350-μm side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus
Miniature endoscopic probes offer a solution for deep brain imaging by overcoming the limited depth of intravital microscopy. We describe a small-diameter (350 μm) graded-index optical probe with a side-view design for in vivo cellular imaging of the mammalian brain. The side-view probe provides a u...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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SPIE
2013
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| Online Access: | http://hdl.handle.net/1721.1/80328 |
| _version_ | 1811090675557466112 |
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| author | Kim, Jun Ki Yun, Seok Hyun (Andy) Choi, Jin Woo |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Kim, Jun Ki Yun, Seok Hyun (Andy) Choi, Jin Woo |
| author_sort | Kim, Jun Ki |
| collection | MIT |
| description | Miniature endoscopic probes offer a solution for deep brain imaging by overcoming the limited depth of intravital microscopy. We describe a small-diameter (350 μm) graded-index optical probe with a side-view design for in vivo cellular imaging of the mammalian brain. The side-view probe provides a unique view of the vertical network of neurons and penetrating blood vessels. At a given insertion site, the translational and rotational scanning of the probe provides access to a large tissue area (>1 mm[superscript 2]) across the cortex, hippocampus, thalamus, and hypothalamus. |
| first_indexed | 2024-09-23T14:50:05Z |
| format | Article |
| id | mit-1721.1/80328 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:50:05Z |
| publishDate | 2013 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/803282022-10-01T22:47:02Z 350-μm side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus Kim, Jun Ki Yun, Seok Hyun (Andy) Choi, Jin Woo Harvard University--MIT Division of Health Sciences and Technology Yun, Seok Hyun (Andy) Miniature endoscopic probes offer a solution for deep brain imaging by overcoming the limited depth of intravital microscopy. We describe a small-diameter (350 μm) graded-index optical probe with a side-view design for in vivo cellular imaging of the mammalian brain. The side-view probe provides a unique view of the vertical network of neurons and penetrating blood vessels. At a given insertion site, the translational and rotational scanning of the probe provides access to a large tissue area (>1 mm[superscript 2]) across the cortex, hippocampus, thalamus, and hypothalamus. United States. Dept. of Defense (Grant FA9550-10-1-0537) 2013-08-30T15:04:18Z 2013-08-30T15:04:18Z 2013-04 2013-03 Article http://purl.org/eprint/type/JournalArticle 1083-3668 1560-2281 http://hdl.handle.net/1721.1/80328 Kim, Jun Ki. “350-<italic>μ</italic>m side-view optical probe for imaging the murine brain <italic>in vivo</italic> from the cortex to the hypothalamus.” Journal of Biomedical Optics 18, no. 5 (May 1, 2013): 050502. © 2013 Society of Photo-Optical Instrumentation Engineers en_US http://dx.doi.org/10.1117/1.JBO.18.5.050502 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Kim, Jun Ki Yun, Seok Hyun (Andy) Choi, Jin Woo 350-μm side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus |
| title | 350-μm side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus |
| title_full | 350-μm side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus |
| title_fullStr | 350-μm side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus |
| title_full_unstemmed | 350-μm side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus |
| title_short | 350-μm side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus |
| title_sort | 350 μm side view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus |
| url | http://hdl.handle.net/1721.1/80328 |
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