Chen, Howard H. Camci-Unal, Gulden Bayomy, Ahmad F. Sosnovik, David E. Khademhosseini, Ali Liao, Ronglih Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo |
| title | Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo |
| title_full | Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo |
| title_fullStr | Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo |
| title_full_unstemmed | Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo |
| title_short | Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo |
| title_sort | adult cardiac progenitor cell aggregates exhibit survival benefit both in vitro and in vivo |
| url | http://hdl.handle.net/1721.1/77204 |
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