Genomes of marine cyanopodoviruses reveal multiple origins of diversity
The marine cyanobacteria Prochlorococcus and Synechococcus are highly abundant in the global oceans, as are the cyanophage with which they co-evolve. While genomic analyses have been relatively extensive for cyanomyoviruses, only three cyanopodoviruses isolated on marine cyanobacteria have been sequ...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2013
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| Online Access: | http://hdl.handle.net/1721.1/78852 https://orcid.org/0000-0003-1072-6828 https://orcid.org/0000-0002-4243-0418 |
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| author | Labrie, Simon J. Frois-Moniz, Katya Osburne, Marcia Kelly, Libusha Roggensack, Sara Sullivan, M. B. Gearin, Gary Zeng, Q. Fitzgerald, M. Henn, Matthew R. Chisholm, Sallie (Penny) |
| author2 | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Labrie, Simon J. Frois-Moniz, Katya Osburne, Marcia Kelly, Libusha Roggensack, Sara Sullivan, M. B. Gearin, Gary Zeng, Q. Fitzgerald, M. Henn, Matthew R. Chisholm, Sallie (Penny) |
| author_sort | Labrie, Simon J. |
| collection | MIT |
| description | The marine cyanobacteria Prochlorococcus and Synechococcus are highly abundant in the global oceans, as are the cyanophage with which they co-evolve. While genomic analyses have been relatively extensive for cyanomyoviruses, only three cyanopodoviruses isolated on marine cyanobacteria have been sequenced. Here we present nine new cyanopodovirus genomes, and analyse them in the context of the broader group. The genomes range from 42.2 to 47.7 kb, with G+C contents consistent with those of their hosts. They share 12 core genes, and the pan-genome is not close to being fully sampled. The genomes contain three variable island regions, with the most hypervariable genes concentrated at one end of the genome. Concatenated core-gene phylogeny clusters all but one of the phage into three distinct groups (MPP-A and two discrete clades within MPP-B). The outlier, P-RSP2, has the smallest genome and lacks RNA polymerase, a hallmark of the Autographivirinae subfamily. The phage in group MPP-B contain photosynthesis and carbon metabolism associated genes, while group MPP-A and the outlier P-RSP2 do not, suggesting different constraints on their lytic cycles. Four of the phage encode integrases and three have a host integration signature. Metagenomic analyses reveal that cyanopodoviruses may be more abundant in the oceans than previously thought. |
| first_indexed | 2024-09-23T08:01:42Z |
| format | Article |
| id | mit-1721.1/78852 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:01:42Z |
| publishDate | 2013 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/788522022-09-30T01:47:26Z Genomes of marine cyanopodoviruses reveal multiple origins of diversity Labrie, Simon J. Frois-Moniz, Katya Osburne, Marcia Kelly, Libusha Roggensack, Sara Sullivan, M. B. Gearin, Gary Zeng, Q. Fitzgerald, M. Henn, Matthew R. Chisholm, Sallie (Penny) Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Labrie, Simon J. Labrie, Simon J. Frois-Moniz, Katya Osburne, Marcia Kelly, Libusha Roggensack, Sara Sullivan, M. B. The marine cyanobacteria Prochlorococcus and Synechococcus are highly abundant in the global oceans, as are the cyanophage with which they co-evolve. While genomic analyses have been relatively extensive for cyanomyoviruses, only three cyanopodoviruses isolated on marine cyanobacteria have been sequenced. Here we present nine new cyanopodovirus genomes, and analyse them in the context of the broader group. The genomes range from 42.2 to 47.7 kb, with G+C contents consistent with those of their hosts. They share 12 core genes, and the pan-genome is not close to being fully sampled. The genomes contain three variable island regions, with the most hypervariable genes concentrated at one end of the genome. Concatenated core-gene phylogeny clusters all but one of the phage into three distinct groups (MPP-A and two discrete clades within MPP-B). The outlier, P-RSP2, has the smallest genome and lacks RNA polymerase, a hallmark of the Autographivirinae subfamily. The phage in group MPP-B contain photosynthesis and carbon metabolism associated genes, while group MPP-A and the outlier P-RSP2 do not, suggesting different constraints on their lytic cycles. Four of the phage encode integrases and three have a host integration signature. Metagenomic analyses reveal that cyanopodoviruses may be more abundant in the oceans than previously thought. National Science Foundation (U.S.). Center for Microbial Oceanography: Research and Education (Grant OCE-042560) National Science Foundation (U.S.). Center for Microbial Oceanography: Research and Education (Grant EF 0424599) 2013-05-09T18:21:15Z 2013-05-09T18:21:15Z 2013-01 2012-10 Article http://purl.org/eprint/type/JournalArticle 1462-2912 1462-2920 http://hdl.handle.net/1721.1/78852 Labrie, S. J. et al. “Genomes of Marine Cyanopodoviruses Reveal Multiple Origins of Diversity.” Environmental Microbiology 15.5 (2013): 1356–1376. https://orcid.org/0000-0003-1072-6828 https://orcid.org/0000-0002-4243-0418 en_US http://dx.doi.org/10.1111/1462-2920.12053 Environmental Microbiology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Wiley Blackwell Simon Labrie |
| spellingShingle | Labrie, Simon J. Frois-Moniz, Katya Osburne, Marcia Kelly, Libusha Roggensack, Sara Sullivan, M. B. Gearin, Gary Zeng, Q. Fitzgerald, M. Henn, Matthew R. Chisholm, Sallie (Penny) Genomes of marine cyanopodoviruses reveal multiple origins of diversity |
| title | Genomes of marine cyanopodoviruses reveal multiple origins of diversity |
| title_full | Genomes of marine cyanopodoviruses reveal multiple origins of diversity |
| title_fullStr | Genomes of marine cyanopodoviruses reveal multiple origins of diversity |
| title_full_unstemmed | Genomes of marine cyanopodoviruses reveal multiple origins of diversity |
| title_short | Genomes of marine cyanopodoviruses reveal multiple origins of diversity |
| title_sort | genomes of marine cyanopodoviruses reveal multiple origins of diversity |
| url | http://hdl.handle.net/1721.1/78852 https://orcid.org/0000-0003-1072-6828 https://orcid.org/0000-0002-4243-0418 |
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