Peptide targeting and imaging of damaged lung tissue in influenza-infected mice
In this study, we investigate whether pH (low) insertion peptide (pHLIP) can target regions of lung injury associated with influenza infection. Materials & methods: Fluorophore-conjugated pHLIP was injected intraperitoneally into mice infected with a sublethal dose of H1N1 influenza and visualiz...
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| Format: | Article |
| Language: | en_US |
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Future Medicine
2013
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| Online Access: | http://hdl.handle.net/1721.1/77907 https://orcid.org/0000-0002-5687-6154 |
| _version_ | 1826191961330024448 |
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| author | Li, Na Yin, Lu Thevenin, Damien Yamada, Yoshiyuki Limmon, Gino V. Chen, Jianzhu Chow, Vincent T. K. Engelman, Donald M. Engelward, Bevin P. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Li, Na Yin, Lu Thevenin, Damien Yamada, Yoshiyuki Limmon, Gino V. Chen, Jianzhu Chow, Vincent T. K. Engelman, Donald M. Engelward, Bevin P. |
| author_sort | Li, Na |
| collection | MIT |
| description | In this study, we investigate whether pH (low) insertion peptide (pHLIP) can target regions of lung injury associated with influenza infection. Materials & methods: Fluorophore-conjugated pHLIP was injected intraperitoneally into mice infected with a sublethal dose of H1N1 influenza and visualized histologically. Results: pHLIP specifically targeted inflamed lung tissues of infected mice in the later stages of disease and at sites where alveolar type I and type II cells were depleted. Regions of pHLIP-targeted lung tissue were devoid of peroxiredoxin 6, the lung-abundant antioxidant enzyme, and were deficient in pneumocytes. Interestingly, a pHLIP variant possessing mutations that render it insensitive to pH changes was also able to target damaged lung tissue. Conclusion: pHLIP holds potential for delivering therapeutics for lung injury during influenza infection. Furthermore, there may be more than one mechanism that enables pHLIP variants to target inflamed lung tissue. |
| first_indexed | 2024-09-23T09:04:02Z |
| format | Article |
| id | mit-1721.1/77907 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:04:02Z |
| publishDate | 2013 |
| publisher | Future Medicine |
| record_format | dspace |
| spelling | mit-1721.1/779072022-09-30T13:12:29Z Peptide targeting and imaging of damaged lung tissue in influenza-infected mice Li, Na Yin, Lu Thevenin, Damien Yamada, Yoshiyuki Limmon, Gino V. Chen, Jianzhu Chow, Vincent T. K. Engelman, Donald M. Engelward, Bevin P. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Singapore-MIT Alliance in Research and Technology (SMART) Koch Institute for Integrative Cancer Research at MIT Engelward, Bevin P. Engelward, Bevin P. Li, Na Yin, Lu Yamada, Yoshiyuki Chen, Jianzhu Limmon, Gino V. In this study, we investigate whether pH (low) insertion peptide (pHLIP) can target regions of lung injury associated with influenza infection. Materials & methods: Fluorophore-conjugated pHLIP was injected intraperitoneally into mice infected with a sublethal dose of H1N1 influenza and visualized histologically. Results: pHLIP specifically targeted inflamed lung tissues of infected mice in the later stages of disease and at sites where alveolar type I and type II cells were depleted. Regions of pHLIP-targeted lung tissue were devoid of peroxiredoxin 6, the lung-abundant antioxidant enzyme, and were deficient in pneumocytes. Interestingly, a pHLIP variant possessing mutations that render it insensitive to pH changes was also able to target damaged lung tissue. Conclusion: pHLIP holds potential for delivering therapeutics for lung injury during influenza infection. Furthermore, there may be more than one mechanism that enables pHLIP variants to target inflamed lung tissue. National Institute of Environmental Health Sciences (P01 - ES006052) Singapore–MIT Alliance for Research and Technology National Institutes of Health (U.S.) (grant GM073857) 2013-03-15T16:16:51Z 2013-03-15T16:16:51Z 2013-02 Article http://purl.org/eprint/type/JournalArticle 1746-0913 1746-0921 http://hdl.handle.net/1721.1/77907 Li, Na et al. “Peptide Targeting and Imaging of Damaged Lung Tissue in Influenza-infected Mice.” Future Microbiology 8.2 (2013): 257–269. CrossRef. Web. https://orcid.org/0000-0002-5687-6154 en_US http://dx.doi.org/10.2217/fmb.12.134 Future Microbiology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Future Medicine Prof. Engelward via Howard |
| spellingShingle | Li, Na Yin, Lu Thevenin, Damien Yamada, Yoshiyuki Limmon, Gino V. Chen, Jianzhu Chow, Vincent T. K. Engelman, Donald M. Engelward, Bevin P. Peptide targeting and imaging of damaged lung tissue in influenza-infected mice |
| title | Peptide targeting and imaging of damaged lung tissue in influenza-infected mice |
| title_full | Peptide targeting and imaging of damaged lung tissue in influenza-infected mice |
| title_fullStr | Peptide targeting and imaging of damaged lung tissue in influenza-infected mice |
| title_full_unstemmed | Peptide targeting and imaging of damaged lung tissue in influenza-infected mice |
| title_short | Peptide targeting and imaging of damaged lung tissue in influenza-infected mice |
| title_sort | peptide targeting and imaging of damaged lung tissue in influenza infected mice |
| url | http://hdl.handle.net/1721.1/77907 https://orcid.org/0000-0002-5687-6154 |
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