Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine
Proteasomes are the major energy-dependent proteolytic machines in the eukaryotic and archaeal domains of life. To execute protein degradation, the 20S core peptidase combines with the AAA+ ring of the 19S regulatory particle in eukarya or with the AAA+ proteasome-activating nucleotidase ring in som...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Association for the Advancement of Science
2014
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| Online Access: | http://hdl.handle.net/1721.1/85559 https://orcid.org/0000-0002-1719-5399 |
| _version_ | 1826209684969750528 |
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| author | Barthelme, Dominik Sauer, Robert T |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Barthelme, Dominik Sauer, Robert T |
| author_sort | Barthelme, Dominik |
| collection | MIT |
| description | Proteasomes are the major energy-dependent proteolytic machines in the eukaryotic and archaeal domains of life. To execute protein degradation, the 20S core peptidase combines with the AAA+ ring of the 19S regulatory particle in eukarya or with the AAA+ proteasome-activating nucleotidase ring in some archaea. Here, we find that Cdc48 and 20S from the archaeon Thermoplasma acidophilum interact to form a functional proteasome. Cdc48 is an abundant and essential double-ring AAA+ molecular machine ubiquitously present in archaea, where its function has been uncertain, and in eukarya where Cdc48 participates by largely unknown mechanisms in diverse cellular processes, including multiple proteolytic pathways. Thus, proteolysis in collaboration with the 20S peptidase may represent an ancestral function of the Cdc48 family. |
| first_indexed | 2024-09-23T14:27:10Z |
| format | Article |
| id | mit-1721.1/85559 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:27:10Z |
| publishDate | 2014 |
| publisher | American Association for the Advancement of Science |
| record_format | dspace |
| spelling | mit-1721.1/855592022-10-01T21:22:20Z Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine Barthelme, Dominik Sauer, Robert T Massachusetts Institute of Technology. Department of Biology Barthelme, Dominik Sauer, Robert T. Proteasomes are the major energy-dependent proteolytic machines in the eukaryotic and archaeal domains of life. To execute protein degradation, the 20S core peptidase combines with the AAA+ ring of the 19S regulatory particle in eukarya or with the AAA+ proteasome-activating nucleotidase ring in some archaea. Here, we find that Cdc48 and 20S from the archaeon Thermoplasma acidophilum interact to form a functional proteasome. Cdc48 is an abundant and essential double-ring AAA+ molecular machine ubiquitously present in archaea, where its function has been uncertain, and in eukarya where Cdc48 participates by largely unknown mechanisms in diverse cellular processes, including multiple proteolytic pathways. Thus, proteolysis in collaboration with the 20S peptidase may represent an ancestral function of the Cdc48 family. 2014-03-07T17:20:27Z 2014-03-07T17:20:27Z 2012-07 2012-05 Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 http://hdl.handle.net/1721.1/85559 Barthelme, D., and R. T. Sauer. “Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine.” Science 337, no. 6096 (August 16, 2012): 843-846. https://orcid.org/0000-0002-1719-5399 en_US http://dx.doi.org/10.1126/science.1224352 Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Association for the Advancement of Science PMC |
| spellingShingle | Barthelme, Dominik Sauer, Robert T Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine |
| title | Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine |
| title_full | Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine |
| title_fullStr | Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine |
| title_full_unstemmed | Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine |
| title_short | Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine |
| title_sort | identification of the cdc48•20s proteasome as an ancient aaa proteolytic machine |
| url | http://hdl.handle.net/1721.1/85559 https://orcid.org/0000-0002-1719-5399 |
| work_keys_str_mv | AT barthelmedominik identificationofthecdc4820sproteasomeasanancientaaaproteolyticmachine AT sauerrobertt identificationofthecdc4820sproteasomeasanancientaaaproteolyticmachine |