Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy
The ability to deliver cells to appropriate target tissues is a prerequisite for successful cell-based therapy. To optimize cell therapy it is therefore necessary to develop a robust method of in vivo cell delivery quantification. Here we examine Mesenchymal Stem Cells (MSCs) labeled with a series o...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2014
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| Online Access: | http://hdl.handle.net/1721.1/83858 |
| _version_ | 1826196057619431424 |
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| author | Mortensen, Luke J. Levy, Oren Phillips, Joseph P. Stratton, Tara Triana, Brian Ruiz, Juan P. Gu, Fangqi Lin, Charles Karp, Jeffrey Michael |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Mortensen, Luke J. Levy, Oren Phillips, Joseph P. Stratton, Tara Triana, Brian Ruiz, Juan P. Gu, Fangqi Lin, Charles Karp, Jeffrey Michael |
| author_sort | Mortensen, Luke J. |
| collection | MIT |
| description | The ability to deliver cells to appropriate target tissues is a prerequisite for successful cell-based therapy. To optimize cell therapy it is therefore necessary to develop a robust method of in vivo cell delivery quantification. Here we examine Mesenchymal Stem Cells (MSCs) labeled with a series of 4 membrane dyes from which we select the optimal dye combination for pair-wise comparisons of delivery to inflamed tissue in the mouse ear using confocal fluorescence imaging. The use of an optimized dye pair for simultaneous tracking of two cell populations in the same animal enables quantification of a test population that is referenced to an internal control population, thereby eliminating intra-subject variations and variations in injected cell numbers. Consistent results were obtained even when the administered cell number varied by more than an order of magnitude, demonstrating an ability to neutralize one of the largest sources of in vivo experimental error and to greatly reduce the number of cells required to evaluate cell delivery. With this method, we are able to show a small but significant increase in the delivery of cytokine pre-treated MSCs (TNF-α & IFN-γ) compared to control MSCs. Our results suggest future directions for screening cell strategies using our in vivo cell delivery assay, which may be useful to develop methods to maximize cell therapeutic potential. |
| first_indexed | 2024-09-23T10:19:50Z |
| format | Article |
| id | mit-1721.1/83858 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:19:50Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/838582022-09-30T20:26:26Z Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy Mortensen, Luke J. Levy, Oren Phillips, Joseph P. Stratton, Tara Triana, Brian Ruiz, Juan P. Gu, Fangqi Lin, Charles Karp, Jeffrey Michael Harvard University--MIT Division of Health Sciences and Technology Karp, Jeffrey Michael The ability to deliver cells to appropriate target tissues is a prerequisite for successful cell-based therapy. To optimize cell therapy it is therefore necessary to develop a robust method of in vivo cell delivery quantification. Here we examine Mesenchymal Stem Cells (MSCs) labeled with a series of 4 membrane dyes from which we select the optimal dye combination for pair-wise comparisons of delivery to inflamed tissue in the mouse ear using confocal fluorescence imaging. The use of an optimized dye pair for simultaneous tracking of two cell populations in the same animal enables quantification of a test population that is referenced to an internal control population, thereby eliminating intra-subject variations and variations in injected cell numbers. Consistent results were obtained even when the administered cell number varied by more than an order of magnitude, demonstrating an ability to neutralize one of the largest sources of in vivo experimental error and to greatly reduce the number of cells required to evaluate cell delivery. With this method, we are able to show a small but significant increase in the delivery of cytokine pre-treated MSCs (TNF-α & IFN-γ) compared to control MSCs. Our results suggest future directions for screening cell strategies using our in vivo cell delivery assay, which may be useful to develop methods to maximize cell therapeutic potential. Sanofi Aventis (Firm) 2014-01-10T16:24:41Z 2014-01-10T16:24:41Z 2013-10 2013-05 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/83858 Mortensen, Luke J., Oren Levy, Joseph P. Phillips, Tara Stratton, Brian Triana, Juan P. Ruiz, Fangqi Gu, Jeffrey M. Karp, and Charles P. Lin. “Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy.” Edited by Irene Georgakoudi. PLoS ONE 8, no. 10 (October 29, 2013): e78145. en_US http://dx.doi.org/10.1371/journal.pone.0078145 PLoS ONE http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Mortensen, Luke J. Levy, Oren Phillips, Joseph P. Stratton, Tara Triana, Brian Ruiz, Juan P. Gu, Fangqi Lin, Charles Karp, Jeffrey Michael Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy |
| title | Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy |
| title_full | Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy |
| title_fullStr | Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy |
| title_full_unstemmed | Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy |
| title_short | Quantification of Mesenchymal Stem Cell (MSC) Delivery to a Target Site Using In Vivo Confocal Microscopy |
| title_sort | quantification of mesenchymal stem cell msc delivery to a target site using in vivo confocal microscopy |
| url | http://hdl.handle.net/1721.1/83858 |
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