Dosimetry of N[superscript 6]-Formyllysine Adducts Following [[superscript 13]C[superscript 2]H[subscript 2]]-Formaldehyde Exposures in Rats
With formaldehyde as the major source of endogenous N[superscript 6]-formyllysine protein adducts, we quantified endogenous and exogenous N[superscript 6]-formyllysine in the nasal epithelium of rats exposed by inhalation to 0.7, 2, 5.8, and 9.1 ppm [[superscript 13]C[superscript 2]H[subscript 2]]-f...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2013
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| Online Access: | http://hdl.handle.net/1721.1/82090 https://orcid.org/0000-0003-0011-3067 https://orcid.org/0000-0003-1230-9251 https://orcid.org/0000-0002-4607-5337 |
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| author | Edrissi, Bahar Taghizadeh, Koli Moeller, Benjamin C. Kracko, Dean Doyle-Eisele, Melanie Swenberg, James A. Dedon, Peter C. |
| author2 | Massachusetts Institute of Technology. Center for Environmental Health Sciences |
| author_facet | Massachusetts Institute of Technology. Center for Environmental Health Sciences Edrissi, Bahar Taghizadeh, Koli Moeller, Benjamin C. Kracko, Dean Doyle-Eisele, Melanie Swenberg, James A. Dedon, Peter C. |
| author_sort | Edrissi, Bahar |
| collection | MIT |
| description | With formaldehyde as the major source of endogenous N[superscript 6]-formyllysine protein adducts, we quantified endogenous and exogenous N[superscript 6]-formyllysine in the nasal epithelium of rats exposed by inhalation to 0.7, 2, 5.8, and 9.1 ppm [[superscript 13]C[superscript 2]H[subscript 2]]-formaldehyde using liquid chromatography-coupled tandem mass spectrometry. Exogenous N[superscript 6]-formyllysine was detected in the nasal epithelium, with concentration-dependent formation in total as well as fractionated (cytoplasmic, membrane, nuclear) proteins, but was not detected in the lung, liver, or bone marrow. Endogenous adducts dominated at all exposure conditions, with a 6 h 9.1 ppm formaldehyde exposure resulting in one-third of the total load of N[superscript 6]-formyllysine being derived from exogenous sources. The results parallel previous studies of formaldehyde-induced DNA adducts. |
| first_indexed | 2024-09-23T08:35:10Z |
| format | Article |
| id | mit-1721.1/82090 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:35:10Z |
| publishDate | 2013 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/820902022-09-30T09:46:05Z Dosimetry of N[superscript 6]-Formyllysine Adducts Following [[superscript 13]C[superscript 2]H[subscript 2]]-Formaldehyde Exposures in Rats Edrissi, Bahar Taghizadeh, Koli Moeller, Benjamin C. Kracko, Dean Doyle-Eisele, Melanie Swenberg, James A. Dedon, Peter C. Massachusetts Institute of Technology. Center for Environmental Health Sciences Massachusetts Institute of Technology. Department of Biological Engineering Edrissi, Bahar Taghizadeh, Koli Dedon, Peter C. With formaldehyde as the major source of endogenous N[superscript 6]-formyllysine protein adducts, we quantified endogenous and exogenous N[superscript 6]-formyllysine in the nasal epithelium of rats exposed by inhalation to 0.7, 2, 5.8, and 9.1 ppm [[superscript 13]C[superscript 2]H[subscript 2]]-formaldehyde using liquid chromatography-coupled tandem mass spectrometry. Exogenous N[superscript 6]-formyllysine was detected in the nasal epithelium, with concentration-dependent formation in total as well as fractionated (cytoplasmic, membrane, nuclear) proteins, but was not detected in the lung, liver, or bone marrow. Endogenous adducts dominated at all exposure conditions, with a 6 h 9.1 ppm formaldehyde exposure resulting in one-third of the total load of N[superscript 6]-formyllysine being derived from exogenous sources. The results parallel previous studies of formaldehyde-induced DNA adducts. David H. Koch Cancer Research Fund National Institutes of Health (U.S.) (National Institute of Environmental Health Sciences Grant ES016450) National Institutes of Health (U.S.) (National Institute of Environmental Health Sciences Grant ES005948) National Institutes of Health (U.S.) (National Institute of Environmental Health Sciences Grant ES010126) National Institutes of Health (U.S.) (National Institute of Environmental Health Sciences Grant ES002109) National Institutes of Health (U.S.) (National Cancer Institute (U.S.) Grant CA026731) National Institutes of Health (U.S.) (National Cancer Institute (U.S.) Grant CA103146) Texas. Commission of Environmental Quality 2013-11-12T17:05:44Z 2013-11-12T17:05:44Z 2013-10 2013-09 Article http://purl.org/eprint/type/JournalArticle 0893-228X 1520-5010 http://hdl.handle.net/1721.1/82090 Edrissi, Bahar, Koli Taghizadeh, Benjamin C. Moeller, Dean Kracko, Melanie Doyle-Eisele, James A. Swenberg, and Peter C. Dedon. “Dosimetry of N6-Formyllysine Adducts Following [13C2H2]-Formaldehyde Exposures in Rats.” Chemical Research in Toxicology 26, no. 10 (October 21, 2013): 1421-1423. © 2013 American Chemical Society https://orcid.org/0000-0003-0011-3067 https://orcid.org/0000-0003-1230-9251 https://orcid.org/0000-0002-4607-5337 en_US http://dx.doi.org/10.1021/tx400320u Chemical Research in Toxicology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Edrissi, Bahar Taghizadeh, Koli Moeller, Benjamin C. Kracko, Dean Doyle-Eisele, Melanie Swenberg, James A. Dedon, Peter C. Dosimetry of N[superscript 6]-Formyllysine Adducts Following [[superscript 13]C[superscript 2]H[subscript 2]]-Formaldehyde Exposures in Rats |
| title | Dosimetry of N[superscript 6]-Formyllysine Adducts Following [[superscript 13]C[superscript 2]H[subscript 2]]-Formaldehyde Exposures in Rats |
| title_full | Dosimetry of N[superscript 6]-Formyllysine Adducts Following [[superscript 13]C[superscript 2]H[subscript 2]]-Formaldehyde Exposures in Rats |
| title_fullStr | Dosimetry of N[superscript 6]-Formyllysine Adducts Following [[superscript 13]C[superscript 2]H[subscript 2]]-Formaldehyde Exposures in Rats |
| title_full_unstemmed | Dosimetry of N[superscript 6]-Formyllysine Adducts Following [[superscript 13]C[superscript 2]H[subscript 2]]-Formaldehyde Exposures in Rats |
| title_short | Dosimetry of N[superscript 6]-Formyllysine Adducts Following [[superscript 13]C[superscript 2]H[subscript 2]]-Formaldehyde Exposures in Rats |
| title_sort | dosimetry of n superscript 6 formyllysine adducts following superscript 13 c superscript 2 h subscript 2 formaldehyde exposures in rats |
| url | http://hdl.handle.net/1721.1/82090 https://orcid.org/0000-0003-0011-3067 https://orcid.org/0000-0003-1230-9251 https://orcid.org/0000-0002-4607-5337 |
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