CENTRIFUGAL LABTUBE FOR FULLY AUTOMATED DNA EXTRACTION & LAMP AMPLIFICATION BASED ON AN INTEGRATED, LOW-COST HEATING SYSTEM
In this paper, we introduce a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA-extraction platform (LabTube). We demonstrate fully automated, fully closed extraction of as little as 100 DNA copies of verotoxin-producing...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Transducers Research Foundations, Inc.
2013
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| Online Access: | http://hdl.handle.net/1721.1/82592 https://orcid.org/0000-0002-5048-4109 |
| Summary: | In this paper, we introduce a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA-extraction platform (LabTube). We demonstrate fully automated, fully closed extraction of as little as 100 DNA copies of verotoxin-producing (VTEC) Escherichia coli lysate in water, milk and apple juice in a standard laboratory centrifuge, followed by subsequent automatic LAMP amplification with an overall time-to-result of 1.5hrs. The system is disposable, fully closed and automated, requiring only a single pipetting step. The microcontroller-driven heating system is low-cost (<1$) and it can be easily parallelized. Because the heated LabSystem runs within a standard laboratory centrifuge, it is suitable for DNA extraction and amplification in low-resource areas, at production sites or sales locations. |
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