Quantitative polarized light microscopy of unstained mammalian cochlear sections
Hearing loss is the most common sensory deficit in the world, and most frequently it originates in the inner ear. Yet, the inner ear has been difficult to access for diagnosis because of its small size, delicate nature, complex three-dimensional anatomy, and encasement in the densest bone in the bod...
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| Format: | Article |
| Language: | en_US |
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SPIE
2013
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| Online Access: | http://hdl.handle.net/1721.1/78585 https://orcid.org/0000-0003-0233-279X https://orcid.org/0000-0001-8323-2779 |
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| author | Kalwani, Neil M. Ong, Cheng Ai Lysaght, Andrew Christopher Stankovic, Konstantina M. Haward, Simon J. McKinley, Gareth H. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Kalwani, Neil M. Ong, Cheng Ai Lysaght, Andrew Christopher Stankovic, Konstantina M. Haward, Simon J. McKinley, Gareth H. |
| author_sort | Kalwani, Neil M. |
| collection | MIT |
| description | Hearing loss is the most common sensory deficit in the world, and most frequently it originates in the inner ear. Yet, the inner ear has been difficult to access for diagnosis because of its small size, delicate nature, complex three-dimensional anatomy, and encasement in the densest bone in the body. Evolving optical methods are promising to afford cellular diagnosis of pathologic changes in the inner ear. To appropriately interpret results from these emerging technologies, it is important to characterize optical properties of cochlear tissues. Here, we focus on that characterization using quantitative polarized light microscopy (qPLM) applied to unstained cochlear sections of the mouse, a common animal model of human hearing loss. We find that the most birefringent cochlear materials are collagen fibrils and myelin. Retardance of the otic capsule, the spiral ligament, and the basilar membrane are substantially higher than that of other cochlear structures. Retardance of the spiral ligament and the basilar membrane decrease from the cochlear base to the apex, compared with the more uniform retardance of other structures. The intricate structural details revealed by qPLM of unstained cochlear sections ex vivo strongly motivate future application of polarization-sensitive optical coherence tomography to human cochlea in vivo. |
| first_indexed | 2024-09-23T09:41:42Z |
| format | Article |
| id | mit-1721.1/78585 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:41:42Z |
| publishDate | 2013 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/785852022-09-26T13:11:12Z Quantitative polarized light microscopy of unstained mammalian cochlear sections Kalwani, Neil M. Ong, Cheng Ai Lysaght, Andrew Christopher Stankovic, Konstantina M. Haward, Simon J. McKinley, Gareth H. Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Mechanical Engineering Lysaght, Andrew Christopher Stankovic, Konstantina M. Haward, Simon J. McKinley, Gareth H. Hearing loss is the most common sensory deficit in the world, and most frequently it originates in the inner ear. Yet, the inner ear has been difficult to access for diagnosis because of its small size, delicate nature, complex three-dimensional anatomy, and encasement in the densest bone in the body. Evolving optical methods are promising to afford cellular diagnosis of pathologic changes in the inner ear. To appropriately interpret results from these emerging technologies, it is important to characterize optical properties of cochlear tissues. Here, we focus on that characterization using quantitative polarized light microscopy (qPLM) applied to unstained cochlear sections of the mouse, a common animal model of human hearing loss. We find that the most birefringent cochlear materials are collagen fibrils and myelin. Retardance of the otic capsule, the spiral ligament, and the basilar membrane are substantially higher than that of other cochlear structures. Retardance of the spiral ligament and the basilar membrane decrease from the cochlear base to the apex, compared with the more uniform retardance of other structures. The intricate structural details revealed by qPLM of unstained cochlear sections ex vivo strongly motivate future application of polarization-sensitive optical coherence tomography to human cochlea in vivo. United States. National Institute for Deafness and other Communicative Disorders (Grant K08 DC010419) United States. National Institute for Deafness and other Communicative Disorders (Grant United States. National Institute for Deafness and other Communicative Disorders (Grant K08 DC010419)) NASA Microgravity Fluid Sciences (Grant NNX09AV99G) 2013-04-24T15:10:07Z 2013-04-24T15:10:07Z 2013-02 2013-01 Article http://purl.org/eprint/type/JournalArticle 1083-3668 1560-2281 http://hdl.handle.net/1721.1/78585 Kalwani, Neil M. “Quantitative Polarized Light Microscopy of Unstained Mammalian Cochlear Sections.” Journal of Biomedical Optics 18.2 (2013): 026021. ©2013 Society of Photo-Optical Instrumentation Engineers https://orcid.org/0000-0003-0233-279X https://orcid.org/0000-0001-8323-2779 en_US http://dx.doi.org/10.1117/1.jbo.18.2.026021 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Kalwani, Neil M. Ong, Cheng Ai Lysaght, Andrew Christopher Stankovic, Konstantina M. Haward, Simon J. McKinley, Gareth H. Quantitative polarized light microscopy of unstained mammalian cochlear sections |
| title | Quantitative polarized light microscopy of unstained mammalian cochlear sections |
| title_full | Quantitative polarized light microscopy of unstained mammalian cochlear sections |
| title_fullStr | Quantitative polarized light microscopy of unstained mammalian cochlear sections |
| title_full_unstemmed | Quantitative polarized light microscopy of unstained mammalian cochlear sections |
| title_short | Quantitative polarized light microscopy of unstained mammalian cochlear sections |
| title_sort | quantitative polarized light microscopy of unstained mammalian cochlear sections |
| url | http://hdl.handle.net/1721.1/78585 https://orcid.org/0000-0003-0233-279X https://orcid.org/0000-0001-8323-2779 |
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