Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast
Recent analysis of genomic signatures in mammals, flies, and worms indicates that functional translational stop codon readthrough is considerably more abundant in metazoa than previously recognized, but this analysis provides only limited clues about the function or mechanism of readthrough. If an m...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Public Library of Science
2013
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| Online Access: | http://hdl.handle.net/1721.1/79593 https://orcid.org/0000-0002-7852-4328 |
| _version_ | 1826195787825020928 |
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| author | Jungreis, Irwin Kellis, Manolis Chan, Clara Sophia |
| author2 | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory |
| author_facet | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Jungreis, Irwin Kellis, Manolis Chan, Clara Sophia |
| author_sort | Jungreis, Irwin |
| collection | MIT |
| description | Recent analysis of genomic signatures in mammals, flies, and worms indicates that functional translational stop codon readthrough is considerably more abundant in metazoa than previously recognized, but this analysis provides only limited clues about the function or mechanism of readthrough. If an mRNA known to be read through in one species is also read through in another, perhaps these questions can be studied in a simpler setting. With this end in mind, we have investigated whether some of the readthrough genes in human, fly, and worm also exhibit readthrough when expressed in S. cerevisiae. We found that readthrough was highest in a gene with a post-stop hexamer known to trigger readthrough, while other metazoan readthrough genes exhibit borderline readthrough in S. cerevisiae. |
| first_indexed | 2024-09-23T10:15:26Z |
| format | Article |
| id | mit-1721.1/79593 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:15:26Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/795932022-09-26T16:47:16Z Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast Jungreis, Irwin Kellis, Manolis Chan, Clara Sophia Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Mathematics Chan, Clara Sophia Jungreis, Irwin Kellis, Manolis Recent analysis of genomic signatures in mammals, flies, and worms indicates that functional translational stop codon readthrough is considerably more abundant in metazoa than previously recognized, but this analysis provides only limited clues about the function or mechanism of readthrough. If an mRNA known to be read through in one species is also read through in another, perhaps these questions can be studied in a simpler setting. With this end in mind, we have investigated whether some of the readthrough genes in human, fly, and worm also exhibit readthrough when expressed in S. cerevisiae. We found that readthrough was highest in a gene with a post-stop hexamer known to trigger readthrough, while other metazoan readthrough genes exhibit borderline readthrough in S. cerevisiae. National Institutes of Health (U.S.) (5U54HG004555-03) 2013-07-12T16:54:33Z 2013-07-12T16:54:33Z 2013-03 2013-01 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/79593 Chan, Clara S., Irwin Jungreis, and Manolis Kellis 2013Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast. Joseph Schacherer, ed. PLoS ONE 8(3): e59450. https://orcid.org/0000-0002-7852-4328 en_US http://dx.doi.org/10.1371/journal.pone.0059450 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Jungreis, Irwin Kellis, Manolis Chan, Clara Sophia Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast |
| title | Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast |
| title_full | Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast |
| title_fullStr | Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast |
| title_full_unstemmed | Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast |
| title_short | Heterologous Stop Codon Readthrough of Metazoan Readthrough Candidates in Yeast |
| title_sort | heterologous stop codon readthrough of metazoan readthrough candidates in yeast |
| url | http://hdl.handle.net/1721.1/79593 https://orcid.org/0000-0002-7852-4328 |
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