Characterization of Tunable FGF-2 Releasing Polyelectrolyte Multilayers
Fibroblast growth factor 2 (FGF-2) is a potent mediator of stem cell differentiation and proliferation. Although FGF-2 has a well-established role in promoting bone tissue formation, flaws in its delivery have limited its clinical utility. Polyelectrolyte multilayer films represent a novel system fo...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Chemical Society
2013
|
| Online Access: | http://hdl.handle.net/1721.1/79042 https://orcid.org/0000-0003-1727-5732 |
| _version_ | 1826191919983624192 |
|---|---|
| author | Macdonald, Mara L. Rodriguez, Natalia M. Shah, Nisarg J. Hammond, Paula T. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Macdonald, Mara L. Rodriguez, Natalia M. Shah, Nisarg J. Hammond, Paula T. |
| author_sort | Macdonald, Mara L. |
| collection | MIT |
| description | Fibroblast growth factor 2 (FGF-2) is a potent mediator of stem cell differentiation and proliferation. Although FGF-2 has a well-established role in promoting bone tissue formation, flaws in its delivery have limited its clinical utility. Polyelectrolyte multilayer films represent a novel system for FGF-2 delivery that has promise for local, precisely controlled, and sustained release of FGF-2 from surfaces of interest, including medical implants and tissue engineering scaffolds. In this work, the loading and release of FGF-2 from synthetic hydrolytically degradable multilayer thin films of various architectures is explored; drug loading was tunable using at least three parameters (number of nanolayers, counterpolyanion, and type of degradable polycation) and yielded values of 7−45 ng/cm2 of FGF-2. Release time varied between 24 h and approximately five days. FGF-2 released from these films retained in vitro activity, promoting the proliferation of MC3T3 preosteoblast cells. The use of biologically derived counterpolyanions heparin sulfate and chondroitin sulfate in the multilayer structures enhanced FGF-2 activity. The control over drug loading and release kinetics inform future in vivo bone and tissue regeneration models for the exploration of clinical relevance of LbL growth factor delivery films. |
| first_indexed | 2024-09-23T09:03:21Z |
| format | Article |
| id | mit-1721.1/79042 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:03:21Z |
| publishDate | 2013 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | mit-1721.1/790422022-09-30T13:07:08Z Characterization of Tunable FGF-2 Releasing Polyelectrolyte Multilayers Macdonald, Mara L. Rodriguez, Natalia M. Shah, Nisarg J. Hammond, Paula T. Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Chemical Engineering Macdonald, Mara L. Rodriguez, Natalia M. Shah, Nisarg Jaydeep Hammond, Paula T. Fibroblast growth factor 2 (FGF-2) is a potent mediator of stem cell differentiation and proliferation. Although FGF-2 has a well-established role in promoting bone tissue formation, flaws in its delivery have limited its clinical utility. Polyelectrolyte multilayer films represent a novel system for FGF-2 delivery that has promise for local, precisely controlled, and sustained release of FGF-2 from surfaces of interest, including medical implants and tissue engineering scaffolds. In this work, the loading and release of FGF-2 from synthetic hydrolytically degradable multilayer thin films of various architectures is explored; drug loading was tunable using at least three parameters (number of nanolayers, counterpolyanion, and type of degradable polycation) and yielded values of 7−45 ng/cm2 of FGF-2. Release time varied between 24 h and approximately five days. FGF-2 released from these films retained in vitro activity, promoting the proliferation of MC3T3 preosteoblast cells. The use of biologically derived counterpolyanions heparin sulfate and chondroitin sulfate in the multilayer structures enhanced FGF-2 activity. The control over drug loading and release kinetics inform future in vivo bone and tissue regeneration models for the exploration of clinical relevance of LbL growth factor delivery films. Massachusetts Institute of Technology. Deshpande Center for Technological Innovation (Grant 009216-1) National Institutes of Health (U.S.) (Grant 1-R01-AG029601-01) National Science Foundation (U.S.) (Graduate Research Fellowship) 2013-05-30T17:56:13Z 2013-05-30T17:56:13Z 2010-07 2010-06 Article http://purl.org/eprint/type/JournalArticle 1525-7797 1526-4602 http://hdl.handle.net/1721.1/79042 Macdonald, Mara L., Natalia M. Rodriguez, Nisarg J. Shah, and Paula T. Hammond. Characterization of Tunable FGF-2 Releasing Polyelectrolyte Multilayers. Biomacromolecules 11, no. 8 (August 9, 2010): 2053-2059. https://orcid.org/0000-0003-1727-5732 en_US http://dx.doi.org/10.1021/bm100413w Biomacromolecules Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society PMC |
| spellingShingle | Macdonald, Mara L. Rodriguez, Natalia M. Shah, Nisarg J. Hammond, Paula T. Characterization of Tunable FGF-2 Releasing Polyelectrolyte Multilayers |
| title | Characterization of Tunable FGF-2 Releasing Polyelectrolyte Multilayers |
| title_full | Characterization of Tunable FGF-2 Releasing Polyelectrolyte Multilayers |
| title_fullStr | Characterization of Tunable FGF-2 Releasing Polyelectrolyte Multilayers |
| title_full_unstemmed | Characterization of Tunable FGF-2 Releasing Polyelectrolyte Multilayers |
| title_short | Characterization of Tunable FGF-2 Releasing Polyelectrolyte Multilayers |
| title_sort | characterization of tunable fgf 2 releasing polyelectrolyte multilayers |
| url | http://hdl.handle.net/1721.1/79042 https://orcid.org/0000-0003-1727-5732 |
| work_keys_str_mv | AT macdonaldmaral characterizationoftunablefgf2releasingpolyelectrolytemultilayers AT rodrigueznataliam characterizationoftunablefgf2releasingpolyelectrolytemultilayers AT shahnisargj characterizationoftunablefgf2releasingpolyelectrolytemultilayers AT hammondpaulat characterizationoftunablefgf2releasingpolyelectrolytemultilayers |