Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes
An emerging concept in cell signalling is the natural role of reactive oxygen species such as hydrogen peroxide (H₂O₂) as beneficial messengers in redox signalling pathways. The nature of H₂O₂ signalling is confounded, however, by difficulties in tracking it in living systems, both spatially and tem...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2013
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| Online Access: | http://hdl.handle.net/1721.1/79353 https://orcid.org/0000-0003-2944-808X |
| _version_ | 1811088761294946304 |
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| author | Jin, Hong Heller, Daniel A. Kalbacova, Marie Kim, Jong-Ho Zhang, Jingqing Boghossian, Ardemis A. Maheshri, Narendra Strano, Michael S. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Jin, Hong Heller, Daniel A. Kalbacova, Marie Kim, Jong-Ho Zhang, Jingqing Boghossian, Ardemis A. Maheshri, Narendra Strano, Michael S. |
| author_sort | Jin, Hong |
| collection | MIT |
| description | An emerging concept in cell signalling is the natural role of reactive oxygen species such as hydrogen peroxide (H₂O₂) as beneficial messengers in redox signalling pathways. The nature of H₂O₂ signalling is confounded, however, by difficulties in tracking it in living systems, both spatially and temporally, at low concentrations. Here, we develop an array of fluorescent single-walled carbon nanotubes that can selectively record, in real time, the discrete, stochastic quenching events that occur as H₂O₂molecules are emitted from individual human epidermal carcinoma cells stimulated by epidermal growth factor. We show mathematically that such arrays can distinguish between molecules originating locally on the cell membrane from other contributions. We find that epidermal growth factor induces 2 nmol H₂O₂ locally over a period of 50 min. This platform promises a new approach to understanding the signalling of reactive oxygen species at the cellular level. |
| first_indexed | 2024-09-23T14:07:06Z |
| format | Article |
| id | mit-1721.1/79353 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:07:06Z |
| publishDate | 2013 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/793532022-09-28T18:36:25Z Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes Jin, Hong Heller, Daniel A. Kalbacova, Marie Kim, Jong-Ho Zhang, Jingqing Boghossian, Ardemis A. Maheshri, Narendra Strano, Michael S. Massachusetts Institute of Technology. Department of Chemical Engineering Jin, Hong Heller, Daniel A. Kim, Jong-Ho Zhang, Jingqing Boghossian, Ardemis A. Maheshri, Narendra Strano, Michael S. An emerging concept in cell signalling is the natural role of reactive oxygen species such as hydrogen peroxide (H₂O₂) as beneficial messengers in redox signalling pathways. The nature of H₂O₂ signalling is confounded, however, by difficulties in tracking it in living systems, both spatially and temporally, at low concentrations. Here, we develop an array of fluorescent single-walled carbon nanotubes that can selectively record, in real time, the discrete, stochastic quenching events that occur as H₂O₂molecules are emitted from individual human epidermal carcinoma cells stimulated by epidermal growth factor. We show mathematically that such arrays can distinguish between molecules originating locally on the cell membrane from other contributions. We find that epidermal growth factor induces 2 nmol H₂O₂ locally over a period of 50 min. This platform promises a new approach to understanding the signalling of reactive oxygen species at the cellular level. Arnold and Mabel Beckman Foundation (Beckman Young Investigator award) National Science Foundation (U.S.) ((NSF) Career Award) National Science Foundation (U.S.) (Nanoscale Interdisciplinary Research Team) Czech Republic. Ministry of Education, Youth, and Sports (project no. MSM0021620806) Czech Republic. Ministry of Education, Youth, and Sports (KAN grant no. 400100701) 2013-06-20T16:06:28Z 2013-06-20T16:06:28Z 2010-03 2009-09 Article http://purl.org/eprint/type/JournalArticle 1748-3387 1748-3395 http://hdl.handle.net/1721.1/79353 Jin, Hong, Daniel A. Heller, Marie Kalbacova, Jong-Ho Kim, Jingqing Zhang, Ardemis A. Boghossian, Narendra Maheshri, and Michael S. Strano. Detection of Single-molecule H₂O₂ Signalling from Epidermal Growth Factor Receptor Using Fluorescent Single-walled Carbon Nanotubes. Nature Nanotechnology 5, no. 4 (March 7, 2010): 302-309. https://orcid.org/0000-0003-2944-808X en_US http://dx.doi.org/10.1038/nnano.2010.24 Nature Nanotechnology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group MIT Web Domain |
| spellingShingle | Jin, Hong Heller, Daniel A. Kalbacova, Marie Kim, Jong-Ho Zhang, Jingqing Boghossian, Ardemis A. Maheshri, Narendra Strano, Michael S. Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes |
| title | Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes |
| title_full | Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes |
| title_fullStr | Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes |
| title_full_unstemmed | Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes |
| title_short | Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes |
| title_sort | detection of single molecule h₂o₂ signalling from epidermal growth factor receptor using fluorescent single walled carbon nanotubes |
| url | http://hdl.handle.net/1721.1/79353 https://orcid.org/0000-0003-2944-808X |
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