Responsive Microgrooves for the Formation of Harvestable Tissue Constructs
Given its biocompatibility, elasticity, and gas permeability, poly(dimethylsiloxane) (PDMS) is widely used to fabricate microgrooves and microfluidic devices for three-dimensional (3D) cell culture studies. However, conformal coating of complex PDMS devices prepared by standard microfabrication tech...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society
2013
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| Online Access: | http://hdl.handle.net/1721.1/79106 https://orcid.org/0000-0001-6127-1056 https://orcid.org/0000-0003-4255-0492 |
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| author | Tekin, Halil Ozaydin-Ince, Gozde Tsinman, Tonia Khademhosseini, Ali Demirel, Melik C. Gleason, Karen K Langer, Robert S |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Tekin, Halil Ozaydin-Ince, Gozde Tsinman, Tonia Khademhosseini, Ali Demirel, Melik C. Gleason, Karen K Langer, Robert S |
| author_sort | Tekin, Halil |
| collection | MIT |
| description | Given its biocompatibility, elasticity, and gas permeability, poly(dimethylsiloxane) (PDMS) is widely used to fabricate microgrooves and microfluidic devices for three-dimensional (3D) cell culture studies. However, conformal coating of complex PDMS devices prepared by standard microfabrication techniques with desired chemical functionality is challenging. This study describes the conformal coating of PDMS microgrooves with poly(N-isopropylacrylamide) (PNIPAAm) by using initiated chemical vapor deposition (iCVD). These microgrooves guided the formation of tissue constructs from NIH-3T3 fibroblasts that could be retrieved by the temperature-dependent swelling property and hydrophilicity change of the PNIPAAm. The thickness of swollen PNIPAAm films at 24 °C was approximately 3 times greater than at 37 °C. Furthermore, PNIPAAm-coated microgroove surfaces exhibit increased hydrophilicity at 24 °C (contact angle θ = 30° ± 2) compared to 37 °C (θ = 50° ± 1). Thus PNIPAAm film on the microgrooves exhibits responsive swelling with higher hydrophilicity at room temperature, which could be used to retrieve tissue constructs. The resulting tissue constructs were the same size as the grooves and could be used as modules in tissue fabrication. Given its ability to form and retrieve cell aggregates and its integration with standard microfabrication, PNIPAAm-coated PDMS templates may become useful for 3D cell culture applications in tissue engineering and drug discovery. |
| first_indexed | 2024-09-23T09:50:30Z |
| format | Article |
| id | mit-1721.1/79106 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:50:30Z |
| publishDate | 2013 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | mit-1721.1/791062022-09-26T14:02:33Z Responsive Microgrooves for the Formation of Harvestable Tissue Constructs Tekin, Halil Ozaydin-Ince, Gozde Tsinman, Tonia Khademhosseini, Ali Demirel, Melik C. Gleason, Karen K Langer, Robert S Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Tekin, Halil Ozaydin-Ince, Gozde Gleason, Karen K. Langer, Robert Khademhosseini, Ali Demirel, Melik C. Given its biocompatibility, elasticity, and gas permeability, poly(dimethylsiloxane) (PDMS) is widely used to fabricate microgrooves and microfluidic devices for three-dimensional (3D) cell culture studies. However, conformal coating of complex PDMS devices prepared by standard microfabrication techniques with desired chemical functionality is challenging. This study describes the conformal coating of PDMS microgrooves with poly(N-isopropylacrylamide) (PNIPAAm) by using initiated chemical vapor deposition (iCVD). These microgrooves guided the formation of tissue constructs from NIH-3T3 fibroblasts that could be retrieved by the temperature-dependent swelling property and hydrophilicity change of the PNIPAAm. The thickness of swollen PNIPAAm films at 24 °C was approximately 3 times greater than at 37 °C. Furthermore, PNIPAAm-coated microgroove surfaces exhibit increased hydrophilicity at 24 °C (contact angle θ = 30° ± 2) compared to 37 °C (θ = 50° ± 1). Thus PNIPAAm film on the microgrooves exhibits responsive swelling with higher hydrophilicity at room temperature, which could be used to retrieve tissue constructs. The resulting tissue constructs were the same size as the grooves and could be used as modules in tissue fabrication. Given its ability to form and retrieve cell aggregates and its integration with standard microfabrication, PNIPAAm-coated PDMS templates may become useful for 3D cell culture applications in tissue engineering and drug discovery. Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies (project DAAD-19-02-D-002) Charles Stark Draper Laboratory National Institutes of Health (U.S.) (DE01323) National Institutes of Health (U.S.) (DE016516) National Institutes of Health (U.S.) (HL092836) National Institutes of Health (U.S.) (DE019024) National Institutes of Health (U.S.) (EB007249) National Science Foundation (U.S.) (NSF Career Award (DMR0- 847287)) United States. Office of Naval Research (Young Investigator Award) Wyss Institute for Biologically Inspired Engineering 2013-06-14T14:41:19Z 2013-06-14T14:41:19Z 2011-03 2011-03 Article http://purl.org/eprint/type/JournalArticle 0743-7463 1520-5827 http://hdl.handle.net/1721.1/79106 Tekin, Halil, Gozde Ozaydin-Ince, Tonia Tsinman, Karen K. Gleason, Robert Langer, Ali Khademhosseini, and Melik C. Demirel. Responsive Microgrooves for the Formation of Harvestable Tissue Constructs. Langmuir 27, no. 9: 5671-5679. https://orcid.org/0000-0001-6127-1056 https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1021/la200183x Langmuir Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society PMC |
| spellingShingle | Tekin, Halil Ozaydin-Ince, Gozde Tsinman, Tonia Khademhosseini, Ali Demirel, Melik C. Gleason, Karen K Langer, Robert S Responsive Microgrooves for the Formation of Harvestable Tissue Constructs |
| title | Responsive Microgrooves for the Formation of Harvestable Tissue Constructs |
| title_full | Responsive Microgrooves for the Formation of Harvestable Tissue Constructs |
| title_fullStr | Responsive Microgrooves for the Formation of Harvestable Tissue Constructs |
| title_full_unstemmed | Responsive Microgrooves for the Formation of Harvestable Tissue Constructs |
| title_short | Responsive Microgrooves for the Formation of Harvestable Tissue Constructs |
| title_sort | responsive microgrooves for the formation of harvestable tissue constructs |
| url | http://hdl.handle.net/1721.1/79106 https://orcid.org/0000-0001-6127-1056 https://orcid.org/0000-0003-4255-0492 |
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