Oocyte differentiation is genetically dissociable from meiosis in mice
Oogenesis is the process by which ovarian germ cells undertake meiosis and differentiate to become eggs. In mice, Stra8 is required for the chromosomal events of meiosis to occur, but its role in differentiation remains unknown. Here we report Stra8-deficient ovarian germ cells that grow and differe...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2014
|
| Online Access: | http://hdl.handle.net/1721.1/85097 https://orcid.org/0000-0001-9920-3411 |
| _version_ | 1826203510574678016 |
|---|---|
| author | Dokshin, Gregoriy A. Baltus, Andrew E. Eppig, John J. Page, David C. Baltus, Andrew E. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Dokshin, Gregoriy A. Baltus, Andrew E. Eppig, John J. Page, David C. Baltus, Andrew E. |
| author_sort | Dokshin, Gregoriy A. |
| collection | MIT |
| description | Oogenesis is the process by which ovarian germ cells undertake meiosis and differentiate to become eggs. In mice, Stra8 is required for the chromosomal events of meiosis to occur, but its role in differentiation remains unknown. Here we report Stra8-deficient ovarian germ cells that grow and differentiate into oocyte-like cells that synthesize zonae pellucidae, organize surrounding somatic cells into follicles, are ovulated in response to hormonal stimulation, undergo asymmetric cell division to produce a polar body and cleave to form two-cell embryos upon fertilization. These events occur without premeiotic chromosomal replication, sister chromatid cohesion, synapsis or recombination. Thus, oocyte growth and differentiation are genetically dissociable from the chromosomal events of meiosis. These findings open to study the independent contributions of meiosis and oocyte differentiation to the making of a functional egg. |
| first_indexed | 2024-09-23T12:38:29Z |
| format | Article |
| id | mit-1721.1/85097 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:38:29Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/850972022-10-01T10:12:20Z Oocyte differentiation is genetically dissociable from meiosis in mice Dokshin, Gregoriy A. Baltus, Andrew E. Eppig, John J. Page, David C. Baltus, Andrew E. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Dokshin, Gregoriy A. Baltus, Andrew E. Page, David C. Oogenesis is the process by which ovarian germ cells undertake meiosis and differentiate to become eggs. In mice, Stra8 is required for the chromosomal events of meiosis to occur, but its role in differentiation remains unknown. Here we report Stra8-deficient ovarian germ cells that grow and differentiate into oocyte-like cells that synthesize zonae pellucidae, organize surrounding somatic cells into follicles, are ovulated in response to hormonal stimulation, undergo asymmetric cell division to produce a polar body and cleave to form two-cell embryos upon fertilization. These events occur without premeiotic chromosomal replication, sister chromatid cohesion, synapsis or recombination. Thus, oocyte growth and differentiation are genetically dissociable from the chromosomal events of meiosis. These findings open to study the independent contributions of meiosis and oocyte differentiation to the making of a functional egg. National Institutes of Health (U.S.) (HD23839) Howard Hughes Medical Institute 2014-02-26T17:59:54Z 2014-02-26T17:59:54Z 2013-06 2012-12 Article http://purl.org/eprint/type/JournalArticle 1061-4036 1546-1718 http://hdl.handle.net/1721.1/85097 Dokshin, Gregoriy A, Andrew E Baltus, John J Eppig, and David C Page. “Oocyte differentiation is genetically dissociable from meiosis in mice.” Nature Genetics 45, no. 8 (June 16, 2013): 877-883. https://orcid.org/0000-0001-9920-3411 en_US http://dx.doi.org/10.1038/ng.2672 Nature Genetics Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Nature Publishing Group PMC |
| spellingShingle | Dokshin, Gregoriy A. Baltus, Andrew E. Eppig, John J. Page, David C. Baltus, Andrew E. Oocyte differentiation is genetically dissociable from meiosis in mice |
| title | Oocyte differentiation is genetically dissociable from meiosis in mice |
| title_full | Oocyte differentiation is genetically dissociable from meiosis in mice |
| title_fullStr | Oocyte differentiation is genetically dissociable from meiosis in mice |
| title_full_unstemmed | Oocyte differentiation is genetically dissociable from meiosis in mice |
| title_short | Oocyte differentiation is genetically dissociable from meiosis in mice |
| title_sort | oocyte differentiation is genetically dissociable from meiosis in mice |
| url | http://hdl.handle.net/1721.1/85097 https://orcid.org/0000-0001-9920-3411 |
| work_keys_str_mv | AT dokshingregoriya oocytedifferentiationisgeneticallydissociablefrommeiosisinmice AT baltusandrewe oocytedifferentiationisgeneticallydissociablefrommeiosisinmice AT eppigjohnj oocytedifferentiationisgeneticallydissociablefrommeiosisinmice AT pagedavidc oocytedifferentiationisgeneticallydissociablefrommeiosisinmice AT baltusandrewe oocytedifferentiationisgeneticallydissociablefrommeiosisinmice |