Implantable microenvironments to attract hematopoietic stem/cancer cells
The environments that harbor hematopoietic stem and progenitor cells are critical to explore for a better understanding of hematopoiesis during health and disease. These compartments often are inaccessible for controlled and rapid experimentation, thus limiting studies to the evaluation of conventio...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2013
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| Online Access: | http://hdl.handle.net/1721.1/78835 https://orcid.org/0000-0001-9296-2220 |
| _version_ | 1826194354534875136 |
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| author | Lee, Jungwoo Li, Matthew Milwid, Jack Dunham, Joshua Vinegoni, Claudio Gorbatov, Rostic Iwamoto, Yoshiko Wang, Fangjing Shen, Keyue Hatfield, Kimberley Enger, Marianne Shafiee, Sahba McCormack, Emmet Ebert, Benjamin L. Weissleder, Ralph Yarmush, Martin L. Parekkadan, Biju |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Lee, Jungwoo Li, Matthew Milwid, Jack Dunham, Joshua Vinegoni, Claudio Gorbatov, Rostic Iwamoto, Yoshiko Wang, Fangjing Shen, Keyue Hatfield, Kimberley Enger, Marianne Shafiee, Sahba McCormack, Emmet Ebert, Benjamin L. Weissleder, Ralph Yarmush, Martin L. Parekkadan, Biju |
| author_sort | Lee, Jungwoo |
| collection | MIT |
| description | The environments that harbor hematopoietic stem and progenitor cells are critical to explore for a better understanding of hematopoiesis during health and disease. These compartments often are inaccessible for controlled and rapid experimentation, thus limiting studies to the evaluation of conventional cell culture and transgenic animal models. Here we describe the manufacture and image-guided monitoring of an engineered microenvironment with user-defined properties that recruits hematopoietic progenitors into the implant. Using intravital imaging and fluorescence molecular tomography, we show in real time that the cell homing and retention process is efficient and durable for short- and long-term engraftment studies. Our results indicate that bone marrow stromal cells, precoated on the implant, accelerate the formation of new sinusoidal blood vessels with vascular integrity at the microcapillary level that enhances the recruitment hematopoietic progenitor cells to the site. This implantable construct can serve as a tool enabling the study of hematopoiesis. |
| first_indexed | 2024-09-23T09:54:25Z |
| format | Article |
| id | mit-1721.1/78835 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:54:25Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/788352022-09-30T17:37:59Z Implantable microenvironments to attract hematopoietic stem/cancer cells Lee, Jungwoo Li, Matthew Milwid, Jack Dunham, Joshua Vinegoni, Claudio Gorbatov, Rostic Iwamoto, Yoshiko Wang, Fangjing Shen, Keyue Hatfield, Kimberley Enger, Marianne Shafiee, Sahba McCormack, Emmet Ebert, Benjamin L. Weissleder, Ralph Yarmush, Martin L. Parekkadan, Biju Harvard University--MIT Division of Health Sciences and Technology Li, Matthew Milwid, Jack The environments that harbor hematopoietic stem and progenitor cells are critical to explore for a better understanding of hematopoiesis during health and disease. These compartments often are inaccessible for controlled and rapid experimentation, thus limiting studies to the evaluation of conventional cell culture and transgenic animal models. Here we describe the manufacture and image-guided monitoring of an engineered microenvironment with user-defined properties that recruits hematopoietic progenitors into the implant. Using intravital imaging and fluorescence molecular tomography, we show in real time that the cell homing and retention process is efficient and durable for short- and long-term engraftment studies. Our results indicate that bone marrow stromal cells, precoated on the implant, accelerate the formation of new sinusoidal blood vessels with vascular integrity at the microcapillary level that enhances the recruitment hematopoietic progenitor cells to the site. This implantable construct can serve as a tool enabling the study of hematopoiesis. National Institutes of Health (U.S.) (Grant R01EB012521) National Institutes of Health (U.S.) (Grant K01DK087770) National Cancer Institute (U.S.) (Grant 1K99CA163671-01A1) Shriners Hospital for Children (Postdoctoral Fellowship) 2013-05-07T15:32:13Z 2013-05-07T15:32:13Z 2012-11 2012-05 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/78835 Lee, J. et al. “Implantable Microenvironments to Attract Hematopoietic Stem/cancer Cells.” Proceedings of the National Academy of Sciences 109.48 (2012): 19638–19643. https://orcid.org/0000-0001-9296-2220 en_US http://dx.doi.org/10.1073/pnas.1208384109 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Public Library of Science PNAS |
| spellingShingle | Lee, Jungwoo Li, Matthew Milwid, Jack Dunham, Joshua Vinegoni, Claudio Gorbatov, Rostic Iwamoto, Yoshiko Wang, Fangjing Shen, Keyue Hatfield, Kimberley Enger, Marianne Shafiee, Sahba McCormack, Emmet Ebert, Benjamin L. Weissleder, Ralph Yarmush, Martin L. Parekkadan, Biju Implantable microenvironments to attract hematopoietic stem/cancer cells |
| title | Implantable microenvironments to attract hematopoietic stem/cancer cells |
| title_full | Implantable microenvironments to attract hematopoietic stem/cancer cells |
| title_fullStr | Implantable microenvironments to attract hematopoietic stem/cancer cells |
| title_full_unstemmed | Implantable microenvironments to attract hematopoietic stem/cancer cells |
| title_short | Implantable microenvironments to attract hematopoietic stem/cancer cells |
| title_sort | implantable microenvironments to attract hematopoietic stem cancer cells |
| url | http://hdl.handle.net/1721.1/78835 https://orcid.org/0000-0001-9296-2220 |
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