Methods for Purification of Proteins Associated with Cellular Poly(ADP-Ribose) and PARP-Specific Poly(ADP-Ribose)
available in PMC 2012 November 09
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Springer Science+Business Media
2014
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| Online Access: | http://hdl.handle.net/1721.1/84682 |
| _version_ | 1826189165495058432 |
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| author | Rood, Jennifer E. Leung, Anthony Chang, Paul |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Rood, Jennifer E. Leung, Anthony Chang, Paul |
| author_sort | Rood, Jennifer E. |
| collection | MIT |
| description | available in PMC 2012 November 09 |
| first_indexed | 2024-09-23T08:10:34Z |
| format | Article |
| id | mit-1721.1/84682 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:10:34Z |
| publishDate | 2014 |
| publisher | Springer Science+Business Media |
| record_format | dspace |
| spelling | mit-1721.1/846822022-09-23T11:23:47Z Methods for Purification of Proteins Associated with Cellular Poly(ADP-Ribose) and PARP-Specific Poly(ADP-Ribose) Rood, Jennifer E. Leung, Anthony Chang, Paul Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Rood, Jennifer E. Leung, Anthony Chang, Paul available in PMC 2012 November 09 Poly(ADP-ribose) (pADPr) is a posttranslational modification that regulates protein function through two major mechanisms: covalent modification of acceptor proteins and noncovalent binding of proteins to pADPr. pADPr is synthesized by a family of enzymes called poly(ADP-ribose) polymerases (PARPs) that are themselves major targets of pADPr modification. Here, we outline two methods for the purification of pADPr-binding proteins via pADPr purification under native conditions: purification of cellular pADPr and pADPr covalently linked to specific PARPs. Together, these methods provide complementary approaches to the identification of noncovalent pADPr–protein interactions in the cell. 2014-02-07T17:31:16Z 2014-02-07T17:31:16Z 2011 Article http://purl.org/eprint/type/JournalArticle 978-1-61779-269-4 978-1-61779-270-0 1064-3745 1940-6029 http://hdl.handle.net/1721.1/84682 Rood, Jennifer E., Anthony K. L. Leung, and Paul Chang. Methods for Purification of Proteins Associated with Cellular Poly(ADP-Ribose) and PARP-Specific Poly(ADP-Ribose). In Poly(ADP-Ribose) Polymerase: Methods and Protocols. Alexei V. Tulin (ed.). New York: Springer-Verlag, 2011. pp 153-164. (Methods in Molecular Biology; v.780). en_US http://dx.doi.org/10.1007/978-1-61779-270-0_10 Poly(ADP-ribose) Polymerase: Methods and Protocols Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Springer Science+Business Media PMC |
| spellingShingle | Rood, Jennifer E. Leung, Anthony Chang, Paul Methods for Purification of Proteins Associated with Cellular Poly(ADP-Ribose) and PARP-Specific Poly(ADP-Ribose) |
| title | Methods for Purification of Proteins Associated with Cellular Poly(ADP-Ribose) and PARP-Specific Poly(ADP-Ribose) |
| title_full | Methods for Purification of Proteins Associated with Cellular Poly(ADP-Ribose) and PARP-Specific Poly(ADP-Ribose) |
| title_fullStr | Methods for Purification of Proteins Associated with Cellular Poly(ADP-Ribose) and PARP-Specific Poly(ADP-Ribose) |
| title_full_unstemmed | Methods for Purification of Proteins Associated with Cellular Poly(ADP-Ribose) and PARP-Specific Poly(ADP-Ribose) |
| title_short | Methods for Purification of Proteins Associated with Cellular Poly(ADP-Ribose) and PARP-Specific Poly(ADP-Ribose) |
| title_sort | methods for purification of proteins associated with cellular poly adp ribose and parp specific poly adp ribose |
| url | http://hdl.handle.net/1721.1/84682 |
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