Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications
Microcalcifications geographically target the location of abnormalities within the breast and are of critical importance in breast cancer diagnosis. However, despite stereotactic guidance, core needle biopsy fails to retrieve microcalcifications in up to 15% of patients. Here, we introduce an approa...
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2013
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| Online Access: | http://hdl.handle.net/1721.1/81206 |
| _version_ | 1826189312317718528 |
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| author | Soares, Jaqueline S. Barman, Ishan Dingari, Narahara Chari Volynskaya, Zoya Liu, Wendy Klein, Nina Plecha, Donna Dasari, Ramachandra Rao Fitzmaurice, Maryann Volynskaya, Zoya |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Soares, Jaqueline S. Barman, Ishan Dingari, Narahara Chari Volynskaya, Zoya Liu, Wendy Klein, Nina Plecha, Donna Dasari, Ramachandra Rao Fitzmaurice, Maryann Volynskaya, Zoya |
| author_sort | Soares, Jaqueline S. |
| collection | MIT |
| description | Microcalcifications geographically target the location of abnormalities within the breast and are of critical importance in breast cancer diagnosis. However, despite stereotactic guidance, core needle biopsy fails to retrieve microcalcifications in up to 15% of patients. Here, we introduce an approach based on diffuse reflectance spectroscopy for detection of microcalcifications that focuses on variations in optical absorption stemming from the calcified clusters and the associated cross-linking molecules. In this study, diffuse reflectance spectra are acquired ex vivo from 203 sites in fresh biopsy tissue cores from 23 patients undergoing stereotactic breast needle biopsies. By correlating the spectra with the corresponding radiographic and histologic assessment, we have developed a support vector machine-derived decision algorithm, which shows high diagnostic power (positive predictive value and negative predictive value of 97% and 88%, respectively) for diagnosis of lesions with microcalcifications. We further show that these results are robust and not due to any spurious correlations. We attribute our findings to the presence of proteins (such as elastin), and desmosine and isodesmosine cross-linkers in the microcalcifications. It is important to note that the performance of the diffuse reflectance decision algorithm is comparable to one derived from the corresponding Raman spectra, and the considerably higher intensity of the reflectance signal enables the detection of the targeted lesions in a fraction of the spectral acquisition time. Our findings create a unique landscape for spectroscopic validation of breast core needle biopsy for detection of microcalcifications that can substantially improve the likelihood of an adequate, diagnostic biopsy in the first attempt. |
| first_indexed | 2024-09-23T08:12:47Z |
| format | Article |
| id | mit-1721.1/81206 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:12:47Z |
| publishDate | 2013 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/812062022-09-30T08:19:39Z Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications Soares, Jaqueline S. Barman, Ishan Dingari, Narahara Chari Volynskaya, Zoya Liu, Wendy Klein, Nina Plecha, Donna Dasari, Ramachandra Rao Fitzmaurice, Maryann Volynskaya, Zoya Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Spectroscopy Laboratory Soares, Jaqueline S. Barman, Ishan Dingari, Narahara Chari Volynskaya, Zoya Dasari, Ramachandra Rao Microcalcifications geographically target the location of abnormalities within the breast and are of critical importance in breast cancer diagnosis. However, despite stereotactic guidance, core needle biopsy fails to retrieve microcalcifications in up to 15% of patients. Here, we introduce an approach based on diffuse reflectance spectroscopy for detection of microcalcifications that focuses on variations in optical absorption stemming from the calcified clusters and the associated cross-linking molecules. In this study, diffuse reflectance spectra are acquired ex vivo from 203 sites in fresh biopsy tissue cores from 23 patients undergoing stereotactic breast needle biopsies. By correlating the spectra with the corresponding radiographic and histologic assessment, we have developed a support vector machine-derived decision algorithm, which shows high diagnostic power (positive predictive value and negative predictive value of 97% and 88%, respectively) for diagnosis of lesions with microcalcifications. We further show that these results are robust and not due to any spurious correlations. We attribute our findings to the presence of proteins (such as elastin), and desmosine and isodesmosine cross-linkers in the microcalcifications. It is important to note that the performance of the diffuse reflectance decision algorithm is comparable to one derived from the corresponding Raman spectra, and the considerably higher intensity of the reflectance signal enables the detection of the targeted lesions in a fraction of the spectral acquisition time. Our findings create a unique landscape for spectroscopic validation of breast core needle biopsy for detection of microcalcifications that can substantially improve the likelihood of an adequate, diagnostic biopsy in the first attempt. National Center for Research Resources (U.S.) (P41-RR02594) National Institutes of Health (U.S.) National Cancer Institute (U.S.) (R01-CA140288). Conselho Nacional de Pesquisas (Brazil) 2013-09-26T20:21:44Z 2013-09-26T20:21:44Z 2012-12 2012-09 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/81206 Soares, J. S., I. Barman, N. C. Dingari, Z. Volynskaya, W. Liu, N. Klein, D. Plecha, R. R. Dasari, and M. Fitzmaurice. “Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications.” Proceedings of the National Academy of Sciences 110, no. 2 (January 8, 2013): 471-476.Copyright © 2012 National Academy of Sciences. en_US http://dx.doi.org/10.1073/pnas.1215473110 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Soares, Jaqueline S. Barman, Ishan Dingari, Narahara Chari Volynskaya, Zoya Liu, Wendy Klein, Nina Plecha, Donna Dasari, Ramachandra Rao Fitzmaurice, Maryann Volynskaya, Zoya Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications |
| title | Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications |
| title_full | Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications |
| title_fullStr | Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications |
| title_full_unstemmed | Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications |
| title_short | Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications |
| title_sort | diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications |
| url | http://hdl.handle.net/1721.1/81206 |
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