Gene expression profiles and transcriptional regulatory pathways underlying mouse tissue macrophage identity and diversity
We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2013
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| Online Access: | http://hdl.handle.net/1721.1/80773 https://orcid.org/0000-0001-8567-2049 |
| _version_ | 1811068484946231296 |
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| author | Regev, Aviv Immunological Genome Project Consortium |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Regev, Aviv Immunological Genome Project Consortium |
| author_sort | Regev, Aviv |
| collection | MIT |
| description | We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were not present in all macrophages. Nonetheless, well-characterized surface markers, including MerTK and FcγR1 (CD64), along with a cluster of previously unidentified transcripts, were distinctly and universally associated with mature tissue macrophages. TCEF3, C/EBP-α, Bach1 and CREG-1 were among the transcriptional regulators predicted to regulate these core macrophage-associated genes. The mRNA encoding other transcription factors, such as Gata6, was associated with single macrophage populations. We further identified how these transcripts and the proteins they encode facilitated distinguishing macrophages from dendritic cells. |
| first_indexed | 2024-09-23T07:56:40Z |
| format | Article |
| id | mit-1721.1/80773 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T07:56:40Z |
| publishDate | 2013 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/807732022-09-23T09:46:35Z Gene expression profiles and transcriptional regulatory pathways underlying mouse tissue macrophage identity and diversity Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages Regev, Aviv Immunological Genome Project Consortium Massachusetts Institute of Technology. Department of Biology Regev, Aviv Regev, Aviv We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were not present in all macrophages. Nonetheless, well-characterized surface markers, including MerTK and FcγR1 (CD64), along with a cluster of previously unidentified transcripts, were distinctly and universally associated with mature tissue macrophages. TCEF3, C/EBP-α, Bach1 and CREG-1 were among the transcriptional regulators predicted to regulate these core macrophage-associated genes. The mRNA encoding other transcription factors, such as Gata6, was associated with single macrophage populations. We further identified how these transcripts and the proteins they encode facilitated distinguishing macrophages from dendritic cells. National Institute of Allergy and Infectious Diseases (U.S.) (R24 AI072073 to fund the ImmGen Project) National Institutes of Health (U.S.) (R01AI049653) National Institutes of Health (U.S.) (R01AI061741) National Institutes of Health (U.S.) (P50GM071558-03) National Institutes of Health (U.S.) (R01DK08854) National Institutes of Health (U.S.) (5T32DA007135-27) American Heart Association (10POST4160140) 2013-09-17T15:45:38Z 2013-09-17T15:45:38Z 2012-09 2012-04 Article http://purl.org/eprint/type/JournalArticle 1529-2908 1529-2916 http://hdl.handle.net/1721.1/80773 Gautier, Emmanuel L, Tal Shay, Jennifer Miller, Melanie Greter, Claudia Jakubzick, Stoyan Ivanov, Julie Helft, et al. “Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages.” Nature Immunology 13, no. 11 (September 30, 2012): 1118-1128. https://orcid.org/0000-0001-8567-2049 en_US http://dx.doi.org/10.1038/ni.2419 Nature Immunology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Nature Publishing Group Regev via Courtney Crummett |
| spellingShingle | Regev, Aviv Immunological Genome Project Consortium Gene expression profiles and transcriptional regulatory pathways underlying mouse tissue macrophage identity and diversity |
| title | Gene expression profiles and transcriptional regulatory pathways underlying mouse tissue macrophage identity and diversity |
| title_full | Gene expression profiles and transcriptional regulatory pathways underlying mouse tissue macrophage identity and diversity |
| title_fullStr | Gene expression profiles and transcriptional regulatory pathways underlying mouse tissue macrophage identity and diversity |
| title_full_unstemmed | Gene expression profiles and transcriptional regulatory pathways underlying mouse tissue macrophage identity and diversity |
| title_short | Gene expression profiles and transcriptional regulatory pathways underlying mouse tissue macrophage identity and diversity |
| title_sort | gene expression profiles and transcriptional regulatory pathways underlying mouse tissue macrophage identity and diversity |
| url | http://hdl.handle.net/1721.1/80773 https://orcid.org/0000-0001-8567-2049 |
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