Single-Cell Analysis Reveals Isotype-Specific Autoreactive B Cell Repertoires in Sjögren’s Syndrome
Microengraving is a novel technology that uses an array of microfabricated subnanoliter wells to isolate and characterize secreted proteins from larger number of single cells. This printing technique permits the capture and characterization of secreted antibodies on glass slides. Here, we profiled t...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Public Library of Science
2013
|
| Online Access: | http://hdl.handle.net/1721.1/79395 https://orcid.org/0000-0003-0921-3144 |
| _version_ | 1826195379222216704 |
|---|---|
| author | Nguyen, Cuong Q. Ogunniyi, Adebola Oluwakayode Karabiyik, Afife Love, John C |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Nguyen, Cuong Q. Ogunniyi, Adebola Oluwakayode Karabiyik, Afife Love, John C |
| author_sort | Nguyen, Cuong Q. |
| collection | MIT |
| description | Microengraving is a novel technology that uses an array of microfabricated subnanoliter wells to isolate and characterize secreted proteins from larger number of single cells. This printing technique permits the capture and characterization of secreted antibodies on glass slides. Here, we profiled the antigenic repertoires of B cells reacting against salivary gland tissues in Sjögren’s syndrome (SjS), an autoimmune disease targeting the exocrine glands. Single-cell suspensions of spleen and cervical lymph node cells prepared from normal C57BL/6 and SjS-susceptible (SjSs) C57BL/6.NOD-AecAec2 mice were dispersed into subnanoliter wells (nanowells). Capture slides preincubated with mouse immunoglobulins were used for printing. Detection antibodies included fluorescence conjugated anti-IgG1, salivary gland lysates of C57BL/6 and SjSs mice. Results indicate an increase in the frequency of IgG1-secreting cells in the spleen of SjSs mice compared to C57BL/6 mice. Cells from the lymph node of SjSs mice yield higher instances of IgG1 reactive against salivary gland antigens than cells from the lymph nodes of C57BL/6 mice. These data demonstrate the isotype-specific reactivity of antibodies during the autoimmune process, and further reveals significant differences in the non-autoimmune and autoimmune antibody repertoires. These results support the generation of self-reactive B cell repertoires during the autoimmune process, at the same time, verifying that microengraving of single cells might allow for identification of novel biomarkers in SjS. |
| first_indexed | 2024-09-23T10:11:44Z |
| format | Article |
| id | mit-1721.1/79395 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:11:44Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/793952022-09-30T19:30:39Z Single-Cell Analysis Reveals Isotype-Specific Autoreactive B Cell Repertoires in Sjögren’s Syndrome Nguyen, Cuong Q. Ogunniyi, Adebola Oluwakayode Karabiyik, Afife Love, John C Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Love, J. Christopher Ogunniyi, Adebola Oluwakayode Microengraving is a novel technology that uses an array of microfabricated subnanoliter wells to isolate and characterize secreted proteins from larger number of single cells. This printing technique permits the capture and characterization of secreted antibodies on glass slides. Here, we profiled the antigenic repertoires of B cells reacting against salivary gland tissues in Sjögren’s syndrome (SjS), an autoimmune disease targeting the exocrine glands. Single-cell suspensions of spleen and cervical lymph node cells prepared from normal C57BL/6 and SjS-susceptible (SjSs) C57BL/6.NOD-AecAec2 mice were dispersed into subnanoliter wells (nanowells). Capture slides preincubated with mouse immunoglobulins were used for printing. Detection antibodies included fluorescence conjugated anti-IgG1, salivary gland lysates of C57BL/6 and SjSs mice. Results indicate an increase in the frequency of IgG1-secreting cells in the spleen of SjSs mice compared to C57BL/6 mice. Cells from the lymph node of SjSs mice yield higher instances of IgG1 reactive against salivary gland antigens than cells from the lymph nodes of C57BL/6 mice. These data demonstrate the isotype-specific reactivity of antibodies during the autoimmune process, and further reveals significant differences in the non-autoimmune and autoimmune antibody repertoires. These results support the generation of self-reactive B cell repertoires during the autoimmune process, at the same time, verifying that microengraving of single cells might allow for identification of novel biomarkers in SjS. Sjogren's Syndrome Foundation National Institute of Dental and Craniofacial Research (U.S.) (PHS Grant R00 DE018958) National Institutes of Health (U.S.) (NIH/NIAID (RC1AI086152)) 2013-07-01T17:15:21Z 2013-07-01T17:15:21Z 2013-03 2012-10 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/79395 Nguyen, Cuong Q., Adebola O. Ogunniyi, Afife Karabiyik, and J. Christopher Love. Single-Cell Analysis Reveals Isotype-Specific Autoreactive B Cell Repertoires in Sjögren’s Syndrome. Edited by John A. Chiorini. PLoS ONE 8, no. 3 (March 13, 2013): e58127. https://orcid.org/0000-0003-0921-3144 en_US http://dx.doi.org/10.1371/journal.pone.0058127 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Nguyen, Cuong Q. Ogunniyi, Adebola Oluwakayode Karabiyik, Afife Love, John C Single-Cell Analysis Reveals Isotype-Specific Autoreactive B Cell Repertoires in Sjögren’s Syndrome |
| title | Single-Cell Analysis Reveals Isotype-Specific Autoreactive B Cell Repertoires in Sjögren’s Syndrome |
| title_full | Single-Cell Analysis Reveals Isotype-Specific Autoreactive B Cell Repertoires in Sjögren’s Syndrome |
| title_fullStr | Single-Cell Analysis Reveals Isotype-Specific Autoreactive B Cell Repertoires in Sjögren’s Syndrome |
| title_full_unstemmed | Single-Cell Analysis Reveals Isotype-Specific Autoreactive B Cell Repertoires in Sjögren’s Syndrome |
| title_short | Single-Cell Analysis Reveals Isotype-Specific Autoreactive B Cell Repertoires in Sjögren’s Syndrome |
| title_sort | single cell analysis reveals isotype specific autoreactive b cell repertoires in sjogren s syndrome |
| url | http://hdl.handle.net/1721.1/79395 https://orcid.org/0000-0003-0921-3144 |
| work_keys_str_mv | AT nguyencuongq singlecellanalysisrevealsisotypespecificautoreactivebcellrepertoiresinsjogrenssyndrome AT ogunniyiadebolaoluwakayode singlecellanalysisrevealsisotypespecificautoreactivebcellrepertoiresinsjogrenssyndrome AT karabiyikafife singlecellanalysisrevealsisotypespecificautoreactivebcellrepertoiresinsjogrenssyndrome AT lovejohnc singlecellanalysisrevealsisotypespecificautoreactivebcellrepertoiresinsjogrenssyndrome |