Ultra-rapid 2-D and 3-D laser microprinting of proteins
Thesis (Ph. D. in Electrical and Medical Engineering)--Harvard-MIT Program in Health Sciences and Technology, 2013.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2013
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| Online Access: | http://hdl.handle.net/1721.1/79248 |
| _version_ | 1811083154986893312 |
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| author | Scott, Mark Andrew, Ph. D. Massachusetts Institute of Technology |
| author2 | Mehmet Faith Yanik. |
| author_facet | Mehmet Faith Yanik. Scott, Mark Andrew, Ph. D. Massachusetts Institute of Technology |
| author_sort | Scott, Mark Andrew, Ph. D. Massachusetts Institute of Technology |
| collection | MIT |
| description | Thesis (Ph. D. in Electrical and Medical Engineering)--Harvard-MIT Program in Health Sciences and Technology, 2013. |
| first_indexed | 2024-09-23T12:25:26Z |
| format | Thesis |
| id | mit-1721.1/79248 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T12:25:26Z |
| publishDate | 2013 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/792482019-04-10T18:46:26Z Ultra-rapid 2-D and 3-D laser microprinting of proteins Ultra-rapid two-D and three-D laser microprinting of proteins Ultra-rapid two-dimensional and three-dimensional laser microprinting of proteins Scott, Mark Andrew, Ph. D. Massachusetts Institute of Technology Mehmet Faith Yanik. Harvard--MIT Program in Health Sciences and Technology. Harvard--MIT Program in Health Sciences and Technology. Harvard--MIT Program in Health Sciences and Technology. Thesis (Ph. D. in Electrical and Medical Engineering)--Harvard-MIT Program in Health Sciences and Technology, 2013. Cataloged from PDF version of thesis. Includes bibliographical references (p. 124-135). When viewed under the microscope, biological tissues reveal an exquisite microarchitecture. These complex patterns arise during development, as cells interact with a multitude of chemical and mechanical cues in the surrounding extracellular matrix. Tissue engineers have sought for decades to repair or replace damaged tissue, often relying on porous scaffolds as an artificial extracellular matrix to support cell development. However, these grafts are unable to recapitulate the complexity of the in vivo environment, limiting our ability to regenerate functional tissue. Biomedical engineers have developed several methods for printing two- and three-dimensional patterns of proteins for studying and directing cell development. Of these methods, laser microprinting of proteins has shown the most promise for printing sub-cellular resolution gradients of cues, but the photochemistry remains too slow to enable large-scale applications for screening and therapeutics In this work, we demonstrate a novel high-speed photochemistry based on multi-photon photobleaching of fluorescein, and we build the fastest 2-D and 3-D laser microprinter for proteins to date. First, we show that multiphoton photobleaching of a deoxygenated solution of biotin-4-fluorescein onto a PEG monolayer with acrylate end-group can enable print speeds of almost 20 million pixels per second at 600 nanometer resolution. We discovered that the mechanism of fluorescein photobleaching evolves from a 2-photon to 3- and 4-photon regime at higher laser intensities, unlocking faster printing kinetics. Using this 2-D printing system, we develop a novel triangle-ratchet method for directing the polarization of single hippocampal neurons. This ability to determine which neurite becomes an axon, and which neuritis become dendrites is an essential step for developing defined in vitro neural networks. Next, we modify our multiphoton photobleaching system to print in three dimensions. For the first time, we demonstrate 3-D printing of full length proteins in collagen, fibrin and gelatin methacrylate scaffolds, as well as printing in agarose and agarose methacrylate scaffolds. We also present a novel method for 3-D printing collagen scaffolds at unprecedented speeds, up to 14 layers per second, generating complex shapes in seconds with sub-micron resolution. Finally, we demonstrate that 3-D printing of scaffold architecture and protein cues inside the scaffold can be combined, for the first time enabling structures with complex sub-micron architectures and chemical cues for directing development. We believe that the ultra-rapid printing technology presented in this thesis will be a key enabler in the development of complex, artificially engineered tissues and organs. by Mark Andrew Scott. Ph.D.in Electrical and Medical Engineering 2013-06-17T19:50:28Z 2013-06-17T19:50:28Z 2013 2013 Thesis http://hdl.handle.net/1721.1/79248 846475273 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 135 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Harvard--MIT Program in Health Sciences and Technology. Scott, Mark Andrew, Ph. D. Massachusetts Institute of Technology Ultra-rapid 2-D and 3-D laser microprinting of proteins |
| title | Ultra-rapid 2-D and 3-D laser microprinting of proteins |
| title_full | Ultra-rapid 2-D and 3-D laser microprinting of proteins |
| title_fullStr | Ultra-rapid 2-D and 3-D laser microprinting of proteins |
| title_full_unstemmed | Ultra-rapid 2-D and 3-D laser microprinting of proteins |
| title_short | Ultra-rapid 2-D and 3-D laser microprinting of proteins |
| title_sort | ultra rapid 2 d and 3 d laser microprinting of proteins |
| topic | Harvard--MIT Program in Health Sciences and Technology. |
| url | http://hdl.handle.net/1721.1/79248 |
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