Synoviocytes protect cartilage from the effects of injury in vitro
Background It is well documented that osteoarthritis (OA) can develop following traumatic joint injury and is the leading cause of lameness and subsequent wastage of equine athletes. Although much research of injury induced OA has focused on cartilage, OA is a disease that affects the whole joint...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
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BioMed Central Ltd.
2013
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| Online Access: | http://hdl.handle.net/1721.1/78328 https://orcid.org/0000-0002-4942-3456 |
| _version_ | 1826212303203205120 |
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| author | Lee, Christina M. Kisiday, John D. McIlwraith, C. Wayne Grodzinsky, Alan J. Frisbie, David D. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Lee, Christina M. Kisiday, John D. McIlwraith, C. Wayne Grodzinsky, Alan J. Frisbie, David D. |
| author_sort | Lee, Christina M. |
| collection | MIT |
| description | Background
It is well documented that osteoarthritis (OA) can develop following traumatic joint injury and is the leading cause of lameness and subsequent wastage of equine athletes. Although much research of injury induced OA has focused on cartilage, OA is a disease that affects the whole joint organ.
Methods
In this study, we investigated the impact of synovial cells on the progression of an OA phenotype in injured articular cartilage. Injured and control cartilage were cultured in the presence of synoviocytes extracted from normal equine synovium. Synoviocytes and cartilage were evaluated for catabolic and anabolic gene expression. The cartilage was also evaluated histologically for loss of extracellular matrix molecules, chondrocyte cell death and chondrocyte cluster formation.
Results
The results indicate synoviocytes exert both positive and negative effects on injured cartilage, but ultimately protect injured cartilage from progressing toward an OA phenotype. Synoviocytes cultured in the presence of injured cartilage had significantly reduced expression of aggrecanase 1 and 2 (ADAMTS4 and 5), but also had increased expression of matrix metalloproteinase (MMP) -1 and reduced expression of tissue inhibitor of metalloproteinases 1 (TIMP-1). Injured cartilage cultured with synoviocytes had increased expression of both collagen type 2 and aggrecanase 2. Histologic examination of cartilage indicated that there was a protective effect of synoviocytes on injured cartilage by reducing the incidence of both focal cell loss and chondrocyte cluster formation, two major hallmarks of OA.
Conclusions
These results support the importance of evaluating more than one synovial joint tissue when investigating injury induced OA. |
| first_indexed | 2024-09-23T15:19:34Z |
| format | Article |
| id | mit-1721.1/78328 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T15:19:34Z |
| publishDate | 2013 |
| publisher | BioMed Central Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/783282022-09-29T14:13:16Z Synoviocytes protect cartilage from the effects of injury in vitro Lee, Christina M. Kisiday, John D. McIlwraith, C. Wayne Grodzinsky, Alan J. Frisbie, David D. Massachusetts Institute of Technology. Department of Biological Engineering Grodzinsky, Alan J. Background It is well documented that osteoarthritis (OA) can develop following traumatic joint injury and is the leading cause of lameness and subsequent wastage of equine athletes. Although much research of injury induced OA has focused on cartilage, OA is a disease that affects the whole joint organ. Methods In this study, we investigated the impact of synovial cells on the progression of an OA phenotype in injured articular cartilage. Injured and control cartilage were cultured in the presence of synoviocytes extracted from normal equine synovium. Synoviocytes and cartilage were evaluated for catabolic and anabolic gene expression. The cartilage was also evaluated histologically for loss of extracellular matrix molecules, chondrocyte cell death and chondrocyte cluster formation. Results The results indicate synoviocytes exert both positive and negative effects on injured cartilage, but ultimately protect injured cartilage from progressing toward an OA phenotype. Synoviocytes cultured in the presence of injured cartilage had significantly reduced expression of aggrecanase 1 and 2 (ADAMTS4 and 5), but also had increased expression of matrix metalloproteinase (MMP) -1 and reduced expression of tissue inhibitor of metalloproteinases 1 (TIMP-1). Injured cartilage cultured with synoviocytes had increased expression of both collagen type 2 and aggrecanase 2. Histologic examination of cartilage indicated that there was a protective effect of synoviocytes on injured cartilage by reducing the incidence of both focal cell loss and chondrocyte cluster formation, two major hallmarks of OA. Conclusions These results support the importance of evaluating more than one synovial joint tissue when investigating injury induced OA. National Institutes of Health (U.S.) (Grant AR60331) 2013-04-10T16:09:26Z 2013-04-10T16:09:26Z 2013-02 2012-04 2013-04-08T11:07:23Z Article http://purl.org/eprint/type/JournalArticle 1471-2474 http://hdl.handle.net/1721.1/78328 Lee, Christina M et al. “Synoviocytes Protect Cartilage from the Effects of Injury in Vitro.” BMC Musculoskeletal Disorders 14.1 (2013): 54. CrossRef. Web. https://orcid.org/0000-0002-4942-3456 en http://dx.doi.org/10.1186/1471-2474-14-54 BMC Musculoskeletal Disorders Creative Commons Attribution http://creativecommons.org/licenses/by/2.0 Christina M Lee et al.; licensee BioMed Central Ltd. application/pdf BioMed Central Ltd. BioMed Central Ltd |
| spellingShingle | Lee, Christina M. Kisiday, John D. McIlwraith, C. Wayne Grodzinsky, Alan J. Frisbie, David D. Synoviocytes protect cartilage from the effects of injury in vitro |
| title | Synoviocytes protect cartilage from the effects of injury in vitro |
| title_full | Synoviocytes protect cartilage from the effects of injury in vitro |
| title_fullStr | Synoviocytes protect cartilage from the effects of injury in vitro |
| title_full_unstemmed | Synoviocytes protect cartilage from the effects of injury in vitro |
| title_short | Synoviocytes protect cartilage from the effects of injury in vitro |
| title_sort | synoviocytes protect cartilage from the effects of injury in vitro |
| url | http://hdl.handle.net/1721.1/78328 https://orcid.org/0000-0002-4942-3456 |
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