Long noncoding RNAs regulate adipogenesis
The prevalence of obesity has led to a surge of interest in understanding the detailed mechanisms underlying adipocyte development. Many protein-coding genes, mRNAs, and microRNAs have been implicated in adipocyte development, but the global expression patterns and functional contributions of long n...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
National Academy of Sciences (U.S.)
2013
|
| Online Access: | http://hdl.handle.net/1721.1/80723 https://orcid.org/0000-0002-7029-7415 |
| _version_ | 1811069026391031808 |
|---|---|
| author | Sun, Lei Goff, Loyal Alexander, Ryan K. Lo, Kinyui Alice Yuan, Bingbing B. Kellis, Manolis Lodish, Harvey F. Trapnell, Cole Hacisuleyman, Ezgi Sauvageau, Martin Tazon-Vega, Barbara Kelley, David R. Hendrickson, David G. Rinn, John L. |
| author2 | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory |
| author_facet | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Sun, Lei Goff, Loyal Alexander, Ryan K. Lo, Kinyui Alice Yuan, Bingbing B. Kellis, Manolis Lodish, Harvey F. Trapnell, Cole Hacisuleyman, Ezgi Sauvageau, Martin Tazon-Vega, Barbara Kelley, David R. Hendrickson, David G. Rinn, John L. |
| author_sort | Sun, Lei |
| collection | MIT |
| description | The prevalence of obesity has led to a surge of interest in understanding the detailed mechanisms underlying adipocyte development. Many protein-coding genes, mRNAs, and microRNAs have been implicated in adipocyte development, but the global expression patterns and functional contributions of long noncoding RNA (lncRNA) during adipogenesis have not been explored. Here we profiled the transcriptome of primary brown and white adipocytes, preadipocytes, and cultured adipocytes and identified 175 lncRNAs that are specifically regulated during adipogenesis. Many lncRNAs are adipose-enriched, strongly induced during adipogenesis, and bound at their promoters by key transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (CEBPα). RNAi-mediated loss of function screens identified functional lncRNAs with varying impact on adipogenesis. Collectively, we have identified numerous lncRNAs that are functionally required for proper adipogenesis. |
| first_indexed | 2024-09-23T08:04:31Z |
| format | Article |
| id | mit-1721.1/80723 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:04:31Z |
| publishDate | 2013 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/807232022-09-30T07:17:32Z Long noncoding RNAs regulate adipogenesis Sun, Lei Goff, Loyal Alexander, Ryan K. Lo, Kinyui Alice Yuan, Bingbing B. Kellis, Manolis Lodish, Harvey F. Trapnell, Cole Hacisuleyman, Ezgi Sauvageau, Martin Tazon-Vega, Barbara Kelley, David R. Hendrickson, David G. Rinn, John L. Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Whitehead Institute for Biomedical Research Sun, Lei Goff, Loyal Alexander, Ryan K. Lo, Kinyui Alice Yuan, Bingbing B. Kellis, Manolis Lodish, Harvey F. The prevalence of obesity has led to a surge of interest in understanding the detailed mechanisms underlying adipocyte development. Many protein-coding genes, mRNAs, and microRNAs have been implicated in adipocyte development, but the global expression patterns and functional contributions of long noncoding RNA (lncRNA) during adipogenesis have not been explored. Here we profiled the transcriptome of primary brown and white adipocytes, preadipocytes, and cultured adipocytes and identified 175 lncRNAs that are specifically regulated during adipogenesis. Many lncRNAs are adipose-enriched, strongly induced during adipogenesis, and bound at their promoters by key transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (CEBPα). RNAi-mediated loss of function screens identified functional lncRNAs with varying impact on adipogenesis. Collectively, we have identified numerous lncRNAs that are functionally required for proper adipogenesis. National Institutes of Health (U.S.). (Grant DK047618) National Institutes of Health (U.S.). (Grant DK068348) National Institutes of Health (U.S.). (Grant 5P01HL066105) National Science Foundation (U.S.) (Postdoctoral Fellowship) 2013-09-13T16:04:49Z 2013-09-13T16:04:49Z 2013-02 2012-10 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/80723 Sun, L., L. A. Goff, C. Trapnell, R. Alexander, K. A. Lo, E. Hacisuleyman, M. Sauvageau, et al. “Long noncoding RNAs regulate adipogenesis.” Proceedings of the National Academy of Sciences 110, no. 9 (February 26, 2013): 3387-3392. https://orcid.org/0000-0002-7029-7415 en_US http://dx.doi.org/10.1073/pnas.1222643110 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Sun, Lei Goff, Loyal Alexander, Ryan K. Lo, Kinyui Alice Yuan, Bingbing B. Kellis, Manolis Lodish, Harvey F. Trapnell, Cole Hacisuleyman, Ezgi Sauvageau, Martin Tazon-Vega, Barbara Kelley, David R. Hendrickson, David G. Rinn, John L. Long noncoding RNAs regulate adipogenesis |
| title | Long noncoding RNAs regulate adipogenesis |
| title_full | Long noncoding RNAs regulate adipogenesis |
| title_fullStr | Long noncoding RNAs regulate adipogenesis |
| title_full_unstemmed | Long noncoding RNAs regulate adipogenesis |
| title_short | Long noncoding RNAs regulate adipogenesis |
| title_sort | long noncoding rnas regulate adipogenesis |
| url | http://hdl.handle.net/1721.1/80723 https://orcid.org/0000-0002-7029-7415 |
| work_keys_str_mv | AT sunlei longnoncodingrnasregulateadipogenesis AT goffloyal longnoncodingrnasregulateadipogenesis AT alexanderryank longnoncodingrnasregulateadipogenesis AT lokinyuialice longnoncodingrnasregulateadipogenesis AT yuanbingbingb longnoncodingrnasregulateadipogenesis AT kellismanolis longnoncodingrnasregulateadipogenesis AT lodishharveyf longnoncodingrnasregulateadipogenesis AT trapnellcole longnoncodingrnasregulateadipogenesis AT hacisuleymanezgi longnoncodingrnasregulateadipogenesis AT sauvageaumartin longnoncodingrnasregulateadipogenesis AT tazonvegabarbara longnoncodingrnasregulateadipogenesis AT kelleydavidr longnoncodingrnasregulateadipogenesis AT hendricksondavidg longnoncodingrnasregulateadipogenesis AT rinnjohnl longnoncodingrnasregulateadipogenesis |