Reconsidering Movement of Eukaryotic mRNAs between Polysomes and P Bodies
Cell survival in changing environments requires appropriate regulation of gene expression, including posttranscriptional regulatory mechanisms. From reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P bodies and can return to...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/83872 https://orcid.org/0000-0003-2807-9657 |
| _version_ | 1826193786577879040 |
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| author | Doudna, Jennifer A. Arribere, Joshua Alexander Gilbert, Wendy |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Doudna, Jennifer A. Arribere, Joshua Alexander Gilbert, Wendy |
| author_sort | Doudna, Jennifer A. |
| collection | MIT |
| description | Cell survival in changing environments requires appropriate regulation of gene expression, including posttranscriptional regulatory mechanisms. From reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P bodies and can return to active translation. We present evidence contradicting the notion that reversible storage of nontranslating mRNAs is a widespread and general phenomenon. First, genome-wide measurements of mRNA abundance, translation, and ribosome occupancy after glucose withdrawal show that most mRNAs are depleted from the cell coincident with their depletion from polysomes. Second, only a limited subpopulation of translationally repressed transcripts, comprising fewer than 400 genes, can be reactivated for translation upon glucose readdition in the absence of new transcription. This highly selective posttranscriptional regulation could be a mechanism for cells to minimize the energetic costs of reversing gene-regulatory decisions in rapidly changing environments by transiently preserving a pool of transcripts whose translation is rate-limiting for growth. |
| first_indexed | 2024-09-23T09:45:23Z |
| format | Article |
| id | mit-1721.1/83872 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:45:23Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/838722022-09-30T16:36:24Z Reconsidering Movement of Eukaryotic mRNAs between Polysomes and P Bodies Doudna, Jennifer A. Arribere, Joshua Alexander Gilbert, Wendy Massachusetts Institute of Technology. Department of Biology Arribere, Joshua Alexander Gilbert, Wendy Cell survival in changing environments requires appropriate regulation of gene expression, including posttranscriptional regulatory mechanisms. From reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P bodies and can return to active translation. We present evidence contradicting the notion that reversible storage of nontranslating mRNAs is a widespread and general phenomenon. First, genome-wide measurements of mRNA abundance, translation, and ribosome occupancy after glucose withdrawal show that most mRNAs are depleted from the cell coincident with their depletion from polysomes. Second, only a limited subpopulation of translationally repressed transcripts, comprising fewer than 400 genes, can be reactivated for translation upon glucose readdition in the absence of new transcription. This highly selective posttranscriptional regulation could be a mechanism for cells to minimize the energetic costs of reversing gene-regulatory decisions in rapidly changing environments by transiently preserving a pool of transcripts whose translation is rate-limiting for growth. National Institute of General Medical Sciences (U.S.) (Grant R00GM081399) 2014-01-10T20:45:38Z 2014-01-10T20:45:38Z 2011-12 2011-06 Article http://purl.org/eprint/type/JournalArticle 10972765 1097-4164 http://hdl.handle.net/1721.1/83872 Arribere, Joshua A., Jennifer A. Doudna, and Wendy V. Gilbert. “Reconsidering Movement of Eukaryotic mRNAs between Polysomes and P Bodies.” Molecular Cell 44, no. 5 (December 2011): 745-758. Copyright © 2011 Elsevier Inc. https://orcid.org/0000-0003-2807-9657 en_US http://dx.doi.org/10.1016/j.molcel.2011.09.019 Molecular Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier Open Archive |
| spellingShingle | Doudna, Jennifer A. Arribere, Joshua Alexander Gilbert, Wendy Reconsidering Movement of Eukaryotic mRNAs between Polysomes and P Bodies |
| title | Reconsidering Movement of Eukaryotic mRNAs between Polysomes and P Bodies |
| title_full | Reconsidering Movement of Eukaryotic mRNAs between Polysomes and P Bodies |
| title_fullStr | Reconsidering Movement of Eukaryotic mRNAs between Polysomes and P Bodies |
| title_full_unstemmed | Reconsidering Movement of Eukaryotic mRNAs between Polysomes and P Bodies |
| title_short | Reconsidering Movement of Eukaryotic mRNAs between Polysomes and P Bodies |
| title_sort | reconsidering movement of eukaryotic mrnas between polysomes and p bodies |
| url | http://hdl.handle.net/1721.1/83872 https://orcid.org/0000-0003-2807-9657 |
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