FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions
The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Wiley Blackwell
2014
|
| Online Access: | http://hdl.handle.net/1721.1/84588 https://orcid.org/0000-0002-5749-7869 |
| _version_ | 1811092152185257984 |
|---|---|
| author | Imperiali, Barbara Socher, Elke Ricarda |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Imperiali, Barbara Socher, Elke Ricarda |
| author_sort | Imperiali, Barbara |
| collection | MIT |
| description | The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the donor is specifically bound to the protein of interest, eliminating high background and false positive signals. |
| first_indexed | 2024-09-23T15:13:48Z |
| format | Article |
| id | mit-1721.1/84588 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:13:48Z |
| publishDate | 2014 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/845882022-09-29T13:30:02Z FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions Imperiali, Barbara Socher, Elke Ricarda Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Socher, Elke Ricarda Imperiali, Barbara The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the donor is specifically bound to the protein of interest, eliminating high background and false positive signals. National Institutes of Health (U.S.) (Grant R01 EB010246) 2014-01-27T18:03:39Z 2014-01-27T18:03:39Z 2012-12 2012-11 Article http://purl.org/eprint/type/JournalArticle 14394227 1439-7633 http://hdl.handle.net/1721.1/84588 Socher, Elke, and Barbara Imperiali. “FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions.” ChemBioChem 14, no. 1 (January 2, 2013): 53-57. https://orcid.org/0000-0002-5749-7869 en_US http://dx.doi.org/10.1002/cbic.201200700 ChemBioChem Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Imperiali, Barbara Socher, Elke Ricarda FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions |
| title | FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions |
| title_full | FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions |
| title_fullStr | FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions |
| title_full_unstemmed | FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions |
| title_short | FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions |
| title_sort | fret capture a sensitive method for the detection of dynamic protein interactions |
| url | http://hdl.handle.net/1721.1/84588 https://orcid.org/0000-0002-5749-7869 |
| work_keys_str_mv | AT imperialibarbara fretcaptureasensitivemethodforthedetectionofdynamicproteininteractions AT socherelkericarda fretcaptureasensitivemethodforthedetectionofdynamicproteininteractions |