Selective Light-Triggered Release of DNA from Gold Nanorods Switches Blood Clotting On and Off
Blood clotting is a precise cascade engineered to form a clot with temporal and spatial control. Current control of blood clotting is achieved predominantly by anticoagulants and thus inherently one-sided. Here we use a pair of nanorods (NRs) to provide a two-way switch for the blood clotting cascad...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2013
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| Online Access: | http://hdl.handle.net/1721.1/81232 https://orcid.org/0000-0002-5368-6996 https://orcid.org/0000-0002-9478-2239 |
| _version_ | 1811082144301187072 |
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| author | Cifuentes Rius, Anna Baxamusa, Salmaan H. de Puig Guixe, Helena Flemister, Dorma C. Hamad-Schifferli, Kimberly |
| author2 | Lincoln Laboratory |
| author_facet | Lincoln Laboratory Cifuentes Rius, Anna Baxamusa, Salmaan H. de Puig Guixe, Helena Flemister, Dorma C. Hamad-Schifferli, Kimberly |
| author_sort | Cifuentes Rius, Anna |
| collection | MIT |
| description | Blood clotting is a precise cascade engineered to form a clot with temporal and spatial control. Current control of blood clotting is achieved predominantly by anticoagulants and thus inherently one-sided. Here we use a pair of nanorods (NRs) to provide a two-way switch for the blood clotting cascade by utilizing their ability to selectively release species on their surface under two different laser excitations. We selectively trigger release of a thrombin binding aptamer from one nanorod, inhibiting blood clotting and resulting in increased clotting time. We then release the complementary DNA as an antidote from the other NR, reversing the effect of the aptamer and restoring blood clotting. Thus, the nanorod pair acts as an on/off switch. One challenge for nanobiotechnology is the bio-nano interface, where coronas of weakly adsorbed proteins can obscure biomolecular function. We exploit these adsorbed proteins to increase aptamer and antidote loading on the nanorods. |
| first_indexed | 2024-09-23T11:58:16Z |
| format | Article |
| id | mit-1721.1/81232 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:58:16Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/812322022-10-01T07:20:39Z Selective Light-Triggered Release of DNA from Gold Nanorods Switches Blood Clotting On and Off Cifuentes Rius, Anna Baxamusa, Salmaan H. de Puig Guixe, Helena Flemister, Dorma C. Hamad-Schifferli, Kimberly Lincoln Laboratory Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Mechanical Engineering de Puig Guixe, Helena Hamad, Kimberly Cifuentes Rius, Anna Flemister, Dorma C. Baxamusa, Salmaan H. Blood clotting is a precise cascade engineered to form a clot with temporal and spatial control. Current control of blood clotting is achieved predominantly by anticoagulants and thus inherently one-sided. Here we use a pair of nanorods (NRs) to provide a two-way switch for the blood clotting cascade by utilizing their ability to selectively release species on their surface under two different laser excitations. We selectively trigger release of a thrombin binding aptamer from one nanorod, inhibiting blood clotting and resulting in increased clotting time. We then release the complementary DNA as an antidote from the other NR, reversing the effect of the aptamer and restoring blood clotting. Thus, the nanorod pair acts as an on/off switch. One challenge for nanobiotechnology is the bio-nano interface, where coronas of weakly adsorbed proteins can obscure biomolecular function. We exploit these adsorbed proteins to increase aptamer and antidote loading on the nanorods. National Science Foundation (U.S.) (Grant DMR #0906838) 2013-09-30T14:04:34Z 2013-09-30T14:04:34Z 2013-07 2013-05 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/81232 de Puig, Helena, Anna Cifuentes Rius, Dorma Flemister, Salmaan H. Baxamusa, and Kimberly Hamad-Schifferli. “Selective Light-Triggered Release of DNA from Gold Nanorods Switches Blood Clotting On and Off.” Edited by Maxim Antopolsky. PLoS ONE 8, no. 7 (July 24, 2013): e68511. https://orcid.org/0000-0002-5368-6996 https://orcid.org/0000-0002-9478-2239 en_US http://dx.doi.org/10.1371/journal.pone.0068511 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Cifuentes Rius, Anna Baxamusa, Salmaan H. de Puig Guixe, Helena Flemister, Dorma C. Hamad-Schifferli, Kimberly Selective Light-Triggered Release of DNA from Gold Nanorods Switches Blood Clotting On and Off |
| title | Selective Light-Triggered Release of DNA from Gold Nanorods Switches Blood Clotting On and Off |
| title_full | Selective Light-Triggered Release of DNA from Gold Nanorods Switches Blood Clotting On and Off |
| title_fullStr | Selective Light-Triggered Release of DNA from Gold Nanorods Switches Blood Clotting On and Off |
| title_full_unstemmed | Selective Light-Triggered Release of DNA from Gold Nanorods Switches Blood Clotting On and Off |
| title_short | Selective Light-Triggered Release of DNA from Gold Nanorods Switches Blood Clotting On and Off |
| title_sort | selective light triggered release of dna from gold nanorods switches blood clotting on and off |
| url | http://hdl.handle.net/1721.1/81232 https://orcid.org/0000-0002-5368-6996 https://orcid.org/0000-0002-9478-2239 |
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