Development of multiplexing strategies for electron and super-resolution optical microscopy/
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2013.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2013
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| Online Access: | http://hdl.handle.net/1721.1/79544 |
| _version_ | 1826217052618096640 |
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| author | Tillberg, Paul W |
| author2 | Edward S. Boyden. |
| author_facet | Edward S. Boyden. Tillberg, Paul W |
| author_sort | Tillberg, Paul W |
| collection | MIT |
| description | Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2013. |
| first_indexed | 2024-09-23T16:57:17Z |
| format | Thesis |
| id | mit-1721.1/79544 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T16:57:17Z |
| publishDate | 2013 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/795442019-04-11T11:11:35Z Development of multiplexing strategies for electron and super-resolution optical microscopy/ Tillberg, Paul W Edward S. Boyden. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science. Electrical Engineering and Computer Science. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2013. Cataloged from PDF version of thesis. Includes bibliographical references (p. 30-31). The aim of this work is to increase the multiplexing capabilities of electron and super resolution optical microscopy. This will be done through the development of molecular-scale barcodes that can be resolved in one of the two high resolution imaging modes. In the optical domain, the number of colors available in stochastic optical reconstruction microscopy (STORM) will be increased by taking advantage of not only the spectral differences between STORM fluorophores but their kinetic properties as well. In the electron microscopy domain, the recently developed electron contrast-generating protein miniSOG will be concatenated to produce fully genetically encoded barcodes that can be resolved using standard transmission electron microscopy techniques. At the time of writing, the hardware for a STORM microscope has been assembled. Single molecule fluorescence blinking has been observed, though the imaging buffer still needs to be optimized for imaging. Concatamers of miniSOG have been generated and can be expressed in HEK cells and photo-oxidized. by Paul W. Tillberg. S.M. 2013-07-10T14:53:25Z 2013-07-10T14:53:25Z 2013 2013 Thesis http://hdl.handle.net/1721.1/79544 851388135 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 31 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Electrical Engineering and Computer Science. Tillberg, Paul W Development of multiplexing strategies for electron and super-resolution optical microscopy/ |
| title | Development of multiplexing strategies for electron and super-resolution optical microscopy/ |
| title_full | Development of multiplexing strategies for electron and super-resolution optical microscopy/ |
| title_fullStr | Development of multiplexing strategies for electron and super-resolution optical microscopy/ |
| title_full_unstemmed | Development of multiplexing strategies for electron and super-resolution optical microscopy/ |
| title_short | Development of multiplexing strategies for electron and super-resolution optical microscopy/ |
| title_sort | development of multiplexing strategies for electron and super resolution optical microscopy |
| topic | Electrical Engineering and Computer Science. |
| url | http://hdl.handle.net/1721.1/79544 |
| work_keys_str_mv | AT tillbergpaulw developmentofmultiplexingstrategiesforelectronandsuperresolutionopticalmicroscopy |