Probing the tumor microenvironment: collection and induction
The Nano Intravital Device, or NANIVID, is under development as an optically transparent, implantable tool to study the tumor microenvironment. Two etched glass substrates are sealed using a thin polymer membrane to create a reservoir with a single outlet. This reservoir is loaded with a hydrogel bl...
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| Format: | Article |
| Language: | en_US |
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SPIE
2014
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| Online Access: | http://hdl.handle.net/1721.1/83478 https://orcid.org/0000-0003-3214-4554 |
| _version_ | 1826207516622585856 |
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| author | Williams, James K. Padgen, Michael R. Wang, Yarong Entenberg, David Gertler, Frank Condeelis, John S. Castracane, James |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Williams, James K. Padgen, Michael R. Wang, Yarong Entenberg, David Gertler, Frank Condeelis, John S. Castracane, James |
| author_sort | Williams, James K. |
| collection | MIT |
| description | The Nano Intravital Device, or NANIVID, is under development as an optically transparent, implantable tool to study the tumor microenvironment. Two etched glass substrates are sealed using a thin polymer membrane to create a reservoir with a single outlet. This reservoir is loaded with a hydrogel blend that contains growth factors or other chemicals to be delivered to the tumor microenvironment. When the device is implanted in the tumor, the hydrogel will swell and release these entrapped molecules, forming a gradient. Validation of the device has been performed in vitro using epidermal growth factor (EGF) and Mena[subscript INV], a highly invasive, rat mammary adenocarcinoma cell line. In both 2-D and 3-D environments, cells migrated toward the gradient of EGF released from the device. The chorioallantoic membrane (CAM) of White Leghorn chicken eggs is being utilized to grow xenograft tumors that will be used for ex vivo cell collection. Device optimization is being performed for in vivo use as a tool to collect the invasive cell population. Preliminary cell collection experiments in vivo were performed using a mouse model of breast cancer. As a second application, the device is being explored as a delivery vehicle for chemicals that induce controlled changes in the tumor microenvironment. H[subscript 2]O[subscript 2] was loaded in the device and generated intracellular reactive oxygen species (ROS) in cells near the device outlet. In the future, other induction targets will be explored, including hypoglycemia and the manipulation of extracellular matrix stiffness. |
| first_indexed | 2024-09-23T13:50:47Z |
| format | Article |
| id | mit-1721.1/83478 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:50:47Z |
| publishDate | 2014 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/834782022-09-28T16:34:08Z Probing the tumor microenvironment: collection and induction Williams, James K. Padgen, Michael R. Wang, Yarong Entenberg, David Gertler, Frank Condeelis, John S. Castracane, James Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Gertler, Frank The Nano Intravital Device, or NANIVID, is under development as an optically transparent, implantable tool to study the tumor microenvironment. Two etched glass substrates are sealed using a thin polymer membrane to create a reservoir with a single outlet. This reservoir is loaded with a hydrogel blend that contains growth factors or other chemicals to be delivered to the tumor microenvironment. When the device is implanted in the tumor, the hydrogel will swell and release these entrapped molecules, forming a gradient. Validation of the device has been performed in vitro using epidermal growth factor (EGF) and Mena[subscript INV], a highly invasive, rat mammary adenocarcinoma cell line. In both 2-D and 3-D environments, cells migrated toward the gradient of EGF released from the device. The chorioallantoic membrane (CAM) of White Leghorn chicken eggs is being utilized to grow xenograft tumors that will be used for ex vivo cell collection. Device optimization is being performed for in vivo use as a tool to collect the invasive cell population. Preliminary cell collection experiments in vivo were performed using a mouse model of breast cancer. As a second application, the device is being explored as a delivery vehicle for chemicals that induce controlled changes in the tumor microenvironment. H[subscript 2]O[subscript 2] was loaded in the device and generated intracellular reactive oxygen species (ROS) in cells near the device outlet. In the future, other induction targets will be explored, including hypoglycemia and the manipulation of extracellular matrix stiffness. National Cancer Institute (U.S.) (Grant U54-CA126511-01) National Science Foundation (U.S.) (DBI0922830) 2014-01-06T15:06:06Z 2014-01-06T15:06:06Z 2012-02 2012-01 Article http://purl.org/eprint/type/ConferencePaper 0277-786X http://hdl.handle.net/1721.1/83478 Williams, James K., Michael R. Padgen, Yarong Wang, David Entenberg, Frank Gertler, John S. Condeelis, and James Castracane. “Probing the tumor microenvironment: collection and induction.” In Microfluidics, BioMEMS, and Medical Microsystems X, edited by Holger Becker and Bonnie L. Gray, 825105. © (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE) https://orcid.org/0000-0003-3214-4554 en_US http://dx.doi.org/10.1117/12.909045 Proceedings of SPIE--the International Society for Optical Engineering Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Williams, James K. Padgen, Michael R. Wang, Yarong Entenberg, David Gertler, Frank Condeelis, John S. Castracane, James Probing the tumor microenvironment: collection and induction |
| title | Probing the tumor microenvironment: collection and induction |
| title_full | Probing the tumor microenvironment: collection and induction |
| title_fullStr | Probing the tumor microenvironment: collection and induction |
| title_full_unstemmed | Probing the tumor microenvironment: collection and induction |
| title_short | Probing the tumor microenvironment: collection and induction |
| title_sort | probing the tumor microenvironment collection and induction |
| url | http://hdl.handle.net/1721.1/83478 https://orcid.org/0000-0003-3214-4554 |
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