Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria
Our understanding of secondary metabolite production in bacteria has been shaped primarily by studies of attached varieties such as symbionts, pathogens, and soil bacteria. Here we show that a strain of the single-celled, planktonic marine cyanobacterium Prochlorococcus—which conducts a sizable frac...
| Prif Awduron: | , , , , , , , , , |
|---|---|
| Awduron Eraill: | |
| Fformat: | Erthygl |
| Iaith: | en_US |
| Cyhoeddwyd: |
National Academy of Sciences (U.S.)
2013
|
| Mynediad Ar-lein: | http://hdl.handle.net/1721.1/77585 https://orcid.org/0000-0001-6164-5126 |
| _version_ | 1826217780036239360 |
|---|---|
| author | Li, Bo Sher, Daniel Kelly, Libusha Shi, Yanxiang Huang, Katherine H. Knerr, Patrick J. Joewono, Ike Rusch, Doug Chisholm, Sallie (Penny) van der Donk, Wilfred A. |
| author2 | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Li, Bo Sher, Daniel Kelly, Libusha Shi, Yanxiang Huang, Katherine H. Knerr, Patrick J. Joewono, Ike Rusch, Doug Chisholm, Sallie (Penny) van der Donk, Wilfred A. |
| author_sort | Li, Bo |
| collection | MIT |
| description | Our understanding of secondary metabolite production in bacteria has been shaped primarily by studies of attached varieties such as symbionts, pathogens, and soil bacteria. Here we show that a strain of the single-celled, planktonic marine cyanobacterium Prochlorococcus—which conducts a sizable fraction of photosynthesis in the oceans—produces many cyclic, lanthionine-containing peptides (lantipeptides). Remarkably, in Prochlorococcus MIT9313 a single promiscuous enzyme transforms up to 29 different linear ribosomally synthesized peptides into a library of polycyclic, conformationally constrained products with highly diverse ring topologies. Genes encoding this system are found in variable abundances across the oceans—with a hot spot in a Galapagos hypersaline lagoon—suggesting they play a habitat- and/or community-specific role. The extraordinarily efficient pathway for generating structural diversity enables these cyanobacteria to produce as many secondary metabolites as model antibiotic-producing bacteria, but with much smaller genomes. |
| first_indexed | 2024-09-23T17:09:02Z |
| format | Article |
| id | mit-1721.1/77585 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T17:09:02Z |
| publishDate | 2013 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/775852022-09-30T00:00:17Z Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria Li, Bo Sher, Daniel Kelly, Libusha Shi, Yanxiang Huang, Katherine H. Knerr, Patrick J. Joewono, Ike Rusch, Doug Chisholm, Sallie (Penny) van der Donk, Wilfred A. Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Kelly, Libusha Sher, Daniel Huang, Katherine H. Chisholm, Sallie (Penny) Our understanding of secondary metabolite production in bacteria has been shaped primarily by studies of attached varieties such as symbionts, pathogens, and soil bacteria. Here we show that a strain of the single-celled, planktonic marine cyanobacterium Prochlorococcus—which conducts a sizable fraction of photosynthesis in the oceans—produces many cyclic, lanthionine-containing peptides (lantipeptides). Remarkably, in Prochlorococcus MIT9313 a single promiscuous enzyme transforms up to 29 different linear ribosomally synthesized peptides into a library of polycyclic, conformationally constrained products with highly diverse ring topologies. Genes encoding this system are found in variable abundances across the oceans—with a hot spot in a Galapagos hypersaline lagoon—suggesting they play a habitat- and/or community-specific role. The extraordinarily efficient pathway for generating structural diversity enables these cyanobacteria to produce as many secondary metabolites as model antibiotic-producing bacteria, but with much smaller genomes. Howard Hughes Medical Institute United States-Israel Binational Science Foundation (Agricultural Research and Development Fund (Vaadia-BARD Postdoctoral Fellowship Award FI-399-2007)) Fulbright Program National Institutes of Health (U.S.) (grant GM58822) United States. Dept. of Energy (Genomics:GTL Program) National Science Foundation (U.S.) Gordon and Betty Moore Foundation 2013-03-06T19:46:21Z 2013-03-06T19:46:21Z 2010-05 2009-12 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/77585 Li, B. et al. “Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria.” Proceedings of the National Academy of Sciences 107.23 (2010): 10430–10435. CrossRef. Web. https://orcid.org/0000-0001-6164-5126 en_US http://dx.doi.org/10.1073/pnas.0913677107 Proceedings of the National Academy of Sciences of the United States of America Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Li, Bo Sher, Daniel Kelly, Libusha Shi, Yanxiang Huang, Katherine H. Knerr, Patrick J. Joewono, Ike Rusch, Doug Chisholm, Sallie (Penny) van der Donk, Wilfred A. Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria |
| title | Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria |
| title_full | Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria |
| title_fullStr | Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria |
| title_full_unstemmed | Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria |
| title_short | Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria |
| title_sort | catalytic promiscuity in the biosynthesis of cyclic peptide secondary metabolites in planktonic marine cyanobacteria |
| url | http://hdl.handle.net/1721.1/77585 https://orcid.org/0000-0001-6164-5126 |
| work_keys_str_mv | AT libo catalyticpromiscuityinthebiosynthesisofcyclicpeptidesecondarymetabolitesinplanktonicmarinecyanobacteria AT sherdaniel catalyticpromiscuityinthebiosynthesisofcyclicpeptidesecondarymetabolitesinplanktonicmarinecyanobacteria AT kellylibusha catalyticpromiscuityinthebiosynthesisofcyclicpeptidesecondarymetabolitesinplanktonicmarinecyanobacteria AT shiyanxiang catalyticpromiscuityinthebiosynthesisofcyclicpeptidesecondarymetabolitesinplanktonicmarinecyanobacteria AT huangkatherineh catalyticpromiscuityinthebiosynthesisofcyclicpeptidesecondarymetabolitesinplanktonicmarinecyanobacteria AT knerrpatrickj catalyticpromiscuityinthebiosynthesisofcyclicpeptidesecondarymetabolitesinplanktonicmarinecyanobacteria AT joewonoike catalyticpromiscuityinthebiosynthesisofcyclicpeptidesecondarymetabolitesinplanktonicmarinecyanobacteria AT ruschdoug catalyticpromiscuityinthebiosynthesisofcyclicpeptidesecondarymetabolitesinplanktonicmarinecyanobacteria AT chisholmsalliepenny catalyticpromiscuityinthebiosynthesisofcyclicpeptidesecondarymetabolitesinplanktonicmarinecyanobacteria AT vanderdonkwilfreda catalyticpromiscuityinthebiosynthesisofcyclicpeptidesecondarymetabolitesinplanktonicmarinecyanobacteria |