Engineered Contractile Skeletal Muscle Tissue on a Microgrooved Methacrylated Gelatin Substrate
To engineer tissue-like structures, cells must organize themselves into three-dimensional (3D) networks that mimic the native tissue microarchitecture. Microfabricated hydrogel substrates provide a potentially useful platform for directing cells into biomimetic tissue architecture in vitro. Here, we...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert, Inc.
2013
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| Online Access: | http://hdl.handle.net/1721.1/77632 |
| _version_ | 1811071032290705408 |
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| author | Hosseini, Vahid Ahadian, Samad Ostrovidov, Serge Camci-Unal, Gulden Chen, Song Kaji, Hirokazu Ramalingam, Murugan Khademhosseini, Ali |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Hosseini, Vahid Ahadian, Samad Ostrovidov, Serge Camci-Unal, Gulden Chen, Song Kaji, Hirokazu Ramalingam, Murugan Khademhosseini, Ali |
| author_sort | Hosseini, Vahid |
| collection | MIT |
| description | To engineer tissue-like structures, cells must organize themselves into three-dimensional (3D) networks that mimic the native tissue microarchitecture. Microfabricated hydrogel substrates provide a potentially useful platform for directing cells into biomimetic tissue architecture in vitro. Here, we present microgrooved methacrylated gelatin hydrogels as a suitable platform to build muscle-like fibrous structures in a facile and highly reproducible fashion. Microgrooved hydrogel substrates with two different ridge sizes (50 and 100 μm) were fabricated to assess the effect of the distance between engineered myofibers on the orientation of the bridging C2C12 myoblasts and the formation of the resulting multinucleated myotubes. It was shown that although the ridge size did not significantly affect the C2C12 myoblast alignment, the wider-ridged micropatterned hydrogels generated more myotubes that were not aligned to the groove direction as compared to those on the smaller-ridge micropatterns. We also demonstrated that electrical stimulation improved the myoblast alignment and increased the diameter of the resulting myotubes. By using the microstructured methacrylated gelatin substrates, we built free-standing 3D muscle sheets, which contracted when electrically stimulated. Given their robust contractility and biomimetic microarchitecture, engineered tissues may find use in tissue engineering, biological studies, high-throughput drug screening, and biorobotics. |
| first_indexed | 2024-09-23T08:45:04Z |
| format | Article |
| id | mit-1721.1/77632 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:45:04Z |
| publishDate | 2013 |
| publisher | Mary Ann Liebert, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/776322022-09-23T14:17:43Z Engineered Contractile Skeletal Muscle Tissue on a Microgrooved Methacrylated Gelatin Substrate Hosseini, Vahid Ahadian, Samad Ostrovidov, Serge Camci-Unal, Gulden Chen, Song Kaji, Hirokazu Ramalingam, Murugan Khademhosseini, Ali Harvard University--MIT Division of Health Sciences and Technology Camci-Unal, Gulden To engineer tissue-like structures, cells must organize themselves into three-dimensional (3D) networks that mimic the native tissue microarchitecture. Microfabricated hydrogel substrates provide a potentially useful platform for directing cells into biomimetic tissue architecture in vitro. Here, we present microgrooved methacrylated gelatin hydrogels as a suitable platform to build muscle-like fibrous structures in a facile and highly reproducible fashion. Microgrooved hydrogel substrates with two different ridge sizes (50 and 100 μm) were fabricated to assess the effect of the distance between engineered myofibers on the orientation of the bridging C2C12 myoblasts and the formation of the resulting multinucleated myotubes. It was shown that although the ridge size did not significantly affect the C2C12 myoblast alignment, the wider-ridged micropatterned hydrogels generated more myotubes that were not aligned to the groove direction as compared to those on the smaller-ridge micropatterns. We also demonstrated that electrical stimulation improved the myoblast alignment and increased the diameter of the resulting myotubes. By using the microstructured methacrylated gelatin substrates, we built free-standing 3D muscle sheets, which contracted when electrically stimulated. Given their robust contractility and biomimetic microarchitecture, engineered tissues may find use in tissue engineering, biological studies, high-throughput drug screening, and biorobotics. 2013-03-12T18:37:56Z 2013-03-12T18:37:56Z 2012-09 2012-03 Article http://purl.org/eprint/type/JournalArticle 1937-3341 1937-335X http://hdl.handle.net/1721.1/77632 Hosseini, Vahid et al. “Engineered Contractile Skeletal Muscle Tissue on a Microgrooved Methacrylated Gelatin Substrate.” Tissue Engineering Part A 18.23-24 (2012): 2453–2465. en_US http://dx.doi.org/10.1089/ten.tea.2012.0181 Tissue Engineering Part A Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert, Inc. Mary Ann Leibert |
| spellingShingle | Hosseini, Vahid Ahadian, Samad Ostrovidov, Serge Camci-Unal, Gulden Chen, Song Kaji, Hirokazu Ramalingam, Murugan Khademhosseini, Ali Engineered Contractile Skeletal Muscle Tissue on a Microgrooved Methacrylated Gelatin Substrate |
| title | Engineered Contractile Skeletal Muscle Tissue on a Microgrooved Methacrylated Gelatin Substrate |
| title_full | Engineered Contractile Skeletal Muscle Tissue on a Microgrooved Methacrylated Gelatin Substrate |
| title_fullStr | Engineered Contractile Skeletal Muscle Tissue on a Microgrooved Methacrylated Gelatin Substrate |
| title_full_unstemmed | Engineered Contractile Skeletal Muscle Tissue on a Microgrooved Methacrylated Gelatin Substrate |
| title_short | Engineered Contractile Skeletal Muscle Tissue on a Microgrooved Methacrylated Gelatin Substrate |
| title_sort | engineered contractile skeletal muscle tissue on a microgrooved methacrylated gelatin substrate |
| url | http://hdl.handle.net/1721.1/77632 |
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