Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography
We demonstrate the use of a high resolution form of optical coherence tomography, termed micro-OCT (μOCT), for investigating the functional microanatomy of airway epithelia. μOCT captures several key parameters governing the function of the airway surface (airway surface liquid depth, periciliary li...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2013
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| Online Access: | http://hdl.handle.net/1721.1/78568 https://orcid.org/0000-0002-3402-6634 |
| _version_ | 1826190190066008064 |
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| author | Diephuis, Bradford J. Tearney, Guillermo J. Liu, Linbo Chu, Kengyeh K. Houser, Grace H. Li, Yao Wilsterman, Eric J. Shastry, Suresh Dierksen, Gregory Birket, Susan E. Birket, Susan E. Byan-Parker, Suzanne Grizzle, William E. Sorscher, Eric J. Rowe, Steven M. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Diephuis, Bradford J. Tearney, Guillermo J. Liu, Linbo Chu, Kengyeh K. Houser, Grace H. Li, Yao Wilsterman, Eric J. Shastry, Suresh Dierksen, Gregory Birket, Susan E. Birket, Susan E. Byan-Parker, Suzanne Grizzle, William E. Sorscher, Eric J. Rowe, Steven M. |
| author_sort | Diephuis, Bradford J. |
| collection | MIT |
| description | We demonstrate the use of a high resolution form of optical coherence tomography, termed micro-OCT (μOCT), for investigating the functional microanatomy of airway epithelia. μOCT captures several key parameters governing the function of the airway surface (airway surface liquid depth, periciliary liquid depth, ciliary function including beat frequency, and mucociliary transport rate) from the same series of images and without exogenous particles or labels, enabling non-invasive study of dynamic phenomena. Additionally, the high resolution of μOCT reveals distinguishable phases of the ciliary stroke pattern and glandular extrusion. Images and functional measurements from primary human bronchial epithelial cell cultures and excised tissue are presented and compared with measurements using existing gold standard methods. Active secretion from mucus glands in tissue, a key parameter of epithelial function, was also observed and quantified. |
| first_indexed | 2024-09-23T08:36:27Z |
| format | Article |
| id | mit-1721.1/78568 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:36:27Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/785682022-09-30T09:55:09Z Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography Diephuis, Bradford J. Tearney, Guillermo J. Liu, Linbo Chu, Kengyeh K. Houser, Grace H. Li, Yao Wilsterman, Eric J. Shastry, Suresh Dierksen, Gregory Birket, Susan E. Birket, Susan E. Byan-Parker, Suzanne Grizzle, William E. Sorscher, Eric J. Rowe, Steven M. Harvard University--MIT Division of Health Sciences and Technology Diephuis, Bradford J. Tearney, Guillermo J. We demonstrate the use of a high resolution form of optical coherence tomography, termed micro-OCT (μOCT), for investigating the functional microanatomy of airway epithelia. μOCT captures several key parameters governing the function of the airway surface (airway surface liquid depth, periciliary liquid depth, ciliary function including beat frequency, and mucociliary transport rate) from the same series of images and without exogenous particles or labels, enabling non-invasive study of dynamic phenomena. Additionally, the high resolution of μOCT reveals distinguishable phases of the ciliary stroke pattern and glandular extrusion. Images and functional measurements from primary human bronchial epithelial cell cultures and excised tissue are presented and compared with measurements using existing gold standard methods. Active secretion from mucus glands in tissue, a key parameter of epithelial function, was also observed and quantified. 2013-04-17T16:53:04Z 2013-04-17T16:53:04Z 2013-01 2012-10 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/78568 Liu, Linbo et al. “Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography.” Ed. Michael Myerburg. PLoS ONE 8.1 (2013): e54473. https://orcid.org/0000-0002-3402-6634 en_US http://dx.doi.org/10.1371/journal.pone.0054473 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Diephuis, Bradford J. Tearney, Guillermo J. Liu, Linbo Chu, Kengyeh K. Houser, Grace H. Li, Yao Wilsterman, Eric J. Shastry, Suresh Dierksen, Gregory Birket, Susan E. Birket, Susan E. Byan-Parker, Suzanne Grizzle, William E. Sorscher, Eric J. Rowe, Steven M. Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography |
| title | Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography |
| title_full | Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography |
| title_fullStr | Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography |
| title_full_unstemmed | Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography |
| title_short | Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography |
| title_sort | method for quantitative study of airway functional microanatomy using micro optical coherence tomography |
| url | http://hdl.handle.net/1721.1/78568 https://orcid.org/0000-0002-3402-6634 |
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