Strain Release in Organic Photonic Nanoparticles for Protease Sensing
Proteases are overexpressed in most cancers and proteolytic activity has been shown to be a viable marker for cancer imaging in vivo. Herein, we describe the synthesis of luminescence-quenched shell cross-linked nanoparticles as photonic nanoprobes for protease sensing. Protease sensing scheme is ba...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Chemical Society
2013
|
| Online Access: | http://hdl.handle.net/1721.1/78310 |
| _version_ | 1826204208149299200 |
|---|---|
| author | Cordovilla, Carlos Swager, Timothy Manning |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Cordovilla, Carlos Swager, Timothy Manning |
| author_sort | Cordovilla, Carlos |
| collection | MIT |
| description | Proteases are overexpressed in most cancers and proteolytic activity has been shown to be a viable marker for cancer imaging in vivo. Herein, we describe the synthesis of luminescence-quenched shell cross-linked nanoparticles as photonic nanoprobes for protease sensing. Protease sensing scheme is based on a “turn-on” mechanism where the protease cleaves peptide cross-linkers of the fluorescence-quenched shell cross-linked NP (OFF state) leading to a highly emissive non-cross linked NP (ON state). The cross-linked particles can be strained by exposure to a good solvent and proteolysis allows for particle expansion (swelling) and a recovery of the luminescence. |
| first_indexed | 2024-09-23T12:50:36Z |
| format | Article |
| id | mit-1721.1/78310 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:50:36Z |
| publishDate | 2013 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | mit-1721.1/783102022-09-28T10:23:08Z Strain Release in Organic Photonic Nanoparticles for Protease Sensing Cordovilla, Carlos Swager, Timothy Manning Massachusetts Institute of Technology. Department of Chemistry Swager, Timothy Manning Swager, Timothy Manning Cordovilla, Carlos Proteases are overexpressed in most cancers and proteolytic activity has been shown to be a viable marker for cancer imaging in vivo. Herein, we describe the synthesis of luminescence-quenched shell cross-linked nanoparticles as photonic nanoprobes for protease sensing. Protease sensing scheme is based on a “turn-on” mechanism where the protease cleaves peptide cross-linkers of the fluorescence-quenched shell cross-linked NP (OFF state) leading to a highly emissive non-cross linked NP (ON state). The cross-linked particles can be strained by exposure to a good solvent and proteolysis allows for particle expansion (swelling) and a recovery of the luminescence. United States. Air Force Office of Scientific Research (FA9550-10-1-0395) Spain. Ministerio de Ciencia e Innovación (postdoctoral grant) 2013-04-09T20:51:53Z 2013-04-09T20:51:53Z 2012-04 2012-02 Article http://purl.org/eprint/type/JournalArticle 0002-7863 1520-5126 http://hdl.handle.net/1721.1/78310 Cordovilla, Carlos, and Timothy M. Swager. “Strain Release in Organic Photonic Nanoparticles for Protease Sensing.” Journal of the American Chemical Society 134.16 (2012): 6932–6935. CrossRef. Web. en_US http://dx.doi.org/10.1021/ja301259v Journal of the American Chemical Society Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society Prof. Swager via Erja Kajosalo |
| spellingShingle | Cordovilla, Carlos Swager, Timothy Manning Strain Release in Organic Photonic Nanoparticles for Protease Sensing |
| title | Strain Release in Organic Photonic Nanoparticles for Protease Sensing |
| title_full | Strain Release in Organic Photonic Nanoparticles for Protease Sensing |
| title_fullStr | Strain Release in Organic Photonic Nanoparticles for Protease Sensing |
| title_full_unstemmed | Strain Release in Organic Photonic Nanoparticles for Protease Sensing |
| title_short | Strain Release in Organic Photonic Nanoparticles for Protease Sensing |
| title_sort | strain release in organic photonic nanoparticles for protease sensing |
| url | http://hdl.handle.net/1721.1/78310 |
| work_keys_str_mv | AT cordovillacarlos strainreleaseinorganicphotonicnanoparticlesforproteasesensing AT swagertimothymanning strainreleaseinorganicphotonicnanoparticlesforproteasesensing |