Aneuploid yeast strains exhibit defects in cell growth and passage through START
Aneuploidy, a chromosome content that is not a multiple of the haploid karyotype, is associated with reduced fitness in all organisms analyzed to date. In budding yeast aneuploidy causes cell proliferation defects, with many different aneuploid strains exhibiting a delay in G1, a cell cycle stage go...
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| Format: | Article |
| Language: | en_US |
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American Society for Cell Biology
2013
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| Online Access: | http://hdl.handle.net/1721.1/78586 https://orcid.org/0000-0002-7699-9354 https://orcid.org/0000-0002-2345-5038 https://orcid.org/0000-0001-9837-0314 |
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| author | Thorburn, Rebecca Ruth Gonzalez, Christian Brar, Gloria A. Christen, Stefan Carlile, Thomas M. Ingolia, Nicholas T. Sauer, Uwe Weissman, Jonathan S. Amon, Angelika B |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Thorburn, Rebecca Ruth Gonzalez, Christian Brar, Gloria A. Christen, Stefan Carlile, Thomas M. Ingolia, Nicholas T. Sauer, Uwe Weissman, Jonathan S. Amon, Angelika B |
| author_sort | Thorburn, Rebecca Ruth |
| collection | MIT |
| description | Aneuploidy, a chromosome content that is not a multiple of the haploid karyotype, is associated with reduced fitness in all organisms analyzed to date. In budding yeast aneuploidy causes cell proliferation defects, with many different aneuploid strains exhibiting a delay in G1, a cell cycle stage governed by extracellular cues, growth rate and cell cycle events. Here, we characterize this G1 delay. We show that 10 of 14 aneuploid yeast strains exhibit a growth defect during G1. Furthermore, 10 of 14 aneuploid strains display a cell cycle entry delay that correlates with the size of the additional chromosome. This cell cycle entry delay is due to a delayed accumulation of G1 cyclins that can be suppressed by supplying cells with high levels of a G1 cyclin. Our results indicate that aneuploidy frequently interferes with the ability of cells to grow and, as many other cellular stresses, entry into the cell cycle. |
| first_indexed | 2024-09-23T12:19:23Z |
| format | Article |
| id | mit-1721.1/78586 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:19:23Z |
| publishDate | 2013 |
| publisher | American Society for Cell Biology |
| record_format | dspace |
| spelling | mit-1721.1/785862022-09-28T01:01:13Z Aneuploid yeast strains exhibit defects in cell growth and passage through START Thorburn, Rebecca Ruth Gonzalez, Christian Brar, Gloria A. Christen, Stefan Carlile, Thomas M. Ingolia, Nicholas T. Sauer, Uwe Weissman, Jonathan S. Amon, Angelika B Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Thorburn, Rebecca Ruth Gonzalez, Christian Carlile, Thomas M. Amon, Angelika B. Aneuploidy, a chromosome content that is not a multiple of the haploid karyotype, is associated with reduced fitness in all organisms analyzed to date. In budding yeast aneuploidy causes cell proliferation defects, with many different aneuploid strains exhibiting a delay in G1, a cell cycle stage governed by extracellular cues, growth rate and cell cycle events. Here, we characterize this G1 delay. We show that 10 of 14 aneuploid yeast strains exhibit a growth defect during G1. Furthermore, 10 of 14 aneuploid strains display a cell cycle entry delay that correlates with the size of the additional chromosome. This cell cycle entry delay is due to a delayed accumulation of G1 cyclins that can be suppressed by supplying cells with high levels of a G1 cyclin. Our results indicate that aneuploidy frequently interferes with the ability of cells to grow and, as many other cellular stresses, entry into the cell cycle. National Institutes of Health (GM056800) 2013-04-24T15:29:41Z 2013-04-24T15:29:41Z 2013-03 2013-02 Article http://purl.org/eprint/type/JournalArticle 1059-1524 1939-4586 http://hdl.handle.net/1721.1/78586 Thorburn, R. R. et al. “Aneuploid Yeast Strains Exhibit Defects in Cell Growth and Passage Through START.” Molecular Biology of the Cell (2013). ©2013 by The American Society for Cell Biology https://orcid.org/0000-0002-7699-9354 https://orcid.org/0000-0002-2345-5038 https://orcid.org/0000-0001-9837-0314 en_US http://dx.doi.org/10.1091/mbc.E12-07-0520 Molecular Biology of the Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Society for Cell Biology American Society for Cell Biology |
| spellingShingle | Thorburn, Rebecca Ruth Gonzalez, Christian Brar, Gloria A. Christen, Stefan Carlile, Thomas M. Ingolia, Nicholas T. Sauer, Uwe Weissman, Jonathan S. Amon, Angelika B Aneuploid yeast strains exhibit defects in cell growth and passage through START |
| title | Aneuploid yeast strains exhibit defects in cell growth and passage through START |
| title_full | Aneuploid yeast strains exhibit defects in cell growth and passage through START |
| title_fullStr | Aneuploid yeast strains exhibit defects in cell growth and passage through START |
| title_full_unstemmed | Aneuploid yeast strains exhibit defects in cell growth and passage through START |
| title_short | Aneuploid yeast strains exhibit defects in cell growth and passage through START |
| title_sort | aneuploid yeast strains exhibit defects in cell growth and passage through start |
| url | http://hdl.handle.net/1721.1/78586 https://orcid.org/0000-0002-7699-9354 https://orcid.org/0000-0002-2345-5038 https://orcid.org/0000-0001-9837-0314 |
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