Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses
available in PMC 2011 September 1
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2013
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| Online Access: | http://hdl.handle.net/1721.1/79763 https://orcid.org/0000-0003-0787-298X https://orcid.org/0000-0002-4267-237X |
| _version_ | 1826198002036899840 |
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| author | Moon, James J. Suh, Heikyung Bershteyn, Anna Stephan, Matthias T. Liu, Haipeng Huang, Bonnie Sohail, Mashaal Luo, Samantha Ho Um, Soong Khant, Htet Goodwin, Jessica T. Ramos, Jenelyn Chiu, Wah Irvine, Darrell J |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Moon, James J. Suh, Heikyung Bershteyn, Anna Stephan, Matthias T. Liu, Haipeng Huang, Bonnie Sohail, Mashaal Luo, Samantha Ho Um, Soong Khant, Htet Goodwin, Jessica T. Ramos, Jenelyn Chiu, Wah Irvine, Darrell J |
| author_sort | Moon, James J. |
| collection | MIT |
| description | available in PMC 2011 September 1 |
| first_indexed | 2024-09-23T10:57:20Z |
| format | Article |
| id | mit-1721.1/79763 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:57:20Z |
| publishDate | 2013 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/797632022-09-27T16:07:59Z Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses Moon, James J. Suh, Heikyung Bershteyn, Anna Stephan, Matthias T. Liu, Haipeng Huang, Bonnie Sohail, Mashaal Luo, Samantha Ho Um, Soong Khant, Htet Goodwin, Jessica T. Ramos, Jenelyn Chiu, Wah Irvine, Darrell J Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Ragon Institute of MGH, MIT and Harvard Koch Institute for Integrative Cancer Research at MIT Moon, James J. Suh, Heikyung Bershteyn, Anna Stephan, Matthias T. Liu, Haipeng Huang, Bonnie Sohail, Mashaal Luo, Samantha Ho Um, Soong Irvine, Darrell J. available in PMC 2011 September 1 Vaccines based on recombinant proteins avoid the toxicity and antivector immunity associated with live vaccine (for example, viral) vectors, but their immunogenicity is poor, particularly for CD8+ T-cell responses. Synthetic particles carrying antigens and adjuvant molecules have been developed to enhance subunit vaccines, but in general these materials have failed to elicit CD8+ T-cell responses comparable to those for live vectors in preclinical animal models. Here, we describe interbilayer-crosslinked multilamellar vesicles formed by crosslinking headgroups of adjacent lipid bilayers within multilamellar vesicles. Interbilayer-crosslinked vesicles stably entrapped protein antigens in the vesicle core and lipid-based immunostimulatory molecules in the vesicle walls under extracellular conditions, but exhibited rapid release in the presence of endolysosomal lipases. We found that these antigen/adjuvant-carrying vesicles form an extremely potent whole-protein vaccine, eliciting endogenous T-cell and antibody responses comparable to those for the strongest vaccine vectors. These materials should enable a range of subunit vaccines and provide new possibilities for therapeutic protein delivery. Ragon Institute of MGH, MIT and Harvard Bill & Melinda Gates Foundation United States. Dept. of Defense (contract W911NF-07-D-0004) National Institutes of Health (U.S.) (P41RR002250) National Institutes of Health (U.S.) (RC2GM092599) 2013-08-01T20:40:16Z 2013-08-01T20:40:16Z 2011-02 Article http://purl.org/eprint/type/JournalArticle 1476-1122 1476-4660 http://hdl.handle.net/1721.1/79763 Moon, James J., Heikyung Suh, Anna Bershteyn, Matthias T. Stephan, Haipeng Liu, Bonnie Huang, Mashaal Sohail, et al. “Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses.” Nature Materials 10, no. 3 (February 20, 2011): 243-251. https://orcid.org/0000-0003-0787-298X https://orcid.org/0000-0002-4267-237X en_US http://dx.doi.org/10.1038/nmat2960 Nature Materials Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PubMed Central |
| spellingShingle | Moon, James J. Suh, Heikyung Bershteyn, Anna Stephan, Matthias T. Liu, Haipeng Huang, Bonnie Sohail, Mashaal Luo, Samantha Ho Um, Soong Khant, Htet Goodwin, Jessica T. Ramos, Jenelyn Chiu, Wah Irvine, Darrell J Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses |
| title | Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses |
| title_full | Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses |
| title_fullStr | Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses |
| title_full_unstemmed | Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses |
| title_short | Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses |
| title_sort | interbilayer crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses |
| url | http://hdl.handle.net/1721.1/79763 https://orcid.org/0000-0003-0787-298X https://orcid.org/0000-0002-4267-237X |
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