Using buoyant mass to measure the growth of single cells
We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/83911 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0002-8235-7227 |
| _version_ | 1826210910037868544 |
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| author | Godin, Michel Son, Sungmin Tzur, Amit Jorgensen, Paul Kirschner, Marc W. Delgado, Francisco Feijo Grover, William H. Bryan, Andrea Kristine Payer, Kristofor Robert Grossman, Alan D. Manalis, Scott R. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Godin, Michel Son, Sungmin Tzur, Amit Jorgensen, Paul Kirschner, Marc W. Delgado, Francisco Feijo Grover, William H. Bryan, Andrea Kristine Payer, Kristofor Robert Grossman, Alan D. Manalis, Scott R. |
| author_sort | Godin, Michel |
| collection | MIT |
| description | We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells. |
| first_indexed | 2024-09-23T14:57:43Z |
| format | Article |
| id | mit-1721.1/83911 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:57:43Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/839112022-10-01T23:35:14Z Using buoyant mass to measure the growth of single cells Godin, Michel Son, Sungmin Tzur, Amit Jorgensen, Paul Kirschner, Marc W. Delgado, Francisco Feijo Grover, William H. Bryan, Andrea Kristine Payer, Kristofor Robert Grossman, Alan D. Manalis, Scott R. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Microsystems Technology Laboratories Koch Institute for Integrative Cancer Research at MIT Godin, Michel Delgado, Francisco Feijo Son, Sungmin Grover, William H. Bryan, Andrea Kristine Payer, Kristofor Robert Grossman, Alan D. Manalis, Scott R. We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells. National Institutes of Health (U.S.) (MIT Center for Cell Decision Processes Grant P50GM68762) United States. Army Research Office (Institute for Collaborative Biotechnologies Grant DAAD1903D0004) 2014-01-13T19:06:33Z 2014-01-13T19:06:33Z 2010-04 2009-12 Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/83911 Godin, Michel, Francisco Feijó Delgado, Sungmin Son, William H Grover, Andrea K Bryan, Amit Tzur, Paul Jorgensen, et al. “Using buoyant mass to measure the growth of single cells.” Nature Methods 7, no. 5 (April 11, 2010): 387-390. https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0002-8235-7227 en_US http://dx.doi.org/10.1038/nmeth.1452 Nature Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Godin, Michel Son, Sungmin Tzur, Amit Jorgensen, Paul Kirschner, Marc W. Delgado, Francisco Feijo Grover, William H. Bryan, Andrea Kristine Payer, Kristofor Robert Grossman, Alan D. Manalis, Scott R. Using buoyant mass to measure the growth of single cells |
| title | Using buoyant mass to measure the growth of single cells |
| title_full | Using buoyant mass to measure the growth of single cells |
| title_fullStr | Using buoyant mass to measure the growth of single cells |
| title_full_unstemmed | Using buoyant mass to measure the growth of single cells |
| title_short | Using buoyant mass to measure the growth of single cells |
| title_sort | using buoyant mass to measure the growth of single cells |
| url | http://hdl.handle.net/1721.1/83911 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0002-8235-7227 |
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