Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements
Author Manuscript 2013 March 21.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Royal Society of Chemistry, The
2013
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| Online Access: | http://hdl.handle.net/1721.1/78877 https://orcid.org/0000-0003-0588-9286 |
| _version_ | 1826199723792400384 |
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| author | Sen, Yi-Heng Jain, Tarun Kumar Aguilar, Carlos A. Karnik, Rohit |
| author2 | Lincoln Laboratory |
| author_facet | Lincoln Laboratory Sen, Yi-Heng Jain, Tarun Kumar Aguilar, Carlos A. Karnik, Rohit |
| author_sort | Sen, Yi-Heng |
| collection | MIT |
| description | Author Manuscript 2013 March 21. |
| first_indexed | 2024-09-23T11:24:42Z |
| format | Article |
| id | mit-1721.1/78877 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:24:42Z |
| publishDate | 2013 |
| publisher | Royal Society of Chemistry, The |
| record_format | dspace |
| spelling | mit-1721.1/788772022-09-27T19:20:22Z Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements Sen, Yi-Heng Jain, Tarun Kumar Aguilar, Carlos A. Karnik, Rohit Lincoln Laboratory Massachusetts Institute of Technology. Department of Mechanical Engineering Sen, Yi-Heng Jain, Tarun Kumar Aguilar, Carlos A. Karnik, Rohit Author Manuscript 2013 March 21. Nanofluidic sensing elements have been the focus of recent experiments for numerous applications ranging from nucleic acid fragment sizing to single-molecule DNA sequencing. These applications critically rely on high measurement fidelity, and methods to increase resolution are required. Herein, we describe fabrication and testing of a nanochannel device that enhances measurement resolution by performing multiple measurements (>100) on single DNA molecules. The enhanced measurement resolution enabled length discrimination between a mixture of λ-DNA (48.5 kbp) and T7 DNA (39.9 kbp) molecules, which were detected as transient current changes during translocation of the molecules through the nanochannel. As long DNA molecules are difficult to resolve quickly and with high fidelity with conventional electrophoresis, this approach may yield potentially portable, direct electrical sizing of DNA fragments with high sensitivity and resolution. National Institutes of Health (U.S.) (Grant R21EB009180) United States. Air Force (Contract FA8721-05-C- 0002) 2013-05-14T14:51:40Z 2013-05-14T14:51:40Z 2012-02 2011-08 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/78877 Sen, Yi-Heng, Tarun Jain, Carlos A. Aguilar, and Rohit Karnik. Enhanced Discrimination of DNA Molecules in Nanofluidic Channels through Multiple Measurements. Lab on a Chip 12(6): 1094, 2012. https://orcid.org/0000-0003-0588-9286 en_US http://dx.doi.org/10.1039/c2lc20771k Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Royal Society of Chemistry, The PubMed Central |
| spellingShingle | Sen, Yi-Heng Jain, Tarun Kumar Aguilar, Carlos A. Karnik, Rohit Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements |
| title | Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements |
| title_full | Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements |
| title_fullStr | Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements |
| title_full_unstemmed | Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements |
| title_short | Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements |
| title_sort | enhanced discrimination of dna molecules in nanofluidic channels through multiple measurements |
| url | http://hdl.handle.net/1721.1/78877 https://orcid.org/0000-0003-0588-9286 |
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