Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation
Quality control of ribosomes is critical for cellular function since protein mistranslation leads to severe physiological consequences. We report evidence of a previously unrecognized ribosome quality control system in bacteria that operates at the level of 70S to remove defective ribosomes. YbeY, a...
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| Format: | Article |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/85187 https://orcid.org/0000-0001-7243-8261 https://orcid.org/0000-0002-4530-5647 |
| _version_ | 1826198716566994944 |
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| author | Davies, Bryan W. Koehrer, Caroline Walker, Graham C. Jacob, Asha I Rajbhandary, Uttam L |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Davies, Bryan W. Koehrer, Caroline Walker, Graham C. Jacob, Asha I Rajbhandary, Uttam L |
| author_sort | Davies, Bryan W. |
| collection | MIT |
| description | Quality control of ribosomes is critical for cellular function since protein mistranslation leads to severe physiological consequences. We report evidence of a previously unrecognized ribosome quality control system in bacteria that operates at the level of 70S to remove defective ribosomes. YbeY, a previously unidentified endoribonuclease, and the exonuclease RNase R act together by a process mediated specifically by the 30S ribosomal subunit, to degrade defective 70S ribosomes but not properly matured 70S ribosomes or individual subunits. Furthermore, there is essentially no fully matured 16S rRNA in a ΔybeY mutant at 45°C, making YbeY the only endoribonuclease to be implicated in the critically important processing of the 16S rRNA 3′ terminus. These key roles in ribosome quality control and maturation indicate why YbeY is a member of the minimal bacterial gene set and suggest that it could be a potential target for antibacterial drugs. |
| first_indexed | 2024-09-23T11:09:01Z |
| format | Article |
| id | mit-1721.1/85187 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:09:01Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/851872022-09-27T17:27:33Z Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation Davies, Bryan W. Koehrer, Caroline Walker, Graham C. Jacob, Asha I Rajbhandary, Uttam L Massachusetts Institute of Technology. Department of Biology Jacob, Asha I. Koehrer, Caroline Rajbhandary, Uttam L. Walker, Graham C. Quality control of ribosomes is critical for cellular function since protein mistranslation leads to severe physiological consequences. We report evidence of a previously unrecognized ribosome quality control system in bacteria that operates at the level of 70S to remove defective ribosomes. YbeY, a previously unidentified endoribonuclease, and the exonuclease RNase R act together by a process mediated specifically by the 30S ribosomal subunit, to degrade defective 70S ribosomes but not properly matured 70S ribosomes or individual subunits. Furthermore, there is essentially no fully matured 16S rRNA in a ΔybeY mutant at 45°C, making YbeY the only endoribonuclease to be implicated in the critically important processing of the 16S rRNA 3′ terminus. These key roles in ribosome quality control and maturation indicate why YbeY is a member of the minimal bacterial gene set and suggest that it could be a potential target for antibacterial drugs. National Institutes of Health (U.S.) (Grant GM31030) Deshpande Center for Technological Innovation GM17151 P30 ES002109 2014-02-28T16:39:27Z 2014-02-28T16:39:27Z 2012-12 2012-10 Article http://purl.org/eprint/type/JournalArticle 10972765 1097-4164 http://hdl.handle.net/1721.1/85187 Jacob, Asha Ivy, Caroline Kohrer, Bryan William Davies, Uttam Lal RajBhandary, and Graham Charles Walker. “Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation.” Molecular Cell 49, no. 3 (February 2013): 427–438. © 2013 Elsevier Inc. https://orcid.org/0000-0001-7243-8261 https://orcid.org/0000-0002-4530-5647 en_US http://dx.doi.org/10.1016/j.molcel.2012.11.025 Molecular Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier Elsevier Open Archive |
| spellingShingle | Davies, Bryan W. Koehrer, Caroline Walker, Graham C. Jacob, Asha I Rajbhandary, Uttam L Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation |
| title | Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation |
| title_full | Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation |
| title_fullStr | Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation |
| title_full_unstemmed | Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation |
| title_short | Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation |
| title_sort | conserved bacterial rnase ybey plays key roles in 70s ribosome quality control and 16s rrna maturation |
| url | http://hdl.handle.net/1721.1/85187 https://orcid.org/0000-0001-7243-8261 https://orcid.org/0000-0002-4530-5647 |
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