Diversity and Spatial Distribution of Hydrazine Oxidoreductase (hzo) Gene in the Oxygen Minimum Zone Off Costa Rica
Anaerobic ammonia oxidation (anammox) as an important nitrogen loss pathway has been reported in marine oxygen minimum zones (OMZs), but the community composition and spatial distribution of anammox bacteria in the eastern tropical North Pacific (ETNP) OMZ are poorly determined. In this study, anamm...
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Public Library of Science
2014
|
| Online Access: | http://hdl.handle.net/1721.1/83857 |
| _version_ | 1826191683126034432 |
|---|---|
| author | Kong, Liangliang Jing, Hongmei Kataoka, Takafumi Buchwald, Carolyn Liu, Hongbin |
| author2 | Woods Hole Oceanographic Institution |
| author_facet | Woods Hole Oceanographic Institution Kong, Liangliang Jing, Hongmei Kataoka, Takafumi Buchwald, Carolyn Liu, Hongbin |
| author_sort | Kong, Liangliang |
| collection | MIT |
| description | Anaerobic ammonia oxidation (anammox) as an important nitrogen loss pathway has been reported in marine oxygen minimum zones (OMZs), but the community composition and spatial distribution of anammox bacteria in the eastern tropical North Pacific (ETNP) OMZ are poorly determined. In this study, anammox bacterial communities in the OMZ off Costa Rica (CRD-OMZ) were analyzed based on both hydrazine oxidoreductase (hzo) genes and their transcripts assigned to cluster 1 and 2. The anammox communities revealed by hzo genes and proteins in CRD-OMZ showed a low diversity. Gene quantification results showed that hzo gene abundances peaked in the upper OMZs, associated with the peaks of nitrite concentration. Nitrite and oxygen concentrations may therefore colimit the distribution of anammox bacteria in this area. Furthermore, transcriptional activity of anammox bacteria was confirmed by obtaining abundant hzo mRNA transcripts through qRT-PCR. A novel hzo cluster 2x clade was identified by the phylogenetic analysis and these novel sequences were abundant and widely distributed in this environment. Our study demonstrated that both cluster 1 and 2 anammox bacteria play an active role in the CRD-OMZ, and the cluster 1 abundance and transcriptional activity were higher than cluster 2 in both free-living and particle-attached fractions at both gene and transcriptional levels. |
| first_indexed | 2024-09-23T08:59:43Z |
| format | Article |
| id | mit-1721.1/83857 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:59:43Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/838572022-09-26T09:42:23Z Diversity and Spatial Distribution of Hydrazine Oxidoreductase (hzo) Gene in the Oxygen Minimum Zone Off Costa Rica Kong, Liangliang Jing, Hongmei Kataoka, Takafumi Buchwald, Carolyn Liu, Hongbin Woods Hole Oceanographic Institution Buchwald, Carolyn Anaerobic ammonia oxidation (anammox) as an important nitrogen loss pathway has been reported in marine oxygen minimum zones (OMZs), but the community composition and spatial distribution of anammox bacteria in the eastern tropical North Pacific (ETNP) OMZ are poorly determined. In this study, anammox bacterial communities in the OMZ off Costa Rica (CRD-OMZ) were analyzed based on both hydrazine oxidoreductase (hzo) genes and their transcripts assigned to cluster 1 and 2. The anammox communities revealed by hzo genes and proteins in CRD-OMZ showed a low diversity. Gene quantification results showed that hzo gene abundances peaked in the upper OMZs, associated with the peaks of nitrite concentration. Nitrite and oxygen concentrations may therefore colimit the distribution of anammox bacteria in this area. Furthermore, transcriptional activity of anammox bacteria was confirmed by obtaining abundant hzo mRNA transcripts through qRT-PCR. A novel hzo cluster 2x clade was identified by the phylogenetic analysis and these novel sequences were abundant and widely distributed in this environment. Our study demonstrated that both cluster 1 and 2 anammox bacteria play an active role in the CRD-OMZ, and the cluster 1 abundance and transcriptional activity were higher than cluster 2 in both free-living and particle-attached fractions at both gene and transcriptional levels. National Science Foundation (U.S.) (OCE 096 1098) 2014-01-10T16:12:36Z 2014-01-10T16:12:36Z 2013-10 2013-06 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/83857 Kong, Liangliang, Hongmei Jing, Takafumi Kataoka, Carolyn Buchwald, and Hongbin Liu. “Diversity and Spatial Distribution of Hydrazine Oxidoreductase (hzo) Gene in the Oxygen Minimum Zone Off Costa Rica.” Edited by Zhi Zhou. PLoS ONE 8, no. 10 (October 31, 2013): e78275. en_US http://dx.doi.org/10.1371/journal.pone.0078275 PLoS ONE http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Kong, Liangliang Jing, Hongmei Kataoka, Takafumi Buchwald, Carolyn Liu, Hongbin Diversity and Spatial Distribution of Hydrazine Oxidoreductase (hzo) Gene in the Oxygen Minimum Zone Off Costa Rica |
| title | Diversity and Spatial Distribution of Hydrazine Oxidoreductase (hzo) Gene in the Oxygen Minimum Zone Off Costa Rica |
| title_full | Diversity and Spatial Distribution of Hydrazine Oxidoreductase (hzo) Gene in the Oxygen Minimum Zone Off Costa Rica |
| title_fullStr | Diversity and Spatial Distribution of Hydrazine Oxidoreductase (hzo) Gene in the Oxygen Minimum Zone Off Costa Rica |
| title_full_unstemmed | Diversity and Spatial Distribution of Hydrazine Oxidoreductase (hzo) Gene in the Oxygen Minimum Zone Off Costa Rica |
| title_short | Diversity and Spatial Distribution of Hydrazine Oxidoreductase (hzo) Gene in the Oxygen Minimum Zone Off Costa Rica |
| title_sort | diversity and spatial distribution of hydrazine oxidoreductase hzo gene in the oxygen minimum zone off costa rica |
| url | http://hdl.handle.net/1721.1/83857 |
| work_keys_str_mv | AT kongliangliang diversityandspatialdistributionofhydrazineoxidoreductasehzogeneintheoxygenminimumzoneoffcostarica AT jinghongmei diversityandspatialdistributionofhydrazineoxidoreductasehzogeneintheoxygenminimumzoneoffcostarica AT kataokatakafumi diversityandspatialdistributionofhydrazineoxidoreductasehzogeneintheoxygenminimumzoneoffcostarica AT buchwaldcarolyn diversityandspatialdistributionofhydrazineoxidoreductasehzogeneintheoxygenminimumzoneoffcostarica AT liuhongbin diversityandspatialdistributionofhydrazineoxidoreductasehzogeneintheoxygenminimumzoneoffcostarica |