Cell-surface sensors for real-time probing of cellular environments
Author Manuscript 2012 August 1.
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2013
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| Online Access: | http://hdl.handle.net/1721.1/78868 https://orcid.org/0000-0003-0588-9286 https://orcid.org/0000-0002-5921-3436 https://orcid.org/0000-0002-0160-8742 https://orcid.org/0000-0003-0921-3144 |
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| author | Zhao, Weian Schafer, Sebastian Choi, Jonghoon Yamanaka, Yvonne Joy Lombardi, Maria L. Bose, Suman Carlson, Alicia L. Phillips, Joseph A. Teo, Weisuong Droujinine, Ilia A. Cui, Cheryl Jain, Rakesh K. Lammerding, Jan Love, J. Christopher Lin, Charles Sarkar, Debanjan Karnik, Rohit Karp, Jeffrey Michael |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Zhao, Weian Schafer, Sebastian Choi, Jonghoon Yamanaka, Yvonne Joy Lombardi, Maria L. Bose, Suman Carlson, Alicia L. Phillips, Joseph A. Teo, Weisuong Droujinine, Ilia A. Cui, Cheryl Jain, Rakesh K. Lammerding, Jan Love, J. Christopher Lin, Charles Sarkar, Debanjan Karnik, Rohit Karp, Jeffrey Michael |
| author_sort | Zhao, Weian |
| collection | MIT |
| description | Author Manuscript 2012 August 1. |
| first_indexed | 2024-09-23T15:56:52Z |
| format | Article |
| id | mit-1721.1/78868 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:56:52Z |
| publishDate | 2013 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/788682022-09-29T17:13:11Z Cell-surface sensors for real-time probing of cellular environments Zhao, Weian Schafer, Sebastian Choi, Jonghoon Yamanaka, Yvonne Joy Lombardi, Maria L. Bose, Suman Carlson, Alicia L. Phillips, Joseph A. Teo, Weisuong Droujinine, Ilia A. Cui, Cheryl Jain, Rakesh K. Lammerding, Jan Love, J. Christopher Lin, Charles Sarkar, Debanjan Karnik, Rohit Karp, Jeffrey Michael Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Zhao, Weian Schafer, Sebastian Choi, Jonghoon Yamanaka, Yvonne Joy Bose, Suman Phillips, Joseph A. Teo, Weisuong Droujinine, Ilia A. Cui, Cheryl Love, J. Christopher Sarkar, Debanjan Karnik, Rohit Karp, Jeffrey Michael Author Manuscript 2012 August 1. The ability to explore cell signalling and cell-to-cell communication is essential for understanding cell biology and developing effective therapeutics. However, it is not yet possible to monitor the interaction of cells with their environments in real time. Here, we show that a fluorescent sensor attached to a cell membrane can detect signalling molecules in the cellular environment. The sensor is an aptamer (a short length of single-stranded DNA) that binds to platelet-derived growth factor (PDGF) and contains a pair of fluorescent dyes. When bound to PDGF, the aptamer changes conformation and the dyes come closer to each other, producing a signal. The sensor, which is covalently attached to the membranes of mesenchymal stem cells, can quantitatively detect with high spatial and temporal resolution PDGF that is added in cell culture medium or secreted by neighbouring cells. The engineered stem cells retain their ability to find their way to the bone marrow and can be monitored in vivo at the single-cell level using intravital microscopy. National Institutes of Health (U.S.) (Grant HL097172) National Institutes of Health (U.S.) (Grant HL095722) National Institutes of Health (U.S.) (Grant DE019191) National Institutes of Health (U.S.) (Grant NIAID 5RC1AI086152) Charles A. Dana Foundation American Heart Association (Grant 0970178N) National Science Foundation (U.S.) (Graduate Fellowship) 2013-05-13T20:54:09Z 2013-05-13T20:54:09Z 2011-07 2010-12 Article http://purl.org/eprint/type/JournalArticle 1748-3387 1748-3395 http://hdl.handle.net/1721.1/78868 Zhao, Weian, Sebastian Schafer, Jonghoon Choi, et al. 2011Cell-surface Sensors for Real-time Probing of Cellular Environments. Nature Nanotechnology 6(8): 524–531. https://orcid.org/0000-0003-0588-9286 https://orcid.org/0000-0002-5921-3436 https://orcid.org/0000-0002-0160-8742 https://orcid.org/0000-0003-0921-3144 en_US http://dx.doi.org/10.1038/nnano.2011.101 Nature Nanotechnology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Nature Publishing Group PubMed Central |
| spellingShingle | Zhao, Weian Schafer, Sebastian Choi, Jonghoon Yamanaka, Yvonne Joy Lombardi, Maria L. Bose, Suman Carlson, Alicia L. Phillips, Joseph A. Teo, Weisuong Droujinine, Ilia A. Cui, Cheryl Jain, Rakesh K. Lammerding, Jan Love, J. Christopher Lin, Charles Sarkar, Debanjan Karnik, Rohit Karp, Jeffrey Michael Cell-surface sensors for real-time probing of cellular environments |
| title | Cell-surface sensors for real-time probing of cellular environments |
| title_full | Cell-surface sensors for real-time probing of cellular environments |
| title_fullStr | Cell-surface sensors for real-time probing of cellular environments |
| title_full_unstemmed | Cell-surface sensors for real-time probing of cellular environments |
| title_short | Cell-surface sensors for real-time probing of cellular environments |
| title_sort | cell surface sensors for real time probing of cellular environments |
| url | http://hdl.handle.net/1721.1/78868 https://orcid.org/0000-0003-0588-9286 https://orcid.org/0000-0002-5921-3436 https://orcid.org/0000-0002-0160-8742 https://orcid.org/0000-0003-0921-3144 |
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