tRNAs Marked with CCACCA Are Targeted for Degradation
Author Manuscript 2012 May 11
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Association for the Advancement of Science (AAAS)
2013
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| Online Access: | http://hdl.handle.net/1721.1/81255 https://orcid.org/0000-0003-1465-1691 |
| _version_ | 1811072045552762880 |
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| author | Whipple, J. M. Phizicky, E. M. Wilusz, Jeremy Sharp, Phillip A. |
| author2 | Koch Institute for Integrative Cancer Research at MIT |
| author_facet | Koch Institute for Integrative Cancer Research at MIT Whipple, J. M. Phizicky, E. M. Wilusz, Jeremy Sharp, Phillip A. |
| author_sort | Whipple, J. M. |
| collection | MIT |
| description | Author Manuscript 2012 May 11 |
| first_indexed | 2024-09-23T09:00:03Z |
| format | Article |
| id | mit-1721.1/81255 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:00:03Z |
| publishDate | 2013 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/812552022-09-26T09:44:49Z tRNAs Marked with CCACCA Are Targeted for Degradation Whipple, J. M. Phizicky, E. M. Wilusz, Jeremy Sharp, Phillip A. Koch Institute for Integrative Cancer Research at MIT Wilusz, Jeremy Sharp, Phillip A. Author Manuscript 2012 May 11 The CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase] adds CCA to the 3′ ends of transfer RNAs (tRNAs), a critical step in tRNA biogenesis that generates the amino acid attachment site. We found that the CCA-adding enzyme plays a key role in tRNA quality control by selectively marking structurally unstable tRNAs and tRNA-like small RNAs for degradation. Instead of adding CCA to the 3′ ends of these transcripts, CCA-adding enzymes from all three kingdoms of life add CCACCA. In addition, hypomodified mature tRNAs are subjected to CCACCA addition as part of a rapid tRNA decay pathway in vivo. We conjecture that CCACCA addition is a universal mechanism for controlling tRNA levels and preventing errors in translation. National Institutes of Health (U.S.) (Training Grant in Cellular, Biochemical and Molecular Sciences 5T32-GM068411) National Institutes of Health (U.S.) (Grant R01-GM34277) National Institutes of Health (U.S.) (Grant R01-CA133404) National Cancer Institute (U.S.) (Cancer Center Support (core) Grant P30-CA14051) 2013-10-01T13:50:24Z 2013-10-01T13:50:24Z 2011-11 2011-09 Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 http://hdl.handle.net/1721.1/81255 Wilusz, J. E., J. M. Whipple, E. M. Phizicky, and P. A. Sharp. “tRNAs Marked with CCACCA Are Targeted for Degradation.” Science 334, no. 6057 (November 10, 2011): 817-821. https://orcid.org/0000-0003-1465-1691 en_US http://dx.doi.org/10.1126/science.1213671 Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Association for the Advancement of Science (AAAS) PubMed Central |
| spellingShingle | Whipple, J. M. Phizicky, E. M. Wilusz, Jeremy Sharp, Phillip A. tRNAs Marked with CCACCA Are Targeted for Degradation |
| title | tRNAs Marked with CCACCA Are Targeted for Degradation |
| title_full | tRNAs Marked with CCACCA Are Targeted for Degradation |
| title_fullStr | tRNAs Marked with CCACCA Are Targeted for Degradation |
| title_full_unstemmed | tRNAs Marked with CCACCA Are Targeted for Degradation |
| title_short | tRNAs Marked with CCACCA Are Targeted for Degradation |
| title_sort | trnas marked with ccacca are targeted for degradation |
| url | http://hdl.handle.net/1721.1/81255 https://orcid.org/0000-0003-1465-1691 |
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