Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis
Inertial microfluidics has demonstrated the potential to provide a rich range of capabilities to manipulate biological fluids and particles to address various challenges in biomedical science and clinical medicine. Various microchannel geometries have been used to study the inertial focusing behavio...
| Päätekijät: | , , , , |
|---|---|
| Muut tekijät: | |
| Aineistotyyppi: | Artikkeli |
| Kieli: | en_US |
| Julkaistu: |
Royal Society of Chemistry, The
2013
|
| Linkit: | http://hdl.handle.net/1721.1/79094 https://orcid.org/0000-0001-8323-2779 https://orcid.org/0000-0001-6070-7356 |
| _version_ | 1826213239184162816 |
|---|---|
| author | Lim, Eugene J. Edd, Jon F. McKinley, Gareth H. Toner, Mehmet Ober, Thomas Joseph |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Lim, Eugene J. Edd, Jon F. McKinley, Gareth H. Toner, Mehmet Ober, Thomas Joseph |
| author_sort | Lim, Eugene J. |
| collection | MIT |
| description | Inertial microfluidics has demonstrated the potential to provide a rich range of capabilities to manipulate biological fluids and particles to address various challenges in biomedical science and clinical medicine. Various microchannel geometries have been used to study the inertial focusing behavior of particles suspended in simple buffer solutions or in highly diluted blood. One aspect of inertial focusing that has not been studied is how particles suspended in whole or minimally diluted blood respond to inertial forces in microchannels. The utility of imaging techniques (i.e., high-speed bright-field imaging and long exposure fluorescence (streak) imaging) primarily used to observe particle focusing in microchannels is limited in complex fluids such as whole blood due to interference from the large numbers of red blood cells (RBCs). In this study, we used particle trajectory analysis (PTA) to observe the inertial focusing behavior of polystyrene beads, white blood cells, and PC-3 prostate cancer cells in physiological saline and blood. Identification of in-focus (fluorescently labeled) particles was achieved at mean particle velocities of up to 1.85 m s[superscript −1]. Quantitative measurements of in-focus particles were used to construct intensity maps of particle frequency in the channel cross-section and scatter plots of particle centroid coordinates vs. particle diameter. PC-3 cells spiked into whole blood (HCT = 45%) demonstrated a novel focusing mode not observed in physiological saline or diluted blood. PTA can be used as an experimental frame of reference for understanding the physical basis of inertial lift forces in whole blood and discover inertial focusing modes that can be used to enable particle separation in whole blood. |
| first_indexed | 2024-09-23T15:45:39Z |
| format | Article |
| id | mit-1721.1/79094 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:45:39Z |
| publishDate | 2013 |
| publisher | Royal Society of Chemistry, The |
| record_format | dspace |
| spelling | mit-1721.1/790942022-10-02T03:54:07Z Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis Lim, Eugene J. Edd, Jon F. McKinley, Gareth H. Toner, Mehmet Ober, Thomas Joseph Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. School of Engineering Lim, Eugene J. Ober, Thomas Joseph McKinley, Gareth H. Inertial microfluidics has demonstrated the potential to provide a rich range of capabilities to manipulate biological fluids and particles to address various challenges in biomedical science and clinical medicine. Various microchannel geometries have been used to study the inertial focusing behavior of particles suspended in simple buffer solutions or in highly diluted blood. One aspect of inertial focusing that has not been studied is how particles suspended in whole or minimally diluted blood respond to inertial forces in microchannels. The utility of imaging techniques (i.e., high-speed bright-field imaging and long exposure fluorescence (streak) imaging) primarily used to observe particle focusing in microchannels is limited in complex fluids such as whole blood due to interference from the large numbers of red blood cells (RBCs). In this study, we used particle trajectory analysis (PTA) to observe the inertial focusing behavior of polystyrene beads, white blood cells, and PC-3 prostate cancer cells in physiological saline and blood. Identification of in-focus (fluorescently labeled) particles was achieved at mean particle velocities of up to 1.85 m s[superscript −1]. Quantitative measurements of in-focus particles were used to construct intensity maps of particle frequency in the channel cross-section and scatter plots of particle centroid coordinates vs. particle diameter. PC-3 cells spiked into whole blood (HCT = 45%) demonstrated a novel focusing mode not observed in physiological saline or diluted blood. PTA can be used as an experimental frame of reference for understanding the physical basis of inertial lift forces in whole blood and discover inertial focusing modes that can be used to enable particle separation in whole blood. 2013-06-11T19:53:57Z 2013-06-11T19:53:57Z 2012-03 2011-11 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/79094 Lim, Eugene J. et al. “Visualization of Microscale Particle Focusing in Diluted and Whole Blood Using Particle Trajectory Analysis.” Lab on a Chip 12.12 (2012): 2199. https://orcid.org/0000-0001-8323-2779 https://orcid.org/0000-0001-6070-7356 en_US http://dx.doi.org/10.1039/c2lc21100a Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Royal Society of Chemistry, The MIT web domain |
| spellingShingle | Lim, Eugene J. Edd, Jon F. McKinley, Gareth H. Toner, Mehmet Ober, Thomas Joseph Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis |
| title | Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis |
| title_full | Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis |
| title_fullStr | Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis |
| title_full_unstemmed | Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis |
| title_short | Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis |
| title_sort | visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis |
| url | http://hdl.handle.net/1721.1/79094 https://orcid.org/0000-0001-8323-2779 https://orcid.org/0000-0001-6070-7356 |
| work_keys_str_mv | AT limeugenej visualizationofmicroscaleparticlefocusingindilutedandwholebloodusingparticletrajectoryanalysis AT eddjonf visualizationofmicroscaleparticlefocusingindilutedandwholebloodusingparticletrajectoryanalysis AT mckinleygarethh visualizationofmicroscaleparticlefocusingindilutedandwholebloodusingparticletrajectoryanalysis AT tonermehmet visualizationofmicroscaleparticlefocusingindilutedandwholebloodusingparticletrajectoryanalysis AT oberthomasjoseph visualizationofmicroscaleparticlefocusingindilutedandwholebloodusingparticletrajectoryanalysis |