Control of Development by Altered Localization of a Transcription Factor in B. subtilis
In B. subtilis, the chromosome partitioning proteins Soj (ParA) and Spo0J (ParB) regulate the initiation of sporulation. Soj is a negative regulator of sporulation gene expression, and Spo0J antagonizes Soj function. Using fusions of Soj to green fluorescent protein, we found that Soj localized near...
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/83847 https://orcid.org/0000-0002-8235-7227 |
| _version_ | 1826207858222432256 |
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| author | Lin, Daniel Chi-Hong Quisel, John D. Grossman, Alan Davis |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Lin, Daniel Chi-Hong Quisel, John D. Grossman, Alan Davis |
| author_sort | Lin, Daniel Chi-Hong |
| collection | MIT |
| description | In B. subtilis, the chromosome partitioning proteins Soj (ParA) and Spo0J (ParB) regulate the initiation of sporulation. Soj is a negative regulator of sporulation gene expression, and Spo0J antagonizes Soj function. Using fusions of Soj to green fluorescent protein, we found that Soj localized near the cell poles and upon entry into stationary phase oscillated from pole to pole. In the absence of Spo0J, Soj was associated predominantly with DNA. By in vivo cross-linking and immunoprecipitation, we found that Soj physically associates with developmentally regulated promoters, and this association increased in the absence of Spo0J. These results show that Soj switches localization and function depending on the chromosome partitioning protein Spo0J. We further show that mutations in the Soj ATPase domain disrupt localization and function and render Soj insensitive to regulation by Spo0J. |
| first_indexed | 2024-09-23T13:56:03Z |
| format | Article |
| id | mit-1721.1/83847 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:56:03Z |
| publishDate | 2014 |
| publisher | Elsevier |
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| spelling | mit-1721.1/838472022-10-01T18:05:13Z Control of Development by Altered Localization of a Transcription Factor in B. subtilis Lin, Daniel Chi-Hong Quisel, John D. Grossman, Alan Davis Massachusetts Institute of Technology. Department of Biology Quisel, John D. Lin, Daniel Chi-Hong Grossman, Alan D. In B. subtilis, the chromosome partitioning proteins Soj (ParA) and Spo0J (ParB) regulate the initiation of sporulation. Soj is a negative regulator of sporulation gene expression, and Spo0J antagonizes Soj function. Using fusions of Soj to green fluorescent protein, we found that Soj localized near the cell poles and upon entry into stationary phase oscillated from pole to pole. In the absence of Spo0J, Soj was associated predominantly with DNA. By in vivo cross-linking and immunoprecipitation, we found that Soj physically associates with developmentally regulated promoters, and this association increased in the absence of Spo0J. These results show that Soj switches localization and function depending on the chromosome partitioning protein Spo0J. We further show that mutations in the Soj ATPase domain disrupt localization and function and render Soj insensitive to regulation by Spo0J. National Science Foundation (U.S.) (Predoctoral Fellowship) National Institutes of Health (U.S.) (Public Health Services Grant GM41934) Merck/MIT Collaborative Program 2014-01-10T14:31:03Z 2014-01-10T14:31:03Z 1999-11 1999-08 Article http://purl.org/eprint/type/JournalArticle 10972765 1097-4164 http://hdl.handle.net/1721.1/83847 Quisel, John D, Daniel Chi-Hong Lin, and Alan D Grossman. “Control of Development by Altered Localization of a Transcription Factor in B. subtilis.” Molecular Cell 4, no. 5 (November 1999): 665-672. Copyright © 1999 Cell Press. https://orcid.org/0000-0002-8235-7227 en_US http://dx.doi.org/10.1016/S1097-2765(00)80377-9 Molecular Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier Open Archive |
| spellingShingle | Lin, Daniel Chi-Hong Quisel, John D. Grossman, Alan Davis Control of Development by Altered Localization of a Transcription Factor in B. subtilis |
| title | Control of Development by Altered Localization of a Transcription Factor in B. subtilis |
| title_full | Control of Development by Altered Localization of a Transcription Factor in B. subtilis |
| title_fullStr | Control of Development by Altered Localization of a Transcription Factor in B. subtilis |
| title_full_unstemmed | Control of Development by Altered Localization of a Transcription Factor in B. subtilis |
| title_short | Control of Development by Altered Localization of a Transcription Factor in B. subtilis |
| title_sort | control of development by altered localization of a transcription factor in b subtilis |
| url | http://hdl.handle.net/1721.1/83847 https://orcid.org/0000-0002-8235-7227 |
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