Automated whole-cell patch-clamp electrophysiology of neurons in vivo
Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorit...
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2013
|
| Online Access: | http://hdl.handle.net/1721.1/79604 https://orcid.org/0000-0003-2218-7489 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0001-7132-8706 |
| _version_ | 1811076436809744384 |
|---|---|
| author | Kodandaramaiah, Suhasa Bangalo Talei Franzesi, Giovanni Forest, Craig R. Chow, Brian Yichiun Boyden, Edward |
| author2 | Massachusetts Institute of Technology. Media Laboratory |
| author_facet | Massachusetts Institute of Technology. Media Laboratory Kodandaramaiah, Suhasa Bangalo Talei Franzesi, Giovanni Forest, Craig R. Chow, Brian Yichiun Boyden, Edward |
| author_sort | Kodandaramaiah, Suhasa Bangalo |
| collection | MIT |
| description | Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and hippocampus. |
| first_indexed | 2024-09-23T10:22:05Z |
| format | Article |
| id | mit-1721.1/79604 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:22:05Z |
| publishDate | 2013 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/796042022-05-26T02:12:57Z Automated whole-cell patch-clamp electrophysiology of neurons in vivo Kodandaramaiah, Suhasa Bangalo Talei Franzesi, Giovanni Forest, Craig R. Chow, Brian Yichiun Boyden, Edward Massachusetts Institute of Technology. Media Laboratory McGovern Institute for Brain Research at MIT Program in Media Arts and Sciences (Massachusetts Institute of Technology) Kodandaramaiah, Suhasa Bangalo Talei Franzesi, Giovanni Chow, Brian Y. Boyden, Edward Stuart Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and hippocampus. National Institutes of Health (U.S.) (NIH EUREKA Award program (1R01NS075421)) National Institutes of Health (U.S.) ((NIH) Director′s New Innovator Award (DP2OD002002) National Science Foundation (U.S.) ((NSF) CAREER award (CBET 1053233)) New York Stem Cell Foundation (Robertson Neuroscience Award) Dr. Gerald Burnett and Marjorie Burnett National Science Foundation (U.S.) (grant CISE 1110947) National Science Foundation (U.S.) (grant EHR 0965945) American Heart Association (10GRNT4430029) 2013-07-16T21:38:18Z 2013-07-16T21:38:18Z 2012-05 Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/79604 Kodandaramaiah, Suhasa B, Giovanni Talei Franzesi, Brian Y Chow, Edward S Boyden, and Craig R Forest. Automated Whole-cell Patch-clamp Electrophysiology of Neurons in Vivo. Nature Methods 9, no. 6 (May 6, 2012): 585-587. https://orcid.org/0000-0003-2218-7489 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0001-7132-8706 en_US http://dx.doi.org/10.1038/nmeth.1993 Nature Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Kodandaramaiah, Suhasa Bangalo Talei Franzesi, Giovanni Forest, Craig R. Chow, Brian Yichiun Boyden, Edward Automated whole-cell patch-clamp electrophysiology of neurons in vivo |
| title | Automated whole-cell patch-clamp electrophysiology of neurons in vivo |
| title_full | Automated whole-cell patch-clamp electrophysiology of neurons in vivo |
| title_fullStr | Automated whole-cell patch-clamp electrophysiology of neurons in vivo |
| title_full_unstemmed | Automated whole-cell patch-clamp electrophysiology of neurons in vivo |
| title_short | Automated whole-cell patch-clamp electrophysiology of neurons in vivo |
| title_sort | automated whole cell patch clamp electrophysiology of neurons in vivo |
| url | http://hdl.handle.net/1721.1/79604 https://orcid.org/0000-0003-2218-7489 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0001-7132-8706 |
| work_keys_str_mv | AT kodandaramaiahsuhasabangalo automatedwholecellpatchclampelectrophysiologyofneuronsinvivo AT taleifranzesigiovanni automatedwholecellpatchclampelectrophysiologyofneuronsinvivo AT forestcraigr automatedwholecellpatchclampelectrophysiologyofneuronsinvivo AT chowbrianyichiun automatedwholecellpatchclampelectrophysiologyofneuronsinvivo AT boydenedward automatedwholecellpatchclampelectrophysiologyofneuronsinvivo |