Visualization of Mismatch Repair in Bacterial Cells
We determined the localizations of mismatch repair proteins in living Bacillus subtilis cells. MutS-GFP colocalized with the chromosome in all cells and formed foci in a subset of cells. MutL-GFP formed foci in a subset of cells, and its localization was MutS dependent. The introduction of mismatche...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/83855 https://orcid.org/0000-0001-7243-8261 https://orcid.org/0000-0002-8235-7227 |
| _version_ | 1811096273611128832 |
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| author | Smith, Bradley T. Walker, Graham C. Grossman, Alan Davis |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Smith, Bradley T. Walker, Graham C. Grossman, Alan Davis |
| author_sort | Smith, Bradley T. |
| collection | MIT |
| description | We determined the localizations of mismatch repair proteins in living Bacillus subtilis cells. MutS-GFP colocalized with the chromosome in all cells and formed foci in a subset of cells. MutL-GFP formed foci in a subset of cells, and its localization was MutS dependent. The introduction of mismatches by growth in 2-aminopurine caused a replication-dependent increase in the number of cells with MutS and MutL foci. Approximately half of the MutS foci colocalized with DNA polymerase foci. We conclude that MutS is associated with the entire chromosome, poised to detect mismatches. After detection, it appears that mismatch repair foci assemble at mismatches as they emerge from the DNA polymerase and are then carried away from the replisome by continuing replication. |
| first_indexed | 2024-09-23T16:41:16Z |
| format | Article |
| id | mit-1721.1/83855 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:41:16Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/838552022-09-29T20:45:33Z Visualization of Mismatch Repair in Bacterial Cells Smith, Bradley T. Walker, Graham C. Grossman, Alan Davis Massachusetts Institute of Technology. Department of Biology Smith, Bradley T. Grossman, Alan D. Walker, Graham C. We determined the localizations of mismatch repair proteins in living Bacillus subtilis cells. MutS-GFP colocalized with the chromosome in all cells and formed foci in a subset of cells. MutL-GFP formed foci in a subset of cells, and its localization was MutS dependent. The introduction of mismatches by growth in 2-aminopurine caused a replication-dependent increase in the number of cells with MutS and MutL foci. Approximately half of the MutS foci colocalized with DNA polymerase foci. We conclude that MutS is associated with the entire chromosome, poised to detect mismatches. After detection, it appears that mismatch repair foci assemble at mismatches as they emerge from the DNA polymerase and are then carried away from the replisome by continuing replication. National Institutes of Health (U.S.) (Public Health Services Grant CA21615) National Institutes of Health (U.S.) (Public Health Services Grant GM41934) National Institutes of Health (U.S.) (Predoctoral Training Grant) 2014-01-10T15:36:53Z 2014-01-10T15:36:53Z 2001-12 2001-10 Article http://purl.org/eprint/type/JournalArticle 10972765 1097-4164 http://hdl.handle.net/1721.1/83855 Smith, Bradley T, Alan D Grossman, and Graham C Walker. “Visualization of Mismatch Repair in Bacterial Cells.” Molecular Cell 8.6 (2001): 1197–1206. Copyright © 2001 Cell Press https://orcid.org/0000-0001-7243-8261 https://orcid.org/0000-0002-8235-7227 en_US http://dx.doi.org/10.1016/S1097-2765(01)00402-6 Molecular Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier Open Archive |
| spellingShingle | Smith, Bradley T. Walker, Graham C. Grossman, Alan Davis Visualization of Mismatch Repair in Bacterial Cells |
| title | Visualization of Mismatch Repair in Bacterial Cells |
| title_full | Visualization of Mismatch Repair in Bacterial Cells |
| title_fullStr | Visualization of Mismatch Repair in Bacterial Cells |
| title_full_unstemmed | Visualization of Mismatch Repair in Bacterial Cells |
| title_short | Visualization of Mismatch Repair in Bacterial Cells |
| title_sort | visualization of mismatch repair in bacterial cells |
| url | http://hdl.handle.net/1721.1/83855 https://orcid.org/0000-0001-7243-8261 https://orcid.org/0000-0002-8235-7227 |
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