Massively Parallel Microfluidic Cell-Pairing Platform for the Statistical Study of Immunological Cell-Cell Interactions
Variability in cell-cell interactions is ubiquitous and particularly relevant for the immune system, where the reliability of cell-cell interactions is critical for the prevention of disease. This variability is poorly understood mainly due to the limitations of current methods. We have therefore de...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Chemical and Biological Microsystems Society
2013
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| Online Access: | http://hdl.handle.net/1721.1/81324 https://orcid.org/0000-0001-8898-2296 https://orcid.org/0000-0002-1090-6071 |
| Summary: | Variability in cell-cell interactions is ubiquitous and particularly relevant for the immune system, where the reliability of cell-cell interactions is critical for the prevention of disease. This variability is poorly understood mainly due to the limitations of current methods. We have therefore designed a highly parallel microfluidic cell-pairing device and optimized its pairing efficiency using fluids modeling. The optimized device can hydrodynamically pair hundreds of primary mouse immune-cells at an efficiency of ~50%. We measured T cell activation dynamics of ~130 primary mouse T cells paired with B cells. Our findings represent the first time that variation has been observed in T cell activation dynamics. |
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