Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates
We demonstrate rapid microfluidic fabrication of hybrid microparticles composed of functionalized viral nanotemplates directly embedded in polymeric hydrogels. Specifically, genetically modified tobacco mosaic virus (TMV) templates were covalently labeled with fluorescent markers or metalized with p...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society
2013
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| Online Access: | http://hdl.handle.net/1721.1/79365 https://orcid.org/0000-0002-1381-8923 |
| _version_ | 1826194890165321728 |
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| author | Lewis, Christina L. Lin, Yan Yang, Cuixian Manocchi, Amy K. Yuet, Kai P. Doyle, Patrick S. Yi, Hyunmin |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Lewis, Christina L. Lin, Yan Yang, Cuixian Manocchi, Amy K. Yuet, Kai P. Doyle, Patrick S. Yi, Hyunmin |
| author_sort | Lewis, Christina L. |
| collection | MIT |
| description | We demonstrate rapid microfluidic fabrication of hybrid microparticles composed of functionalized viral nanotemplates directly embedded in polymeric hydrogels. Specifically, genetically modified tobacco mosaic virus (TMV) templates were covalently labeled with fluorescent markers or metalized with palladium (Pd) nanoparticles (Pd-TMV) and then suspended in a poly(ethylene glycol)-based solution. Upon formation in a flow-focusing device, droplets were photopolymerized with UV light to form microparticles. Fluorescence and confocal microscopy images of microparticles containing fluorescently labeled TMV show uniform distribution of TMV nanotemplates throughout the microparticles. Catalytic activity, via the dichromate reduction reaction, is also demonstrated with microparticles containing Pd−TMV complexes. Additionally, Janus microparticles were fabricated containing viruses embedded in one side and magnetic nanoparticles in the other, which enabled simple separation from bulk solution. These results represent a facile route to directly harness the advantages of viral nanotemplates into a readily usable and stable 3D assembled format. |
| first_indexed | 2024-09-23T10:03:27Z |
| format | Article |
| id | mit-1721.1/79365 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:03:27Z |
| publishDate | 2013 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | mit-1721.1/793652022-09-30T18:37:06Z Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates Lewis, Christina L. Lin, Yan Yang, Cuixian Manocchi, Amy K. Yuet, Kai P. Doyle, Patrick S. Yi, Hyunmin Massachusetts Institute of Technology. Department of Chemical Engineering Doyle, Patrick S. Yuet, Kai P. We demonstrate rapid microfluidic fabrication of hybrid microparticles composed of functionalized viral nanotemplates directly embedded in polymeric hydrogels. Specifically, genetically modified tobacco mosaic virus (TMV) templates were covalently labeled with fluorescent markers or metalized with palladium (Pd) nanoparticles (Pd-TMV) and then suspended in a poly(ethylene glycol)-based solution. Upon formation in a flow-focusing device, droplets were photopolymerized with UV light to form microparticles. Fluorescence and confocal microscopy images of microparticles containing fluorescently labeled TMV show uniform distribution of TMV nanotemplates throughout the microparticles. Catalytic activity, via the dichromate reduction reaction, is also demonstrated with microparticles containing Pd−TMV complexes. Additionally, Janus microparticles were fabricated containing viruses embedded in one side and magnetic nanoparticles in the other, which enabled simple separation from bulk solution. These results represent a facile route to directly harness the advantages of viral nanotemplates into a readily usable and stable 3D assembled format. National Science Foundation (U.S.) (Grant CBET-0941538) National Science Foundation (U.S.) (Grant DMR-1006613) National Science Foundation (U.S.) (Grant DMR- 1006147) National Institute of General Medical Sciences (U.S.) (Award K12GM074869 (TEACRS)) 2013-06-21T16:10:49Z 2013-06-21T16:10:49Z 2010-08 2010-07 Article http://purl.org/eprint/type/JournalArticle 0743-7463 1520-5827 http://hdl.handle.net/1721.1/79365 Lewis, Christina L., Yan Lin, Cuixian Yang, Amy K. Manocchi, Kai P. Yuet, Patrick S. Doyle, and Hyunmin Yi. Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates. Langmuir 26, no. 16 (August 17, 2010): 13436-13441. https://orcid.org/0000-0002-1381-8923 en_US http://dx.doi.org/10.1021/la102446n Langmuir Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society PMC |
| spellingShingle | Lewis, Christina L. Lin, Yan Yang, Cuixian Manocchi, Amy K. Yuet, Kai P. Doyle, Patrick S. Yi, Hyunmin Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates |
| title | Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates |
| title_full | Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates |
| title_fullStr | Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates |
| title_full_unstemmed | Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates |
| title_short | Microfluidic Fabrication of Hydrogel Microparticles Containing Functionalized Viral Nanotemplates |
| title_sort | microfluidic fabrication of hydrogel microparticles containing functionalized viral nanotemplates |
| url | http://hdl.handle.net/1721.1/79365 https://orcid.org/0000-0002-1381-8923 |
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