The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa Biofilms
Bacteria within biofilms secrete and surround themselves with an extracellular matrix, which serves as a first line of defense against antibiotic attack. Polysaccharides constitute major elements of the biofilm matrix and are implied in surface adhesion and biofilm organization, but their contributi...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2013
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| Online Access: | http://hdl.handle.net/1721.1/81214 https://orcid.org/0000-0002-4593-5606 https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-3199-0508 |
| _version_ | 1811096883897040896 |
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| author | Ramirez Millan, Maria Caldara, Marina Rusconi, Roberto Tarasova, Yekaterina Stocker, Roman Ribbeck, Katharina Billings, Amanda Nicole |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Ramirez Millan, Maria Caldara, Marina Rusconi, Roberto Tarasova, Yekaterina Stocker, Roman Ribbeck, Katharina Billings, Amanda Nicole |
| author_sort | Ramirez Millan, Maria |
| collection | MIT |
| description | Bacteria within biofilms secrete and surround themselves with an extracellular matrix, which serves as a first line of defense against antibiotic attack. Polysaccharides constitute major elements of the biofilm matrix and are implied in surface adhesion and biofilm organization, but their contributions to the resistance properties of biofilms remain largely elusive. Using a combination of static and continuous-flow biofilm experiments we show that Psl, one major polysaccharide in the Pseudomonas aeruginosa biofilm matrix, provides a generic first line of defense toward antibiotics with diverse biochemical properties during the initial stages of biofilm development. Furthermore, we show with mixed-strain experiments that antibiotic-sensitive “non-producing” cells lacking Psl can gain tolerance by integrating into Psl-containing biofilms. However, non-producers dilute the protective capacity of the matrix and hence, excessive incorporation can result in the collapse of resistance of the entire community. Our data also reveal that Psl mediated protection is extendible to E. coli and S. aureus in co-culture biofilms. Together, our study shows that Psl represents a critical first bottleneck to the antibiotic attack of a biofilm community early in biofilm development. |
| first_indexed | 2024-09-23T16:50:53Z |
| format | Article |
| id | mit-1721.1/81214 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:50:53Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/812142022-10-03T08:40:12Z The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa Biofilms Ramirez Millan, Maria Caldara, Marina Rusconi, Roberto Tarasova, Yekaterina Stocker, Roman Ribbeck, Katharina Billings, Amanda Nicole Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Billings, Amanda Nicole Ramirez Millan, Maria Caldara, Marina Tarasova, Yekaterina Ribbeck, Katharina Rusconi, Roberto Stocker, Roman Bacteria within biofilms secrete and surround themselves with an extracellular matrix, which serves as a first line of defense against antibiotic attack. Polysaccharides constitute major elements of the biofilm matrix and are implied in surface adhesion and biofilm organization, but their contributions to the resistance properties of biofilms remain largely elusive. Using a combination of static and continuous-flow biofilm experiments we show that Psl, one major polysaccharide in the Pseudomonas aeruginosa biofilm matrix, provides a generic first line of defense toward antibiotics with diverse biochemical properties during the initial stages of biofilm development. Furthermore, we show with mixed-strain experiments that antibiotic-sensitive “non-producing” cells lacking Psl can gain tolerance by integrating into Psl-containing biofilms. However, non-producers dilute the protective capacity of the matrix and hence, excessive incorporation can result in the collapse of resistance of the entire community. Our data also reveal that Psl mediated protection is extendible to E. coli and S. aureus in co-culture biofilms. Together, our study shows that Psl represents a critical first bottleneck to the antibiotic attack of a biofilm community early in biofilm development. National Institutes of Health (U.S.). National Institute of Environmental Health Sciences (Training Grant in Toxicology 5 T32 ES7020-37) 2013-09-27T15:11:05Z 2013-09-27T15:11:05Z 2013-08 2012-11 Article http://purl.org/eprint/type/JournalArticle 1553-7374 1553-7366 http://hdl.handle.net/1721.1/81214 Billings, Nicole, Maria Ramirez Millan, Marina Caldara, Roberto Rusconi, Yekaterina Tarasova, Roman Stocker, and Katharina Ribbeck. “The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa Biofilms.” Edited by Matthew R. Parsek. PLoS Pathogens 9, no. 8 (August 8, 2013): e1003526. https://orcid.org/0000-0002-4593-5606 https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-3199-0508 en_US http://dx.doi.org/10.1371/journal.ppat.1003526 PLoS Pathogens Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Ramirez Millan, Maria Caldara, Marina Rusconi, Roberto Tarasova, Yekaterina Stocker, Roman Ribbeck, Katharina Billings, Amanda Nicole The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa Biofilms |
| title | The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa Biofilms |
| title_full | The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa Biofilms |
| title_fullStr | The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa Biofilms |
| title_full_unstemmed | The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa Biofilms |
| title_short | The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa Biofilms |
| title_sort | extracellular matrix component psl provides fast acting antibiotic defense in pseudomonas aeruginosa biofilms |
| url | http://hdl.handle.net/1721.1/81214 https://orcid.org/0000-0002-4593-5606 https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-3199-0508 |
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