Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting
DNA methylation is an epigenetic mark associated with transposable element silencing and gene imprinting in flowering plants and mammals. In plants, imprinting occurs in the endosperm, which nourishes the embryo during seed development. We have profiled Arabidopsis DNA methylation genome-wide in the...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Association for the Advancement of Science (AAAS)
2014
|
| Online Access: | http://hdl.handle.net/1721.1/84596 https://orcid.org/0000-0003-2280-1522 |
| _version_ | 1811087094412476416 |
|---|---|
| author | Gehring, Mary Bubb, Kerry L. Henikoff, Steven |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Gehring, Mary Bubb, Kerry L. Henikoff, Steven |
| author_sort | Gehring, Mary |
| collection | MIT |
| description | DNA methylation is an epigenetic mark associated with transposable element silencing and gene imprinting in flowering plants and mammals. In plants, imprinting occurs in the endosperm, which nourishes the embryo during seed development. We have profiled Arabidopsis DNA methylation genome-wide in the embryo and endosperm and found that large-scale methylation changes accompany endosperm development and endosperm-specific gene expression. Transposable element fragments are extensively demethylated in the endosperm. We discovered new imprinted genes by the identification of candidates associated with regions of reduced endosperm methylation and preferential expression in endosperm relative to other parts of the plant. These data suggest that imprinting in plants evolved from targeted methylation of transposable element insertions near genic regulatory elements followed by positive selection when the resulting expression change was advantageous. |
| first_indexed | 2024-09-23T13:39:41Z |
| format | Article |
| id | mit-1721.1/84596 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:39:41Z |
| publishDate | 2014 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/845962022-09-28T15:21:28Z Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting Gehring, Mary Bubb, Kerry L. Henikoff, Steven Massachusetts Institute of Technology. Department of Biology Gehring, Mary DNA methylation is an epigenetic mark associated with transposable element silencing and gene imprinting in flowering plants and mammals. In plants, imprinting occurs in the endosperm, which nourishes the embryo during seed development. We have profiled Arabidopsis DNA methylation genome-wide in the embryo and endosperm and found that large-scale methylation changes accompany endosperm development and endosperm-specific gene expression. Transposable element fragments are extensively demethylated in the endosperm. We discovered new imprinted genes by the identification of candidates associated with regions of reduced endosperm methylation and preferential expression in endosperm relative to other parts of the plant. These data suggest that imprinting in plants evolved from targeted methylation of transposable element insertions near genic regulatory elements followed by positive selection when the resulting expression change was advantageous. 2014-01-27T19:09:50Z 2014-01-27T19:09:50Z 2009-06 2009-01 Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 http://hdl.handle.net/1721.1/84596 Gehring, M., K. L. Bubb, and S. Henikoff. “Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting.” Science 324, no. 5933 (June 11, 2009): 1447-1451. https://orcid.org/0000-0003-2280-1522 en_US http://dx.doi.org/10.1126/science.1171609 Science Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Association for the Advancement of Science (AAAS) PMC |
| spellingShingle | Gehring, Mary Bubb, Kerry L. Henikoff, Steven Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting |
| title | Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting |
| title_full | Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting |
| title_fullStr | Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting |
| title_full_unstemmed | Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting |
| title_short | Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting |
| title_sort | extensive demethylation of repetitive elements during seed development underlies gene imprinting |
| url | http://hdl.handle.net/1721.1/84596 https://orcid.org/0000-0003-2280-1522 |
| work_keys_str_mv | AT gehringmary extensivedemethylationofrepetitiveelementsduringseeddevelopmentunderliesgeneimprinting AT bubbkerryl extensivedemethylationofrepetitiveelementsduringseeddevelopmentunderliesgeneimprinting AT henikoffsteven extensivedemethylationofrepetitiveelementsduringseeddevelopmentunderliesgeneimprinting |